Antimicrobial effect of probiotics on bacterial species from dental plaque

Abstract

Introduction: The antimicrobial role of probiotic Lactobacillus casei subspecies casei DG (L. casei DG) and of the mix culture of probiotic Lactobacillus acidophilus LA-5 and Bifidobacterium BB-12 was tested on species of Staphylococcus, Streptococcus, Pasteurella, and Neisseria genera from supragingival sites from dogs with dental disease of different breed, age, sex, weight, and diet. The research was conducted on these four genera because of their importance in zoonotic infections after dog bites. Methodology: Species from Staphylococcus, Streptococcus, Pasteurella, and Neisseria genera were isolated and identified. To test the antimicrobial efficacy of L. casei DG and the mixed culture of probiotic L. acidophilus LA-5 and Bifidobacterium bifidum BB-12 on the pathogenic species, the agar overlay method was used. Results: L. casei DG had a bactericidal effect on all analyzed species isolated from Staphylococcus, Streptococcus, Pasteurella, and Neisseria genera after 24 hours of incubation. The mixed probiotic culture made up of L. acidophilus LA-5 and Bifidobacterium BB-12 species had no bactericidal effect on the species of Staphylococcus and Streptococcus genera, which were resistant. However, it had a bacteriostatic effect on several species of Pasteurella and Neisseria genera. Conclusions: This work highlights the antimicrobial potential of probiotics in vitro, demonstrating that the probiotic L. casei DG has a bactericidal effect on all analyzed species isolated from dental plaque and that the mix culture of probiotic L. acidophilus LA-5 and Bifidobacterium BB-12 has only a bacteriostatic effect.

Cultivable Microbiota in Mitis Salivarius Agar from Dental Plaque of Dogs

“…This was confirmed in the present study, which verified the presence of the genera Staphylococcus, Streptococcus, Actinomyces and Neisseria (as shown in Figure 1). As expected, the genera Porphyromonas, Fusobacterium and Veillonella, found in other works [19,22,23,24] were not isolated as result of the methodology applied and the nutritional and strict anaerobic needs of these bacteria.…”

“…The sequence analyses and literature review indicated that 59/179 (33%) of the bacteria isolated from dogs in this study are not normally found in human oral cavities [26,27]. Only 5/15 (33.3%) of the identified groups (14 genera plus Bisgaard taxon 16), namely Actinomyces, Neisseria, Haemophilus, Streptococcus and Lactobacillus, are considered members of human dental supragingival plaque [22,23].…”

“…Bacteria of the genera Actinomyces, Porphyromonas, Fusobacterium, Neisseria, Staphylococcus, Streptococcus and Veillonella were isolated in most samples analyzed by Dent and Marsh [19], Kim et al [22], Zambori et al [23] and Jeussete et al [24], suggesting that representatives of these genera can be considered as characteristic or indigenous members of the microbial community in dental plaque. This was confirmed in the present study, which verified the presence of the genera Staphylococcus, Streptococcus, Actinomyces and Neisseria (as shown in Figure 1).…”

“…Recent works [23,[28][29][30] presented the sensitivity profile of some isolates presented in this work when challenged by natural compounds from vegetable and other bacteria, with important findings in the study of dental plaque control in dogs. These studies have to be conducted to confirm these alternative therapic drugs to replace chlorhexidine use, that presents a set of side effects on its long-term use [5].…”

Microbial biofilm in human health - an updated theoretical and practical insight

“…Zambori et al (64) have highlighted the antimicrobial potential of probiotics in vitro. More specifically, it was demonstrated that Lactobacillus casei, subsp.…”