Antimicrobial Effect of Aqueous and Ethanolic Extracts of Satureja Bachtiarica on Some Pathogenic Bacteria in Vitro

Abstract

Background: Essences and extracts of herbs, possess a variety level of biological activities, in addition the antimicrobial activities of a large number of these have been proved. With regard to the biologically active compounds and traditional use of the Satureja bachtiarica, it seems that this plant has significant antimicrobial effects. Objectives: The aim of this study is to evaluate the antimicrobial effect of aqueous and ethanolic extracts of S. bachtiarica against Enterobacter aerogenes, Bacillus subtilis and Listeria innocua of the important food pathogen. Materials and Methods:In this experimental study, antimicrobial effect of extracts was evaluated by two methods, Collins method and disk agar diffusion method on Enterobacter aerogenes ATTC 13048, Listeria innocua ATTC 33090 and Bacillus subtilis PTCC 1720. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determined using a dilution method. Statistical analysis was carried out by analysis of variance (ANOVA) technique. Results: All ethanolic extract concentrations had an inhibitory effect on the disk agar diffusion method. In Collins method ethanolic extract, prevented the growth of both strains on medium. The MBC of ethanolic extract of S. bachtiarica for B. subtilis and L. innocua was 16 and 32 mg/mL respectively, and for E. aerogenes was 64 mg/mL. But the MBC of aqueous extract of S. bachtiarica for B. subtilis and L. innocua was 32 and 64 mg/mL, respectively and for E. aerogenes was 128 mg/mL. Conclusions: The S. bachtiarica extract showed the more effective impact on the growth of B. subtilis PTCC 1720 and L. innocua ATTC 33090 than E. aerogenes ATTC 13048 (P < 0.05). The results indicated that ethanolic extract of S. bachtiarica had maximum effect on Gram positive bacteria B. subtilis PTCC 1720 and L. innocua ATTC 33090.

Effect of Salvia chorassanica Root Aqueous, Ethanolic and Hydro Alcoholic Extracts on Staphylococcus aureus, Enterococcus faecalis, Salmonella typhimurium and Escherichia coli

“…These results showed that aqueous extract had the inhibitory effect on Enterococcus faecalis merely in 480 and 960 mg/mL concentrations. Similarly, Heidari-Sureshjani et al [17] found aqueous extract of S. bachtiarica at 60 and 80 mg/mL had the inhibitory effect on Enterobacter aerogenes, however, 20 and 40 mg/mL have no significant antimicrobial effect on E. aerogenes. These findings may be due to the choice of extraction method which is applied for extracting the active compounds.…”

“…Heidari Sureshjani et al 2015 [17] also showed that ethanolic extract of Salvia chorassanica had exhibited broad spectrum activity against Enterobacter aerogenes, Listeria innocua and Bacillus subtilis as compared to aqueous extract. Previous chemical investigations on different species of salvia have shown the presence of flavonoids, diterpenoids, triterpenoids, sesterterpenes, and essential oils exhibits the antitumor, antimicrobial, cytotoxic and anti-inflammatory activities [23][24][25].…”

Antimicrobial and anti-aflatoxigenic activities of nanoemulsions based on Achillea millefolium and Crocus sativus flower extracts as green promising agents for food preservatives

“…Previous studies pointed out the impact of natural extract as an antimicrobial agent against harmful microorganisms [ 46 ]. Numerous published research on the antibacterial activity of nanoemulsions against Gram-positive and Gram-negative bacteria [ 47 , 48 ], revealed that nanoemulsions had very little antibacterial impact on gram-positive bacteria.…”

Anti-microbial Properties of Valeriana officinalis, Satureja bachtiarica and Thymus daenensis Methanolic Extracts against Helicobacter pylori

“…[31]. In this regard, another study showed that the MBC of Satureja bachtiarica ethanol extract for Bacillus subtilis and Listeria innocua were 16 and 32 mg/ml, respectively [32]. In a similar study, the extract of this plant at 2 mg / ml concentration had an inhibition effect against Streptococcus pyogenes and Staphylococcus epidermidisat but it did not effect on Pseudomonas aeruginosa [33].…”