Antimicrobial, Antioxidant and Hemolytic Activity of Water-soluble Extract of Mottled Anemone Urticina crassicornis

Lee, Ye-Jin; Kim, Chan‐Hee; Oh, Hye Young; Go, Hye‐Jin; Park, Nam Gyu

doi:10.5657/fas.2015.0341

Cited by 2 publications

(3 citation statements)

References 37 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extracts of Urticina crassicornis showed antioxidant and hemolytic activity (Lee et al 2015). Moreover, a novel biopeptide τ-AnmTx Ueq 12-1 was discovered from Urticina eques.…”

Section: Order Actiniariamentioning

confidence: 97%

Novel marine bioactives: application in functional foods, nutraceuticals, and pharmaceuticals

Shahidi

Santhiravel

2022

Journal of Food Bioactives

View full text Add to dashboard Cite

Functional food, nutraceutical, and pharmaceutical applications of natural products have gained growing attention as there is increasingly awareness of the association between bioactive compounds and improved health. Recently, food and biomedical scientists have focused more on marine resources to isolate bioactives since the marine ecosystem comprises unexploited resources with a wide range of organisms. Marine species produce a wide range of natural products, such as polysaccharides, peptides, polyunsaturated lipids, phenolic compounds, and pigments, with unique structures and diverse biological activities due to their extreme living environments. These active molecules reduce the risk of chronic disease and improve health by exhibiting antioxidant, anticancer, anti-inflammatory, antimicrobial, antidiabetic, anti-obesity, antihypertensive, cardioprotective, and neuroprotective activities. This review summarizes the recent discoveries of bioactive compounds from marine invertebrates (sponges, cnidarians, echinoderms, molluscs, ascidians, and crustaceans), fishes, seaweeds, and marine microorganisms and their potential for functional foods, nutraceuticals, and pharmaceuticals applications.

show abstract

“…The extracts of Urticina crassicornis showed antioxidant and hemolytic activity (Lee et al 2015). Moreover, a novel biopeptide τ-AnmTx Ueq 12-1 was discovered from Urticina eques.…”

Section: Order Actiniariamentioning

confidence: 97%

Novel marine bioactives: application in functional foods, nutraceuticals, and pharmaceuticals

Shahidi

Santhiravel

2022

Journal of Food Bioactives

View full text Add to dashboard Cite

show abstract

“…We recently evaluated the antimicrobial activity of various tissue extracts, including mucus, from northern Pacific sea anemone (Urticina crassicornis) against gram-positive and gram-negative bacteria. The antimicrobial activity of all tissue extracts was evident without any hemolytic activity against human red blood cells, but was completely abolished by treatment with proteolytic enzymes, suggesting that the extracts contained proteinaceous antimicrobial materials and represent a proper source for biochemical isolation of AMPs [26].…”

Section: Introductionmentioning

confidence: 98%

“…The antimicrobial activity of all tissue extracts was evident without any hemolytic activity against human red blood cells, but was completely abolished by treatment with proteolytic enzymes, suggesting that the extracts contained proteinaceous antimicrobial materials and represent a proper source for biochemical isolation of AMPs [26]. We recently evaluated the antimicrobial activity of various tissue extracts, including mucus, from northern Pacific sea anemone (Urticina crassicornis) against gram-positive and gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

Defensin‐neurotoxin dyad in a basally branching metazoan sea anemone

Kim

Lee

et al. 2017

The FEBS Journal

Self Cite

View full text Add to dashboard Cite

Recent studies suggest that vertebrate and invertebrate defensins have evolved from two independent ancestors, and that both defensins could share origins with animal toxins. Here, we purified novel sea anemone neurotoxin (BDS)-like antimicrobial peptides (AMPs)-Crassicorin-I and its putative homolog (Crassicorin-II)-from the pharynx extract of an anthozoan sea anemone (Urticina crassicornis). Based on structural analyses and cDNA cloning, mature Crassicorin-I represents a cationic AMP likely generated from a precursor and comprising 40 amino acid residues, including six cysteines forming three intramolecular disulfide bonds. Recombinant Crassicorin-I produced in a heterologous bacterial-expression system displayed antimicrobial activity against both a gram-positive bacterium (Bacillus subtilis) and gram-negative bacteria (Escherichia coli and Salmonella enterica). The Crassicorin-I transcript was upregulated by immune challenge, suggesting its involvement in defense mechanisms against infectious pathogens in sea anemone. Sequence alignment and three-dimensional molecular modeling revealed that Crassicorin-I exhibits high degrees of structural similarity to sea anemone neurotoxins that share β-defensin fold which is found in vertebrate defensins and invertebrate big-defensins. Consistent with its structural similarity to neurotoxins, Crassicorin-I exhibited paralytic activity toward a crustacean. These findings motivated our investigation and subsequent discovery of antimicrobial activity from other known sea anemone neurotoxins, such as APETx1 and ShK. Collectively, our work signified that Crassicorin-I is the first AMP identified from a sea anemone and provided evidence of a functional linkage between AMPs and neurotoxins in a basally branching metazoan.

show abstract

Antimicrobial, Antioxidant and Hemolytic Activity of Water-soluble Extract of Mottled Anemone Urticina crassicornis

Cited by 2 publications

References 37 publications

Novel marine bioactives: application in functional foods, nutraceuticals, and pharmaceuticals

Novel marine bioactives: application in functional foods, nutraceuticals, and pharmaceuticals

Defensin‐neurotoxin dyad in a basally branching metazoan sea anemone

Contact Info

Product

Resources

About