Antimicrobial Coatings from Hybrid Nanoparticles of Biocompatible and Antimicrobial Polymers

Galvão, Carolina Nascimento; Sanches, Luccas Missfeldt; Mathiazzi, Beatriz Ideriha; Ribeiro, Rodrigo T.; Petri, Denise Freitas Siqueira; Carmona-Ribeiro, Ana Maria

doi:10.3390/ijms19102965

Cited by 39 publications

(61 citation statements)

References 40 publications

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“…Recently, we described hybrid NPs made of poly (methyl methacrylate) (PMMA) in the presence of poly (diallyl dimethyl ammonium) chloride (PDDA) using dioctadecyl dimethyl ammonium bromide (DODAB) as an emulsifier and found that DODAB remains attached to the NPs core [56][57][58][59]. This system might bring interesting applications for antigen presentation since DODAB might eventually add the missing Th-1 response to the antigen-presenting NPs.…”

Section: Immunoadjuvant Properties Of Pdda/ova Npsmentioning

confidence: 99%

Simple Nanoparticles from the Assembly of Cationic Polymer and Antigen as Immunoadjuvants

et al. 2020

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Since antigens are negatively charged, they combine well with positively charged adjuvants. Here, ovalbumin (OVA) (0.1 mg·mL −1 ) and poly (diallyldimethylammonium chloride) (PDDA) (0.01 mg·mL −1 ) yielded PDDA/OVA assemblies characterized by dynamic light scattering (DLS) and scanning electron microscopy (SEM) as spherical nanoparticles (NPs) of 170 ± 4 nm hydrodynamic diameter, 30 ± 2 mV of zeta-potential and 0.11 ± 0.01 of polydispersity. Mice immunization with the NPs elicited high OVA-specific IgG1 and low OVA-specific IgG2a production, indicating a Th-2 response. Delayed-type hypersensitivity reaction (DTH) was low and comparable to the one elicited by Al(OH) 3 /OVA, suggesting again a Th-2 response. PDDA advantages as an adjuvant were simplicity (a single-component adjuvant), low concentration needed (0.01 mg·mL −1 PDDA) combined with antigen yielding neglectable cytotoxicity, and high stability of PDDA/OVA dispersions. The NPs elicited much higher OVA-specific antibodies production than Al(OH) 3 /OVA. In vivo, the nano-metric size possibly assured antigen presentation by antigen-presenting cells (APC) at the lymph nodes, in contrast to the location of Al(OH) 3 /OVA microparticles at the site of injection for longer periods with stimulation of local dendritic cells. In the future, it will be interesting to evaluate combinations of the antigen with NPs carrying both PDDA and elicitors of the Th-1 response.Vaccines 2020, 8, 105 2 of 16 or cationic polymers on superparamagnetic iron oxide NPs [18] have also been proposed as effective micro or nanomaterials able to effectively interact with antigens and antigen-presenting cells (APC).Major mechanisms for taking up the cationic assemblies depend on size [19,20]. Nanoparticles with 20-200 nm mean diameter are like viruses and usually taken up by endocytosis via clathrin-coated vesicles, caveolae, or their independent receptors, and preferentially ingested by dendritic cells (DC) [21]. Microparticles with 500-5000 nm diameter are like bacteria, captured by phagocytosis and primarily ingested by macrophages. All particles used in vaccine formulations are consequently internalized efficiently by APC by one or a combination of the quoted mechanisms [22,23]. Particles with diameters smaller than 500 nm, in particular the nano-sized ones with 40-100 nm diameter, are more efficient to promote CD8 and CD4 type 1 T-helper cells responses than those with diameters above 500 nm; large particles, more similar to Al (OH) 3 , usually induce good T-helper cells type 2 improvement in antibody responses [22]. In summary, cationic micro and nanoparticles are effectively taken up both by macrophages and dendritic cells since electrostatic attraction promotes particle binding and subsequent internalization [11,14,20,24,25]. Cationic particles and liposomes containing dioctadecyldimethylammonium bromide (DODAB) cationic lipid [13,26] carrying Mycobacterium tuberculosis [13,27], Chlamydia trachomatis [11], or Neisseria meningitides antigens enhanced the cellular and humoral immune re...

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Section: Immunoadjuvant Properties Of Pdda/ova Npsmentioning

confidence: 99%

Simple Nanoparticles from the Assembly of Cationic Polymer and Antigen as Immunoadjuvants

et al. 2020

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“…The PMMA/CTAB or PMMA/DODAB hybrid and polymeric NPs were obtained by emulsion polymerization of MMA using AIBN as the initiator of the MMA polymerization, similar to the synthesis previously described using potassium persulfate (KPS) as the initiator [27]. The synthesis protocol was similar to the one previously described for the synthesis of PMMA/PDDA NPs using AIBN as the initiator [21][22][23]. In order to eliminate oxygen, a flow of nitrogen gas was applied for a few minutes to 10 mL DODAB or CTAB dispersions in 1 mM NaCl added of the desired MMA concentration and 0.0036 g AIBN.…”

