Antimicrobial, Antioxidant and Antiproliferative Properties of the Leaves of Senna siamea

Oyebade, K. F.; Daspan, A. J.; Denkok, Yohanna; Alemika, Taiwo E.; Ojerinde, Olalekan S.

doi:10.9734/jocamr/2021/v14i130235

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…This study suggests that the extract and compound isolates of S. siamea exhibit promising antioxidant activities which align with previous reports. In a study by Oyebade et al (2021) [71], the antioxidant properties of the leaf extracts of S. siamea were tested in vitro and the hexane, ethyl acetate, ethanol, and aqueous…”

Section: Antioxidant Activitymentioning

confidence: 99%

Exploring the medicinal potential of Senna siamea roots: an integrated study of antibacterial and antioxidant activities, phytochemical analysis, ADMET profiling, and molecular docking insights

Gebrehiwot,

Ensermu,

Dekebo

et al. 2024

Appl Biol Chem

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Nowadays, infectious diseases pose an alarming global threat to human health. The genus Senna is among the most well-known taxonomic categories commonly used in folk medicine to confront these challenges. Motivated by its traditional uses, a comprehensive study was conducted on the roots extract of Senna siamea, aiming to address the in vitro antibacterial and antioxidant efficacy of phytochemicals from the dichloromethane: methanol (1:1) roots extract of the plant, along with in silico computational studies. The separation of compounds was achieved using silica gel column chromatography. Whereas, the antibacterial and antioxidant activities were examined using paper disc diffusion and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays, respectively. Silica gel column chromatography of the dichloromethane: methanol (1:1) roots extract afforded lupeol (1), β-sitosterol (2a) and stigmasterol (2b), chrysophanol (3), betulinic acid (4), and glyceryl-1-hexacosanoate (5). Although these compounds have been previously reported from the plant, proof of their medicinal applications via in vitro and in silico studies is still lacking. Notably, our findings showed remarkable inhibition zones by the extract (18.00 ± 0.00 mm and 17.17 ± 0.24 mm) against E. coli and S. aureus, respectively, at 50 mg/mL compared to ciprofloxacin (23.33 ± 0.47 mm and 22.00 ± 0.00 mm, respectively), showcasing its potential antibacterial efficiency. Considerable inhibition zones were also recorded by chrysophanol (3) against E. coli (16.33 ± 0.24 mm) and S. pyogenes (16.00 ± 0.00 mm) at 2 mg/mL, compared to ciprofloxacin which showed 23.33 ± 0.47 mm and 21.67 ± 0.47 mm, respectively, signifying its potent antibacterial activities. In addition, the crude extract and chrysophanol (3) exhibited substantial IC50 values (1.24 and 1.71 µg/mL, respectively), suggesting their significant antioxidant potential compared to that of ascorbic acid (IC50: 0.53 µg/mL). Chrysophanol (3) fulfilled Lipinski’s rule with no violation and lupeol (1), β-sitosterol (2a), stigmasterol (2b), betulinic acid (4), and glyceryl-1-hexacosanoate (5) displayed one violation each which were in favor of the drug-likeness predictions. All the compounds exhibited no cytotoxicity and except betulinic acid (4), all the compounds also showed no carcinogenicity properties which were consistent with the prediction results of ciprofloxacin. The molecular docking computations revealed that all the compound isolates displayed strong and nearly strong binding affinities against all protein targets, ranging from − 6.6 kcal/mol to -9.2 kcal/mol (lupeol (1) against E. coli DNA gyrase B and topoisomerase II α, respectively). Thus, the present findings suggest the roots of Senna siamea for potential medicinal applications against multi-drug resistant pathogens hence validating its ethno-medicinal uses.

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Section: Antioxidant Activitymentioning

confidence: 99%

Exploring the medicinal potential of Senna siamea roots: an integrated study of antibacterial and antioxidant activities, phytochemical analysis, ADMET profiling, and molecular docking insights

Gebrehiwot,

Ensermu,

Dekebo

et al. 2024

Appl Biol Chem

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“…Hasil penelitian sebelumnya menyebutkan bahwa aktivitas antioksidan pada ekstrak etanol daun johar (Cassia siamea Lam.) memiliki nilai IC50 yaitu 25,73 µg/ml (Oyebade et al, 2021). Aktivitas antioksidan dapat ditemukan pada senyawa flavonoid.…”

Section: Pendahuluanunclassified

OPTIMASI Na-CMC SEBAGAI GELLING AGENT DAN PROPILEN GLIKOL SEBAGAI HUMEKTAN PADA SEDIAAN GEL EKSTRAK DAUN JOHAR (Cassia siamea Lam.)

