Antimicrobial Drug Efflux Pumps in Stenotrophomonas maltophilia

Sánchez, María Blanca; García-León, Guillermo; Hernandez, Alvaro G.; Martínez, José Luis

doi:10.1007/978-3-319-39658-3_15

Cited by 4 publications

(7 citation statements)

References 80 publications

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“…Without considering the strain and effect (downregulation or overexpression), we observed that EtOH affected the expression of all the promoters analyzed, and CLX and MEM at least three out of the four RND members studied, including the regulatory system and two efflux pumps. In line with this, it was previously reported that the same compound can be exported by several pumps, since efflux pumps represent an important mechanism of resistance as they can expel different compounds with varied structures (Sánchez Díaz, 2003).…”

Section: Discussionmentioning

confidence: 59%

“…Although several mechanisms of antimicrobial resistance have been described for A. baumannii (Vila et al, 2007), efflux pumps are of special interest. Firstly, the primitive function of these secretion systems was the removal of metabolic end products or the expulsion of toxins or quorum-sensing molecules (Helling et al, 2002;Sánchez Díaz, 2003), suggesting that these transporters had a different biological function and were later adapted to the expulsion of antibiotics. This confers a non-specificity of substrate and may be related to their ability to actively export multiple, structurally-distinct classes of antimicrobials (Poole, 2002;Sánchez Díaz, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Firstly, the primitive function of these secretion systems was the removal of metabolic end products or the expulsion of toxins or quorum-sensing molecules (Helling et al, 2002;Sánchez Díaz, 2003), suggesting that these transporters had a different biological function and were later adapted to the expulsion of antibiotics. This confers a non-specificity of substrate and may be related to their ability to actively export multiple, structurally-distinct classes of antimicrobials (Poole, 2002;Sánchez Díaz, 2003). Secondly, these efflux pumps are widely distributed as they have been identified in several microorganisms (Nikaido, 1994) and the presence of several genes coding for different pumps in bacterial genomes have been reported.…”

Section: Introductionmentioning

confidence: 99%

“…They thus represent an important intrinsic resistance mechanism. Importantly, efflux pump overexpression has been associated with acquired resistance (Nikaido, 1994;Sánchez Díaz, 2003), which favors the emergence of mutants with a high degree of resistance (Sánchez Díaz, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, six families of multidrug efflux systems have been associated with multidrug resistance in bacteria (Sánchez Díaz, 2003;Vila et al, 2007;Opazo et al, 2009), and at least five of them have been found in the genome of A. baumannii (Coyne et al, 2011). Among them, overexpression of the resistance-nodulation-division (RND) family, which includes pumps that are proton antiporters, has been associated with the development of multidrug-resistance in this species (Yoon et al, 2013;Salehi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Subinhibitory Concentrations of Clinically-Relevant Antimicrobials Affect Resistance-Nodulation-Division Family Promoter Activity in Acinetobacter baumannii

et al. 2021

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Efflux pumps contribute to multidrug resistance in Acinetobacter baumannii due to their ability to expel a wide variety of structurally unrelated compounds. This study aimed to characterize the effect of subinhibitory concentrations of clinically-relevant antibiotics and disinfectants on the promoter activity of members of the Resistance-Nodulation-Division (RND) family in A. baumannii. The promoter regions from three RND efflux pumps (AdeABC, AdeFGH and AdeIJK) and the AdeRS regulatory system from three different A. baumannii strains (ATCC 17961, ATCC 17978, and ATCC 19606) were cloned into a luciferase reporter system (pLPV1Z). Promoter activity was quantitatively assessed in both exponential and stationary phase cultures after exposure to subinhibitory concentrations of four antibiotics from different classes (rifampicin, meropenem, tigecycline and colistin) and two disinfectants (ethanol and chlorhexidine). Subinhibitory concentrations of the compounds tested had variable effects on promoter activity that were highly dependent on the A. baumannii strain, the compound tested and the growth phase. Fold changes in AdeABC promoter activity ranged from 1.97 to 113.7, in AdeFGH from −5.6 to 1.13, in AdeIJK from −2.5 to 2, and in AdeRS from −36.2 to −1.32. Taken together, these results indicate that subinhibitory concentrations of clinically-relevant antibiotics and disinfectants affect the promoter activity of RND family members in A. baumannii in a strain and growth phase dependent manner. These results may have important implications for the treatment of infections caused by A. baumannii.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Subinhibitory Concentrations of Clinically-Relevant Antimicrobials Affect Resistance-Nodulation-Division Family Promoter Activity in Acinetobacter baumannii

