Antimicrobial, antioxidant, anti-inflammatory and cytotoxic study of extracts of Guibourtia tessmanii (harms) J. Léonard from Gabon

Obiang, Cédric Sima; Misso, Rick Léonid Ngoua Meye; Atome, Guy Roger Ndong; Obame, Rany Berty Mbeng; Ondo, Joseph Privat; Engonga, Louis Clément Obame; Emvo, Edouard Nsi

doi:10.1186/s40816-021-00278-0

Cited by 8 publications

(8 citation statements)

References 24 publications

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“…The study also shows that the toxicity is progressive over time. This result corroborates with that of Obiang et al [ 11 ], where the authors stipulate that the mortality correlated to the doses used is all the more increased as the exposure of frog tadpoles is prolonged over time. The cells observed on the smears correspond quite closely to the descriptions made in leopard frogs by Jordan [ 20 ].…”

Section: Discussionsupporting

confidence: 91%

Toxicity, Antibacterial, and Phytochemical Analyses of Antrocaryon klaineanum Pierre Extracts

Obiang

Mba

Ondo

et al. 2023

Advances in Pharmacological and Pharmaceutical Sciences

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Medicinal plants are traditionally used in Gabon to treat several types of illnesses. The study’s purpose was to determine the toxic, antibacterial, and anti-inflammatory effects of Antrocaryon klaineanum Pierre extracts and to characterize their phytochemical compounds. Toxicity was evaluated on frog tadpoles (Phrynobatrachus africanus Hallowell). The microorganism susceptibility test was performed by the diffusion method, while minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated using the microdilution technique. Anti-inflammatory activity was tested through protein denaturation and membrane stabilization methods. Chromatography and molecular network techniques were used to characterize chemical compounds. The lethality test showed that the lethal concentration (LC50) increased from 110.03 ± 1.25 to 15.86 ± 2.21 μg/mL after 24 and 96 hours of exposure. In tadpoles exposed to 7.81 μg/mL extract, the first mortalities (12.5%) were observed on the fifth day of exposure. A relative decrease in mature erythrocytes exposed to plant extracts was observed. The antibacterial activity shows that the Ak F2, Ak F3, and Ak F4 fractions (from the water-ethanol crude extract) gave the greatest antibacterial activities compared to the other extracts. The water, water-acetone, and water-ethanol extracts showed good inhibition of denaturation. The haemolysis test shows that the extracts exhibited good anti-inflammatory activities. Phytochemical characterisation revealed four major compounds, including monogallate epicatechin and hydroxy-ergostadian. The molecular network revealed five main clusters. Our study shows that A. klaineanum Pierre could be a promising natural product for the isolation of molecules with potential biological activities.

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Section: Discussionsupporting

confidence: 91%

Toxicity, Antibacterial, and Phytochemical Analyses of Antrocaryon klaineanum Pierre Extracts

Obiang

Mba

Ondo

et al. 2023

Advances in Pharmacological and Pharmaceutical Sciences

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“…The potent antioxidant activities reported in this study may be attributable to the substantial presence of total phenolic and flavonoids in the LLFPME. The antioxidants act through the transfer of hydrogen atoms and by transfer of electrons or chelating of metals [29,30]. The LLFPME showed strong DPPH scavenging activity, ferric reducing antioxidant power and total antioxidant capacity; which compared favourably with the standard drugs used and are consistent with earlier studies [28,29,31,32].…”

Section: Discussionsupporting

confidence: 89%

Studies on Phytochemicals and Bioactivities of Methanolic Extract of Leucaena leucocephala (Lam.) de Wit Fruit Pods

