2023
DOI: 10.3390/ijerph20032447
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Antimicrobial Effects of Non-Thermal Atmospheric Pressure Plasma on Oral Microcosm Biofilms

Abstract: We comparatively evaluated the antibacterial effects of non-thermal atmospheric pressure plasma (NTAPP) on oral microcosm biofilms. Oral microcosm biofilms, which are derived from inoculation with human saliva, were cultured on 48 hydroxyapatite disks for 6 d. The prepared biofilms were divided into three different daily treatment groups: distilled water for 1 min, 0.12% chlorhexidine for 1 min, and NTAPP for 5 min. Using a quantitative light-induced fluorescence-digital camera, the red fluorescence intensity … Show more

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Cited by 4 publications
(9 citation statements)
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“…Oral microcosm biofilms were allocated into six groups based on the biofilms' treatment and maturity: Groups 1 and 2 were treated with 1.5 mL of distilled water (DW) for 1 min, serving as the negative control for 2-day and 6-day biofilms, respectively. Groups 3 and 4 received CAP treatment with an Ar-gas-based plasma pipette (Femto Science, Hwaseong, Republic of Korea) operated at a power setting of 4 W, a voltage of 10 kV, and a frequency of 100 kHz (Figure 1A) [21]. CAP treatment was administered for 2 min, and the output pressure of the regulator was adjusted to 0.02 MPa; the plasma column temperature was maintained at 40 • C, and a consistent distance of 10 mm was maintained between the top of the handpiece nozzle and the biofilm surface (Figure 1B).…”
Section: Group Allocation and Treatmentmentioning
confidence: 99%
“…Oral microcosm biofilms were allocated into six groups based on the biofilms' treatment and maturity: Groups 1 and 2 were treated with 1.5 mL of distilled water (DW) for 1 min, serving as the negative control for 2-day and 6-day biofilms, respectively. Groups 3 and 4 received CAP treatment with an Ar-gas-based plasma pipette (Femto Science, Hwaseong, Republic of Korea) operated at a power setting of 4 W, a voltage of 10 kV, and a frequency of 100 kHz (Figure 1A) [21]. CAP treatment was administered for 2 min, and the output pressure of the regulator was adjusted to 0.02 MPa; the plasma column temperature was maintained at 40 • C, and a consistent distance of 10 mm was maintained between the top of the handpiece nozzle and the biofilm surface (Figure 1B).…”
Section: Group Allocation and Treatmentmentioning
confidence: 99%
“…Fifty-seven hydroxyapatite (HAP) disks (Himed Inc., Old Bethpage, NY, USA), each measuring 7 mm in diameter and 2 mm in height, were prepared [22]. The disk surfaces were ground using sandpaper (400-1200 grit) and a polishing machine (M-PREP 5TM; Allied High Tech Products, Inc., Compton, CA, USA) (Figures 1 and 2A).…”
Section: Establishing the Number Of Samples And Preparation Of Specimensmentioning
confidence: 99%
“…The disk surfaces were ground using sandpaper (400-1200 grit) and a polishing machine (M-PREP 5TM; Allied High Tech Products, Inc., Compton, CA, USA) (Figures 1 and 2A). Each HAP disk was positioned within an acrylic mold in accordance with the protocols outlined in a previous study [22], ensuring that a space of 1 mm was maintained above the disk for biofilm formation (Figure 2B). Following their preparation, the specimens underwent sterilization with ethylene oxide gas.…”
Section: Establishing the Number Of Samples And Preparation Of Specimensmentioning
confidence: 99%
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“…CAP is found to be effective against a wide range of muti-drug-resistant bacteria and biofilms [16,[42][43][44]. There are several mechanisms by which CAP induces antimicrobial effect [45].…”
Section: Possible Synergies Between Cap and Psm Therapiesmentioning
confidence: 99%