Putri,

Setiyadi

2023

ujp

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Ekstrak daun johar (Cassia siamea Lam.) sebelumnya telah diteliti mempunyai kemampuan antioksidan yang baik. Ekstrak ini dapat berpotensi untuk dijadikan sebagai komponen bahan aktif dalam pengembangan sediaan topikal seperti gel. Untuk mendapatkan sediaan gel yang sesuai harapan dapat dilakukan dengan optimasi. Tujuan dari penelitian yaitu untuk mengatahui nilai konsentrasi optimal bahan Na-CMC sebagai gelling agent dan propilen glikol sebagai humektan berdasarkan sifat fisik seperti viskositas, daya sebar, daya lekat, pH dan sifat sineresis dari sediaan gel ekstrak daun johar (Cassia siamea Lam.) dan aktivitas antioksidannya. Metode yang digunakan pada optimasi ini yaitu dengan D-optimal design pada level tinggi dan level rendah. Formula optimum dibuat dari pencampuran bahan Na-CMC, propilen glikol, ekstrak daun johar, metil paraben, BHT dan aquadest. Respon yang diukur yaitu viskositas, daya sebar daya lekat, pH serta aktivitas antioksidan pada formula optimal. Percobaan menunjukkan hasil formula optimum gel dengan Na-CMC 3% dan propilen glikol 15%. Hasil respon dari percobaan uji viskositas, uji daya sebar, uji daya lekat dan uji pH dengan rata-rata standar deviasi berturut-turut yaitu 31,92±836,109 cps; 4,25±0,177 cm; 5±10,66 detik; dan 4,64±0,037. Nilai IC50 pada hasil gel ekstrak daun johar yang optimal yaitu 58,98 g/ml, pada formula gel ekstrak daun johar yang optimal terdapat adanya aktivitas antioksidan.

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“…Hasil penelitian ini sejalan dengan hasil penelitian Fitriah et al (2017), ekstrak etanol daun johar pada konsentrasi 100% menunjukkan diameter zona hambat terhadap Staphylococcus aureus sebesar 14,9 mm dan penelitian Oyebade et al (2021) yang menunjukkan kemampuan antibakteri ekstrak etanol daun johar terhadap Staphylococcus aureus pada konsentrasi 50% menghasilkan diameter zona hambat sebesar 10,0 mm.…”

Section: Pemastian Bakteriunclassified

“…Juga sesuai dengan penelitian Oyebade et al (2021) yang menyatakan ekstrak etil asetat daun johar hasil maserasi bertingkat dengan cara yang sama seperti di atas, mengandung flavonoid.…”

Section: Nounclassified

Active Antibacterial Fraction from Ethanolic Extract of Johar Leaves (Cassia siamea Lam.) against Staphylococcus epidermidis and Its Bioautography

Munawaroh,

Khoirunnisa

2023

Pharmacon

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Johar leaves (Cassia siamea Lam.) have been traditionally used to treat pruritus due to their antibacterial properties. This study aims to identify the active fraction of an ethanolic extract of Johar leaves against Staphylococcus epidermidis, as well as the active chemical class. The extraction process involved maceration with 96% ethanol. The liquid-liquid extraction method was used to separate the n-hexane, ethyl acetate, and water fractions. The resulting extract and fraction were assessed for antibacterial activity using the well diffusion method. Sample loadings of 4 mg, 2 mg and 1 mg per well were used, with DMSO as a negative control and 6 mg chloramphenicol as a positive control. The test results indicate that the ethyl acetate fraction is more effective than the extract. Specifically, the fraction produced an inhibition zone of 13.5 ± 0.5 mm at a loading of 4 mg/well, while the extract only produced an inhibition zone of 9.3 ± 1.2 mm at the same loading. Additionally, there was a significant difference in the diameter of the inhibitory zone between treatment groups at p 0.05. The TLC results indicate that the ethyl acetate fraction of Johar leaves contains alkaloid, phenolic, flavonoid, and steroid compounds when using silica gel 60 F254 stationary phase and chloroform:ethyl acetate:ethanol (7:2:1 v/v/v) mobile phase. Bioautography results showed that the phenolic and alkaloid compounds at Rf 0.22 and phenolic compound at Rf 0.42 are responsible for the antibacterial activity against Staphylococcus epidermidis.

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Antimicrobial, Antioxidant and Antiproliferative Properties of the Leaves of Senna siamea

Cited by 3 publications

References 7 publications

Exploring the medicinal potential of Senna siamea roots: an integrated study of antibacterial and antioxidant activities, phytochemical analysis, ADMET profiling, and molecular docking insights

Exploring the medicinal potential of Senna siamea roots: an integrated study of antibacterial and antioxidant activities, phytochemical analysis, ADMET profiling, and molecular docking insights

OPTIMASI Na-CMC SEBAGAI GELLING AGENT DAN PROPILEN GLIKOL SEBAGAI HUMEKTAN PADA SEDIAAN GEL EKSTRAK DAUN JOHAR (Cassia siamea Lam.)

Active Antibacterial Fraction from Ethanolic Extract of Johar Leaves (Cassia siamea Lam.) against Staphylococcus epidermidis and Its Bioautography

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