Antimicrobial Effect of Piceatannol, a Resveratrol Metabolite, on Staphylococcus Aureus

Abstract

Objectives: Staphylococcus aureus (S. aureus) is one of the major human pathogens in both community acquired and nosocomial infections. Heavy increase of antibiotic resistance between S. aureus strains became an important public health problem in progress of time. In this study, the antimicrobial effects of piceatannol on S. aureus growth was investigated. Patients and Methods: The antimicrobial effect of piceatannol on a standard S. aureus (DSMZ 6148) strain and two clinical S. aureus strains (C1 and C2) was tested in vitro at concentrations between 0 and 750 µg/ mL. Tigecycline and gentamicin were used as positive controls. For each strain, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of piceatannol and the control antibiotics were determined separately using the broth microdilution method according to CLSI (Clinical and Laboratory Standards Institute) standards at 24 and 48 h. Results: After 24 and 48 h of treatment with piceatannol, the average MIC for all tested strains was 283 µg/mL and 383 µg/mL, respectively. Bactericidal activity increased as piceatannol concentration increased for one of the three strains. After 24 and 48 h of treatment with piceatannol, the average MBC for all strains was 717 µg/ mL and 583 µg/ mL, respectively. The S. aureus strains were found to be susceptible to tigecycline and gentamicin. Conclusion: Piceatannol has antimicrobial effect against S. aureus; however, more data regarding the effects of this compound on other microorganisms and its bioavailability are needed.

Mechanism of Synergy between Piceatannol and Ciprofloxacin against Staphylococcus aureus

“…Among them, J-28 was a multidrug-resistant bacterium. In addition, we determined the MIC of PIC against S. aureus to be 64∼128 µg/mL, which is different from that of a previous study [21]. In analyzing the results of the MIC and MBC, we found no difference in the antibacterial activity of PIC against drug-resistant and sensitive bacteria.…”

“…Moreover, PIC also exhibits anti-inflammatory, anti-adipogenesis, and anti-melanin production activities [ 19 ]. Most importantly, PIC can inhibit the biofilm of Streptococcus mutants and exhibits antibacterial activity against S. aureus [ 20 , 21 ].…”

Exploring the therapeutic potential of naturally occurring piceatannol in non‐communicable diseases

“…Guldas et al demonstrated the antimicrobial effect of PIC on standard strains of S. aureus and two clinical strains of S. aureus (C1 and C2), tested in vitro at different concentrations between 0 and 750 μg/mL. 64 The findings of the in vitro study exhibited that after 24 and 48 h of PIC treatment, the average minimum inhibitory concentrations (MIC) for all strains tested were 283 and 383 μg/mL, as well as the average minimum bactericidal concentrations, which were 717 and 583 μg/mL. Inhibition of S. mutans biofilm via inhibition of glucosyltransferase (Gtfs) using PIC was described by Nizampatanam et al 65 It was observed that PIC exhibited low micromolar antibiofilm activity with a selectivity index of around 11 by inhibiting Gtfs, a major virulence factor in S. mutans.…”

“…From the animal study, it was observed that PIC significantly reduces the formation of bacterial colonies and inflammatory responses in target organs. Guldas et al demonstrated the antimicrobial effect of PIC on standard strains of S. aureus and two clinical strains of S. aureus (C1 and C2), tested in vitro at different concentrations between 0 and 750 μg/mL 64 . The findings of the in vitro study exhibited that after 24 and 48 h of PIC treatment, the average minimum inhibitory concentrations (MIC) for all strains tested were 283 and 383 μg/mL, as well as the average minimum bactericidal concentrations, which were 717 and 583 μg/mL.…”