Antimicrobial Chitosan Nanodroplets: new Insights for Ultrasound-Mediated Adjuvant Treatment of Skin Infection

Banche, Giuliana; Prato, Maurizio; Magnetto, C.; Allizond, Valeria; Giribaldi, Giuliana; Argenziano, Monica; Khadjavi, Amina; Gulino, Giulia Rossana; Finesso, Nicole; Mandras, Narcisa; Tullio, Vivian; Cavalli, Roberta; Guiot, Caterina; Cuffini, Annamaria

doi:10.2217/fmb.15.27

Cited by 32 publications

(20 citation statements)

References 41 publications

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“…Some investigations involve combining nanoparticles and antibacterial agents for the synergistic inhibition of MRSA infection. For instance, Banche et al (2015) developed chitosan nanodroplets loaded with oxygen for efficiently eradicating MRSA and Candida albicans without resultant cytotoxicity on keratinocytes. Argenziano et al (2017) demonstrated that ultrasound-mediated vancomycin-loaded nanobubbles were more effective than free vancomycin for killing MRSA.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Anti-MRSA Activity of Cationic Nanostructured Lipid Carriers in Combination With Oxacillin for Cutaneous Application

Alalaiwe

Wang

et al. 2018

Front. Microbiol.

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Nanoparticles have become a focus of interest due to their ability as antibacterial agents. The aim of this study was to evaluate the anti-methicillin-resistant Staphylococcus aureus (MRSA) activity of cationic nanostructured lipid carriers (NLC) combined with oxacillin against ATCC 33591 and clinical isolate. The cationic resource on the NLC surface was soyaethyl morpholinium ethosulfate (SME). NLC loaded with oxacillin was produced to assess the antibacterial activity and the effectiveness of topical application for treating cutaneous infection. The hydrodynamic diameter and zeta potential of oxacillin-loaded NLC were 177 nm and 19 mV, respectively. When combined with NLC, oxacillin exhibited synergistic MRSA eradication. After NLC encapsulation, the minimum bactericidal concentration (MBC) of oxacillin decreased from 250 to 62.5 μg/ml. The combined NLC and oxacillin reduced the MRSA biofilm thickness from 31.2 to 13.0 μm, which was lower than the effect of NLC (18.2 μm) and antibiotic (25.2 μm) alone. The oxacillin-loaded NLC showed significant reduction in the burden of intracellular MRSA in differentiated THP-1 cells. This reduction was greater than that achieved with individual treatment. The mechanistic study demonstrated the ability of cationic NLC to disrupt the bacterial membrane, leading to protein leakage. The cell surface disintegration also increased oxacillin delivery into the cytoplasm, activating the bactericidal process. Topical NLC treatment of MRSA abscess in the skin decreased the bacterial load by log 4 and improved the skin’s architecture and barrier function. Our results demonstrated that a combination of nanocarriers and an antibiotic could synergistically inhibit MRSA growth.

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Section: Introductionmentioning

confidence: 99%

Synergistic Anti-MRSA Activity of Cationic Nanostructured Lipid Carriers in Combination With Oxacillin for Cutaneous Application

Alalaiwe

Wang

et al. 2018

Front. Microbiol.

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“…Both nanodevices, coated with biocompatible and biodegradable polysaccharides such as dextran sulfate or chitosan, proved to be effective in oxygen delivery either in vitro or in vivo (7)(8)(9)(10). Chitosan-shelled/decafluoropentane-cored oxygen-loaded nanodroplets (OLN), having average diameters of about 700 nm, positive charge and long-term stability (7) have been designed as potential therapeutic tools to promote healing processes in infected chronic wounds, since chitosan-based OLN displayed antimicrobial properties against methylcillin-resistant Staphylococcus aureus and Candida albicans (11). Interestingly, this types of OLN showed the capability to overcome hypoxia-dependent dysregulation of matrix-associated proteolytic enzymes such as matrix metalloproteinases (12).…”

Section: Introductionmentioning

confidence: 99%

‘In Vitro’, ‘In Vivo’ and ‘In Silico’ Investigation of the Anticancer Effectiveness of Oxygen-Loaded Chitosan-Shelled Nanodroplets as Potential Drug Vector

et al. 2018

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“…Firstly, the as-prepared samples are biocompatible regardless of DCF concentration, which is the highest after 360 minutes, and regardless of possible CHI degradation products that could occur during the same period of time. CHI has already (as mentioned above) been proven to be biocompatible 87 88 108 . And secondly, these results are very promising with regard to our desire to prepare coatings on AISI 316LVM stainless steel that would promote osteointegration.…”

Section: Resultsmentioning

confidence: 95%

“…Systemic therapy with regard to pain reduction is always employed after orthopedic surgery, while immunomodulatory and/or anti-inflammatory drugs to lower the possibility of implant rejection by the body are administered if necessary. Last but not least, it was previously proven that CHI provides antimicrobial activity 87 88 . The latter could additionally lower the possibility of complications related to the hip replacement procedure, since our approach would prevent initial infection during the procedure, whereas consecutive infections, following surgery, are always handled systemically due to the possible occurrence of antimicrobial resistance with locally applied antibiotics 89 .…”

Section: Resultsmentioning

confidence: 99%

Novel chitosan/diclofenac coatings on medical grade stainless steel for hip replacement applications

Finšgar

Uzunalić

Stergar

et al. 2016

Sci Rep

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Corrosion resistance, biocompatibility, improved osteointegration, as well the prevention of inflammation and pain are the most desired characteristics of hip replacement implants. In this study we introduce a novel multi-layered coating on AISI 316LVM stainless steel that shows promise with regard to all mentioned characteristics. The coating is prepared from alternating layers of the biocompatible polysaccharide chitosan and the non-steroid anti-inflammatory drug (NSAID), diclofenac. Electrochemical methods were employed to characterize the corrosion behavior of coated and uncoated samples in physiological solution. It is shown that these coatings improve corrosion resistance. It was also found that these coatings release the incorporated drug in controlled, multi-mechanism manner. Adding additional layers on top of the as-prepared samples, has potential for further tailoring of the release profile and increasing the drug dose. Biocompatibility was proven on human-derived osteoblasts in several experiments. Only viable cells were found on the sample surface after incubation of the samples with the same cell line. This novel coating could prove important for prolongation of the application potential of steel-based hip replacements, which are these days often replaced by more expensive ceramic or other metal alloys.

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Antimicrobial Chitosan Nanodroplets: new Insights for Ultrasound-Mediated Adjuvant Treatment of Skin Infection

Abstract: As such, US-activated chitosan-shelled OLNs appear as promising, nonconventional and innovative tools for adjuvant treatment of infected chronic wounds.

Cited by 32 publications

References 41 publications

Synergistic Anti-MRSA Activity of Cationic Nanostructured Lipid Carriers in Combination With Oxacillin for Cutaneous Application

Synergistic Anti-MRSA Activity of Cationic Nanostructured Lipid Carriers in Combination With Oxacillin for Cutaneous Application

‘In Vitro’, ‘In Vivo’ and ‘In Silico’ Investigation of the Anticancer Effectiveness of Oxygen-Loaded Chitosan-Shelled Nanodroplets as Potential Drug Vector

Novel chitosan/diclofenac coatings on medical grade stainless steel for hip replacement applications

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