Section: Synthesis Of Waterborne Pmma/qacs Nanoparticles (Nps) By Emumentioning

confidence: 99%

“…Some examples of biocompatible polymers are poly (lactic-co-glycolic acid) [9], poly (ε-caprolactone) (PCL) [10], poly (lactic acid) [11], poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) [12], chitosan [13,14], cellulose [15], and poly (acrylates) including poly (methyl methacrylate) (PMMA) [6,[16][17][18]. Among the poly (acrylates), several cationic latexes and coatings obtained from additives such as cationic surfactants, lipids, antimicrobial polymers or co-polymers have been described [19][20][21][22][23][24][25]. In the case of PMMA, the discovery of PMMA biocompatibility occurred when an optical technician placed a PMMA scleral lens on his eye and found that it could be well tolerated.…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanoparticles of Poly (Methyl Methacrylate) and Antimicrobial Quaternary Ammonium Surfactants

Mathiazzi

Carmona-Ribeiro

2020

Pharmaceutics

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Quaternary ammonium surfactants (QACs) are microbicides, whereas poly (acrylates) are biocompatible polymers. Here, the physical and antimicrobial properties of two QACs, cetyl trimethyl ammonium bromide (CTAB) or dioctadecyl dimethyl ammonium bromide (DODAB) in poly (methyl methacrylate) (PMMA) nanoparticles (NPs) are compared to those of QACs alone. Methyl methacrylate (MMA) polymerization using DODAB or CTAB as emulsifiers and initiator azobisisobutyronitrile (AIBN) yielded cationic, nanometric, homodisperse, and stable NPs. NPs’ physical and antimicrobial properties were assessed from dynamic light scattering (DLS), scanning electron microscopy, and viability curves of Escherichia coli, Staphylococcus aureus, or Candida albicans determined as log(colony-forming unities counting) over a range of [QACs]. NPs were spherical and homodisperse but activity for free QACs was higher than those for QACs in NPs. Inhibition halos against bacteria and yeast were observed only for free or incorporated CTAB in NPs because PMMA/CTAB NPs controlled the CTAB release. DODAB displayed fungicidal activity against C. albicans since DODAB bilayer disks could penetrate the outer glycoproteins fungus layer. The physical properties and stability of the cationic NPs highlighted their potential to combine with other bioactive molecules for further applications in drug and vaccine delivery.

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“…Cationic lipid polymer NPs and coatings are strategic for applications in antimicrobial biomimetics and vaccine design [7,8,[38][39][40][41][42][43][44][45][55][56][57][58][59]. Due to the cationic lipid, lipid polymer cationic NPs can combine effectively with antibiotics DOI: http://dx.doi.org/10.5772/intechopen.84618 like amphotericin B or miconazole [39,[68][69][70], rifampicin [71], clarithromycin [72], anti-inflammatory hydrophobic drugs like indomethacin [32], nucleic acids [21], oligonucleotides [35,73,74], proteins in general including serum proteins, transmembrane proteins, receptors and toxins [75][76][77][78], antigens [9,20,22,[34][35][36][37], peptides [23,[43][44][45] or polymers [79,80].…”

Section: Cationic Lipid Polymer Nanoparticles Coatings and Applicatimentioning

confidence: 99%

“…This was a facile alternative approach in comparison to strategies based on the synthesis of block copolymers incorporating both functions. Figure 3 shows the macroscopic and microscopic appearance of the very stable dispersions of PMMA/ PDDA and PMMA/PDDA/cationic amphiphile and some of their films obtained Biomimetic Nanomaterials from the Assembly of Polymers, Lipids, and Surfactants DOI: http://dx.doi.org/10.5772/intechopen.84618 by casting and drying the water dispersions of NPs onto different surfaces such as polystyrene, silicon wafers or glass coverslips [80].…”

Section: Cationic Lipid Polymer Nanoparticles Coatings and Applicatimentioning

confidence: 99%

Biomimetic Nanomaterials from the Assembly of Polymers, Lipids, and Surfactants

Carmona-Ribeiro¹

2019

Surfactants and Detergents

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Nanostructured materials require evaluation at a molecular level to become controllable and useful in drug and vaccine delivery. Over the years self-assembled nanomaterials such as nanoparticles and thin films have been prepared, characterized and used for biomedical applications. In this review meaningful examples of biomimetic nanomaterials and their construction based on intermolecular interactions such as the electrostatic attraction or the hydrophobic effect will be discussed. Emphasis will be placed on the interactions between polymers, lipids, surfactants and surfaces leading to bioactive supramolecular assemblies such as nanoparticles and coatings. Among the important applications of the self-assembled nanostructures and films to be reviewed are their antimicrobial effect and their adjuvant activity for vaccine delivery.

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Antimicrobial Coatings from Hybrid Nanoparticles of Biocompatible and Antimicrobial Polymers

Cited by 39 publications

References 40 publications

Simple Nanoparticles from the Assembly of Cationic Polymer and Antigen as Immunoadjuvants

Simple Nanoparticles from the Assembly of Cationic Polymer and Antigen as Immunoadjuvants

Hybrid Nanoparticles of Poly (Methyl Methacrylate) and Antimicrobial Quaternary Ammonium Surfactants

Biomimetic Nanomaterials from the Assembly of Polymers, Lipids, and Surfactants

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