Odekanyin,

Abioye,

Fajobi

et al. 2024

AJRB

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Objectives: The study was designed to screen for the phytochemical constituents, and investigate the antioxidant, antibacterial, and anti-inflammatory activities of Leucaena leucocephala fruit pods methanolic extract (LLFPME). Materials and Methods: Matured and dried fruit pods were pulverized and extracted with methanol using maceration method. The filtrate was concentrated in a rotary evaporator under reduced pressure to obtain powdery form termed LLFPME. The LLFPME was tested for the presence of phytochemicals using TLC and GC-MS technique. The phenolic and flavonoid contents of the extract were quantified; the antioxidant potential of the extract was examined using DPPH and H2O2 radical scavenging, metal chelating, FRAP and TAC assays. The anti-inflammatory potential was evaluated using the membrane stabilizing and lipoxygenase inhibitory activities while the antibacterial activity was investigated by determining the zone of inhibition, minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of extract against the test bacterial isolates. Results: TLC revealed the presence of flavonoids, saponins, alkaloids, tannins, and others while the GC-MS analysis also showed the presence of 27 compounds. It was shown that the LLFPME displayed measurable antioxidant activity by exhibiting DPPH and H2O2 free radical scavenging potential and dose dependent metal chelating ability. FRAP and TAC were estimated to be 14.88 ± 0.04 and 25.53 ± 0.21 mgAAE/g of extract, respectively. The extract protected the RBC membrane integrity of RBC subjected to heat and hypotonic stress; and inhibited the activity of lipoxygenase. The extract inhibited the growth of some bacterial strains but had no visible effect on Pseudomonas aeuroginosa growth. The zone of inhibition, MIC and MBC exhibited by the extract against susceptible test bacteria ranges between 13 - 17 mm, 2.5 - 20 and 20 mg/ml, respectively.. Conclusion: It was concluded that LLFPME exhibited antioxidant potential through various mechanisms that possibly aided its ability to stabilize stressed cell membrane and similarly inhibited the growth of some bacterial strain.

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“…In order to determine the bactericidal or bacteriostatic modes of action of the tested samples, the MBC/MIC ratios were calculated. The results obtained were interpreted as follows: when the MBC/MIC ratio was between 1 and 2, the extract or fraction was bactericidal, and when this ratio was between 4 and 16, the extract was considered bacteriostatic [ 27 ]. On this basis, the results in Table 4 show that fraction Bt EtOH F2 is bactericidal against Escherichia coli ATCC 8739, Escherichia coli ATCC 25992 and Salmonella enterica (MBC/MIC ratio = 2) and bacteriostatic against Klebsiella pneumoniae MDR and Escherichia coli ESBL (MBC/MIC ratio = 4).…”

Section: Resultsmentioning

confidence: 99%

“…The minimum inhibitory concentrations (MICs) of the crude extracts and fractions were determined with the microdilution method on 96-well microplates [ 27 ]. A series of seven dilutions of each extract (double dilutions ranging from 0.0049 to 5 mg/mL) were made in Muller–Hinton Nutrient Broth (Liofilchem, Roseto, Italy).…”

Section: Methodsmentioning

confidence: 99%

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Cytotoxicity and Identification of Antibacterial Compounds from Baillonella toxisperma Bark Using a LC-MS/MS and Molecular Networking Approach

et al. 2023

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Baillonella toxisperma is a medicinal plant used in northern Gabon to treat microbial diseases. It is a plant well-known by local populations, but very few studies have focused on the molecules responsible for the antibacterial activities of B. toxisperma. This study proposes a dereplication strategy based on molecular networking generated from HPLC-ESI-Q/TOF data, allowing investigation of the molecules responsible for the antibacterial activity of B. toxisperma. From this strategy, eighteen compounds were putatively identified. All of these compounds belonged mainly to five families of natural compounds, including phenylpropanolamines, stilbenes, flavonoids, lignans and phenolic glycosides. The chemical study carried out from the bark of B. toxisperma allowed us to identify, for the first time, compounds such as resveratrol and derivatives, epicatechin, epigallocatechin and epigallocatechin gallate. In addition, antibacterial activity (diffusion method and microdilution) and cytotoxicity (Cell Counting Kit-8 (CCK-8 Assay)) in vitro were evaluated. The crude ethanolic extract, as well as the fractions of B. toxisperma, showed significant antibacterial activity. However, the ethanolic fractions F2 and F4 presented high antibacterial activity compared to the crude extract. Cytotoxicity studies on colon-cancer cells (Caco-2) and human keratinocyte cells (HaCaT) showed moderate cytotoxicity in both cell types. This study clearly shows the therapeutic potential of the ethanolic extract of the bark of B. toxisperma and provides information on the phytochemical composition and bioactive compounds of the plant.

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Antimicrobial, antioxidant, anti-inflammatory and cytotoxic study of extracts of Guibourtia tessmanii (harms) J. Léonard from Gabon

Cited by 8 publications

References 24 publications

Toxicity, Antibacterial, and Phytochemical Analyses of Antrocaryon klaineanum Pierre Extracts

Toxicity, Antibacterial, and Phytochemical Analyses of Antrocaryon klaineanum Pierre Extracts

Studies on Phytochemicals and Bioactivities of Methanolic Extract of Leucaena leucocephala (Lam.) de Wit Fruit Pods

Cytotoxicity and Identification of Antibacterial Compounds from Baillonella toxisperma Bark Using a LC-MS/MS and Molecular Networking Approach

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