Antimicrobial effect of platelet-rich plasma and platelet-rich fibrin

Badade, Pallavi; Mahale, Swapna; Panjwani, Alisha A; Vaidya, Prutha; Warang, Ayushya

doi:10.4103/0970-9290.186231

Cited by 71 publications

(72 citation statements)

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“…As expected, growth at RT was significantly greater than that observed in CS samples, with growth in CS samples largely static throughout. Interestingly, despite reports of antimicrobial activity associated with PLT-rich plasma, [19][20][21][22] no obvious antimicrobial activity was observed in either RT or CS samples. Because metabolic activity is reduced with refrigeration, we sought to determine if metabolic by-products may contribute to bacterial growth as well.…”

Section: Bacterial Growthmentioning

confidence: 85%

Platelet enhancement of bacterial growth during room temperature storage: mitigation through refrigeration

et al. 2019

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INTRODUCTION Due to high risk of septic transfusion reactions arising from bacterial contamination, US Food and Drug Administration regulations currently limit platelet storage to 5 days at room temperature (RT). However, blood culturing methods can take up to 7 days to detect bacteria, allowing transfusion of potentially contaminated units. Thus, cold storage (CS) may be a viable means of extending shelf life and improving safety. STUDY DESIGN AND METHODS Platelets and fresh plasma (FP) were collected by apheresis from healthy donors, aliquoted, and challenged with Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, or Staphylococcus epidermidis. Aliquots were then stored at either RT or CS. RESULTS Significant (p < 0.05) bacterial growth was detected at RT for most bacteria as early as Day 1 after collection, with peak growth occurring between Days 3 and 4. Growth remained static during CS. Additionally, platelets appeared to enhance bacterial replication with growth significantly lower (p < 0.05) in FP relative to RT‐stored platelets. Lactic acid promoted bacterial growth when added to FP at RT. Bacterial challenge also resulted in significantly increased platelet activation (p < 0.05) and significantly reduced platelet function (p < 0.05) in RT storage relative to uninfected controls by Day 5 after collection. Conversely, CS ablated bacteria growth, limited platelet metabolism, and preserved platelet function throughout the study. CONCLUSION These data suggest that CS presents an attractive alternative to RT to both extend storage life and reduce the risk of transfusion‐related sepsis.

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Section: Bacterial Growthmentioning

confidence: 85%

Platelet enhancement of bacterial growth during room temperature storage: mitigation through refrigeration

et al. 2019

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“…[242526] The failure in our study for PRP to show superior regeneration compared to OFD may be because of conducive environment for the above described complicated time dependent periodontal wound healing phenomena. [2427282930] Comparing percentage of defect fill between Group A and Group B - it was not found to be statistically significant.…”

Section: Discussionmentioning

confidence: 96%

Use of platelet rich plasma in the management of periodontal intra-osseous defects: A clinical study

Jalaluddin

Singh

Jayanti

et al. 2017

J Int Soc Prevent Communit Dent

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Background:Periodontal disease is characterized by the presence of gingival inflammation, periodontal pocket formation, loss of connective tissue attachment, and alveolar bone around the affected tooth. Alveolar bone support and attachment apparatus regeneration has been achieved through various processes and have given elusive results. An expedient and cost-effective approach to obtain autologous platelet-derived growth factor (PDGF) and transforming growth factor (TGF)-β is the use of platelet-rich plasma (PRP). PRP is obtained by sequestrating and concentrating platelets by gradient density centrifugation.Aims:The current study was aimed at evaluating the regenerative potential of platelet-rich plasma in comparison with open flap debridement.Settings and Designs:This study was a randomized controlled clinical trial conducted in the Department of Periodontics and Oral Implantology, KIDS, Bhubaneswar, Odisha.Materials and Methods:Twenty periodontal infrabony defects in 10 patients; 6 males and 4 females of age between 25–45 years were included in this study and were followed up for a period of 6 months.Statistical Analysis:Both the groups showed a mean plaque index of 2.10 and 2.50 at baseline, 1.75 and 2.05 at 3 months, and 1.28 and 1.53 at the end of 6 months. The mean reduction of 0.35 and 0.45 at three months and 0.82 and 0.97 at six months was achieved, which was statistically significant. (P < 0.001). When comparison was done between the two groups it was not found to be statistically significant (P < 0.05). In each of the group there was definitive reduction in plaque score over a period of time.Results and Conclusion:There was no statistically significant difference in the treatment outcome between open flap debridement and PRP alone. Platelet-rich plasma application holds promise and needs further exploration.

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“…Furthermore, antimicrobial and anti-inflammatory effects of PRF have also been described [27]. Dohan et al [28] stated that PRF has immunological and antibacterial properties due to its leukocyte degranulation, and possess some cytokines that may induce angiogenesis and pro/antiinflammatory reactions.…”

Section: Discussionmentioning

confidence: 99%

The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis

et al. 2020

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Introduction/Objective The objective of the study was to investigate whether there are differences in therapeutic effect between initial treatments of chronic periodontitis [scaling and root planning (SRP)] alone and SRP in conjunction with injectable platelet-rich fibrin (I-PRF) application, comparing clinical parameters after three months. Methods Twenty-four patients with chronic periodontitis who had at least two sites with probing pocket depth (PPD) ≥ 5 mm on contralateral side participated in the study. Using a split-mouth design, the patients were treated with SRP + I-PRF (study group) or SRP only (control group). The clinical parameters, clinical attachment level (CAL), gingival margin level (GML), PPD, bleeding on probing, and plaque index, were recorded on both sides. Results Compared to baseline, both treatment modalities demonstrated an improvement in investigated clinical parameters. The mean value of CAL was reduced from 1.97 ± 0.75 (0.25-3.31) to 1.07 ± 0.44 (0.12-1.78) in the study group, whereas it decreased from 1.81 ± 0.66 (0.42-2.96) to 1.48 ± 0.55 (0.22-2.30) in the control group. Similarly, the corresponding values for GML and PPD showed statistically significant difference between the groups (p = 0.040 and p = 0.006, respectively). Conclusion Regardless the limited number of patients in the study, initial periodontal therapy in conjunction with injectable platelet-rich fibrin proved to display significant improvement in all clinical parameters compared to initial periodontal therapy alone.

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Antimicrobial effect of platelet-rich plasma and platelet-rich fibrin

Abstract: PRP is a potentially useful substance in the fight against periodontal pathogens. This might represent a valuable property in adjunct to the enhancement of tissue regeneration.

Cited by 71 publications

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Platelet enhancement of bacterial growth during room temperature storage: mitigation through refrigeration

Platelet enhancement of bacterial growth during room temperature storage: mitigation through refrigeration

Use of platelet rich plasma in the management of periodontal intra-osseous defects: A clinical study

The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis

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