et al. 2021

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Relationship between antibiotic resistance with class 1 integron and SmeDEF efflux pump encoding genes in clinical isolates of Stenotrophomonas maltophilia

Bafandeh Zamanpour,

Yousefi Mashouf,

Salimizand

et al. 2023

J Appl Genetics

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Stenotrophomonas maltophilia is an emerging multidrug-resistant organism with an increasing frequency of hospital-acquired infections predominantly in developing countries. The purpose of this study was to determine the antibiotic resistance and frequency of the smeD, class1 integron, and sul1 genes in clinical isolates of S. maltophilia in two Iranian provinces. From January 2020 to September 2021, 38 clinical isolates of S. maltophila were collected from patients in hospitals in Tabriz and Sanandaj provinces of Iran. S. maltophilia isolates were con rmed by standard bacteriological tests and 16S rRNA gene PCR. Disc diffusion and E-test methods were used to determine the antibiotic resistance pattern. PCR was performed to investigate the presence of smeD, class1 integron, and sul1 genes. The antimicrobial test for the isolated S. maltophilia showed a high level of sensitivity against most of the antibiotics used. Maximum sensitivity was recorded for cipro oxacin [100% (38/38)] and levo oxacin 100% (38/38), followed by ceftazidim [97.36% (37/38)], trimethoprim-sulfamethoxazole [94.73% (36/38)], ticarcillinclavulanate [60.52% (23/38)] and piperacillin-tazobactam [55.26% (21/38)]. We observed a high prevalence of smeD [100% (38/38)] and class1 integron [94.73% (36/38)] genes in the isolates, and none of the isolates carried the sul1 gene. The ndings from this study indicate that resistance to trimethoprimsulfamethoxazole was not observed and still, trimethoprim-sulfamethoxazole is the best drug with desirable antimicrobial effect in the treatment of nosocomial infections caused by S. maltophilia strains. Despite the observation of a high number of class1 integron, the sul1 gene was not observed, which indicates the role of this gene in high-level trimethoprim-sulfamethoxazole resistance and not having a role in low-level resistance. Based on our results clinical microbiology laboratories need continuous surveillance of resistance rates to trimethoprim-sulfamethoxazole, because of the possibility of S. maltophilia acquiring trimethoprim-sulfamethoxazole-resistance by mobile gen elements.

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Improved mini-Tn 7 Delivery Plasmids for Fluorescent Labeling of Stenotrophomonas maltophilia

Mamat,

Hein,

Grella

et al. 2023

Appl Environ Microbiol

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Antimicrobial Drug Efflux Pumps in Stenotrophomonas maltophilia

Cited by 4 publications

References 80 publications

Subinhibitory Concentrations of Clinically-Relevant Antimicrobials Affect Resistance-Nodulation-Division Family Promoter Activity in Acinetobacter baumannii

Subinhibitory Concentrations of Clinically-Relevant Antimicrobials Affect Resistance-Nodulation-Division Family Promoter Activity in Acinetobacter baumannii

Relationship between antibiotic resistance with class 1 integron and SmeDEF efflux pump encoding genes in clinical isolates of Stenotrophomonas maltophilia

Improved mini-Tn 7 Delivery Plasmids for Fluorescent Labeling of Stenotrophomonas maltophilia

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