Antimicrobial Effects of Calcium Hydroxide and Chlorhexidine on Enterococcus faecalis

Delgado, Ronan Jacques Rezende; Gasparoto, Thaís Helena; Sipert, Carla Renata; Pinheiro, Claudia Ramos; Moraes, Ivaldo Gomes de; Garcia, Roberto Brandão; Bramante, Clóvis Monteiro; Campanelli, Ana Paula; Bernardineli, Norberti

doi:10.1016/j.joen.2010.04.013

Cited by 73 publications

(86 citation statements)

References 33 publications

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“…CH is widely used as intracanal medication due to its organic tissue dissolution potential 26) , antibacterial activity 27) , and antiinflammatory capacity 28) . Since CH is ineffective in elimination of some microorganisms such as E. faecalis, to improve its antibacterial activity, it has been combined with other materials [29][30][31][32][33][34][35][36][37][38] . In the present study, to evaluate the combination potential of CH, it was combined with two established endodontic irrigants (CHX, NaOCl) and COL a medication with possible antibacterial potentials.…”

Section: Discussionmentioning

confidence: 99%

“…This model provides reconstruction of the microanatomy of dentin, simulates the chemical environment of the root canal system, and allows biofilm development 40) . Numerous studies have evaluated the antibacterial effect of CH alone or in combination with different materials using the dentin block model [30][31][32][33][34][35][36][37][38] . However, there is still controversy on whether combination of CH with other active agents enhances its antibacterial potential.…”

Section: Discussionmentioning

confidence: 99%

“…This strain easily penetrates into dentinal tubules 41) . Since this bacterium is highly resistant against different antibacterial agents, it has been used to evaluate the antibacterial effectiveness of different intracanal medications in various studies 20,[30][31][32][34][35][36][37]43) . Accordingly, this strain was selected as a measure for antibacterial property of selected medications in the present investigation.…”

Section: Discussionmentioning

confidence: 99%

“…These results can be due to the similar pH values of CH solutions when combined with other irrigants or medications (Table 2). In contrast, many studies lend support to the CH combination procedures [31][32][33][34]38) . According to these studies, this improved antibacterial effectiveness might be due to the additive or synergistic effect of CH mixed with selected materials.…”

Section: Discussionmentioning

confidence: 99%

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Antibacterial effect of triantibiotic mixture versus calcium hydroxide in combination with active agents against Enterococcus faecalis biofilm

et al. 2014

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The aim of the present study was to compare the antibacterial effect of calcium hydroxide (CH), triantibiotic mixture (TAM), and CH in combination with chlorhexidine (CHX), sodium hypochlorite (NaOCl) or colchicine (COL) against Enterococcus faecalis (E. faecalis) in surface and deep dentinal tubules. Seventy five fresh single-rooted human teeth were infected and divided into five experimental groups (n=15). The experimental groups were treated with CH+distilled water, CH+CHX, CH+NaOCl, CH+COL+distilled water and TAM+distilled water. Dentin chips obtained from surface and deep dentin of these root canals were prepared and analyzed by counting the number of colony forming units. There was significant difference between groups in the surface dentin (p<0.05). TAM showed higher antibacterial activity compared to CH-containing groups. There was no significant difference between TAM and CH-containing groups in the deep dentin (p>0.05). CH-containing medications and TAM can be used as effective disinfectants in treatment of infected root canals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Antibacterial effect of triantibiotic mixture versus calcium hydroxide in combination with active agents against Enterococcus faecalis biofilm

et al. 2014

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“…Association of CH with chlorhexidine is indicated in primary and secondary endodontic infections, increasing antimicrobial activity against microorganisms resistant to calcium hydroxide, such as E. faecalis and C. albicans 9-11 . Delgado et al 12 using root canals contaminated with E. faecalis, showed that CH and 2% chlorhexidine association were more effective when compared with CH medication. However, other studies have not shown benefits for association with CHX 3,4,13 .…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of intracanal medications based on calcium hydroxide and zinc oxide micro- or nanoparticles: an ex vivo study

Guerreiro-Tanomaru

Aguiar

Chávez-Andrade

et al. 2017

Rev. odontol. UNESP

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Introduction Nanoparticles and associations to calcium hydroxide can be used to increase antimicrobial activity. Objective To evaluate antibacterial activity against Enterococcus faecalis and pH of intracanal medications (IM) based on calcium hydroxide (CH) and zinc oxide (ZnO) micro- or nanoparticles, and their association with 0.4% chlorhexidine (CHX). Material and method Root canals from single-rooted human teeth were inoculated and incubated for 21 days. After sample (S1), the root canals were filled with the medications for 7 days and samples were collected immediately after medication (S2) and 7 days later (S3). Counting of CFU mL-1 was performed. Polyethylene tubes filled with the medications were used for the pH evaluation after 3, 7, 14 and 28 days. Data were submitted to ANOVA and Tukey tests (p<0.05). Result All IM promoted elimination of E. faecalis immediately after medication (S2). All the pastes promoted a similar pH increase. Conclusion CH/ZnO micro- or nanoparticles associated with CHX promoted greater bacterial reduction in the root canals and similar pH.

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Response of mice connective tissue to intracanal dressings containing chlorhexidine

Pereira

Faria

Silva

et al. 2012

Microscopy Res & Technique

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Substances containing chlorhexidine (CHX) have been studied as intracanal medicaments. The aim of the present study was to characterize the response of mouse subcutaneous connective tissue to CHX-containing medications by conventional optical microscopy. The tissue response was evaluated by implanting polyethylene tubes containing one of the substances evaluated: Calen paste + 0.5% CHX, Calen + 2% CHX, 2% CHX gel, and Calen paste (control). After experimental periods of 7, 21, and 63 days, the implants (n = 10) were removed along with the subcutaneous connective tissue. Tissue samples were subjected to histological processing, and sections were stained with hematoxylin and eosin. Qualitative and quantitative analyses of the number of inflammatory cells, blood vessels, and vascularized areas were performed. Results were analyzed by ANOVA and Tukey tests with the significance level set at 5%. We concluded that Calen + 0.5% CHX led to reparative tissue response in contrast with Calen + 2% CHX and 2% CHX gel, which induced persistent inflammatory response, pointing to the aggressive nature of this mixture. When Calen + 2% CHX and 2% CHX gel were compared, the latter induced more intense inflammatory response.

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Antimicrobial Effects of Calcium Hydroxide and Chlorhexidine on Enterococcus faecalis

Cited by 73 publications

References 33 publications

Antibacterial effect of triantibiotic mixture versus calcium hydroxide in combination with active agents against Enterococcus faecalis biofilm

Antibacterial effect of triantibiotic mixture versus calcium hydroxide in combination with active agents against Enterococcus faecalis biofilm

Antibacterial activity of intracanal medications based on calcium hydroxide and zinc oxide micro- or nanoparticles: an ex vivo study

Response of mice connective tissue to intracanal dressings containing chlorhexidine

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