Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression

Bordini, Ester Alves Ferreira; Tonon, Caroline Coradi; Francisconi, Renata Serignoli; Magalhães, Fernando Augusto Cintra; Maquera-Huacho, Patricia Milagros; Bedran, Telma Lombardo; Pratavieira, Sebastião; Spolidório, Luís Carlos; Spolidório, Denise Madalena Palomari

doi:10.1080/08927014.2018.1504926

Cited by 37 publications

(26 citation statements)

References 54 publications

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“…In this context, the efficacy of the combination of terpinen-4-ol and nystatin has been demonstrated in vitro by Tonon et al 36 , who demonstrated the importance of this compound against Candida species. However, several studies have shown that terpinen-4-ol inhibits bacterial growth and adhesion to dental biofilm 12,13,17 . A previous study reported that terpinen-4-ol 0.24% and 0.95% exhibited antimicrobial effects against S. mutans and L. acidophilus in both planktonic and biofilm forms 12 .…”

Section: Discussionmentioning

confidence: 99%

“…However, several studies have shown that terpinen-4-ol inhibits bacterial growth and adhesion to dental biofilm 12,13,17 . A previous study reported that terpinen-4-ol 0.24% and 0.95% exhibited antimicrobial effects against S. mutans and L. acidophilus in both planktonic and biofilm forms 12 . Similarly, Bucci et al 13 observed the antiadhesion and antibiofilm properties of the mixture of surfactin and terpinen-4-ol against S. mutans, P. gingivalis, and C. albicans.…”

Section: Discussionmentioning

confidence: 99%

“…Terpinen-4-ol is the main active compound in TTO and has gained attention for its antimicrobial, antifungal, and anti-inflammatory properties 11 . Terpinen-4-ol induces membrane loss to disrupt the integrity and physiology of microbial cells 12 . Several reports have demonstrated oral care products containing terpinen-4-ol have demonstrable antiseptic effects and inhibit bacterial growth and adhesion to the dental biofilm 12 , 13 .…”

Section: Introductionmentioning

confidence: 99%

“…Terpinen-4-ol induces membrane loss to disrupt the integrity and physiology of microbial cells 12 . Several reports have demonstrated oral care products containing terpinen-4-ol have demonstrable antiseptic effects and inhibit bacterial growth and adhesion to the dental biofilm 12 , 13 . Low concentrations of terpinen-4-ol do not display toxicity towards fibroblasts and epithelial cells, allowing for topical use with reduced adverse effects 14 – 17 .…”

Section: Introductionmentioning

confidence: 99%

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Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis

Francisconi

Maquera-Huacho

Tonon

et al. 2020

Sci Rep

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This study was performed to develop a liquid crystalline system (LCS) incorporated with terpinen-4-ol and nystatin to evaluate its antifungal, antibiofilm, and synergistic/modulatory activity against Candida albicans. The LCS was composed of a dispersion containing 40% propoxylated and ethoxylated cetyl alcohol, 40% oleic acid, and 0.5% chitosan dispersion. According to analysis by polarized light microscopy, rheology, and mucoadhesion studies, the incorporation of 100% artificial saliva increased the pseudoplasticity, consistency index, viscosity, and mucoadhesion of the formulation. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity; the LCS containing terpinen-4-ol and nystatin effectively inhibited C. albicans growth at a lower concentration, displaying a synergistic action. Therefore, LCS incorporated with terpinen-4-ol and nystatin is a promising alternative for preventing and treating infections and shows potential for the development of therapeutic strategies against candidiasis. Candida species are pathogenic yeasts and the leading causes of several major fungal infections in humans. These infections can be caused by increased use of immunosuppressive therapies, acquired immunodeficiency syndrome, and the emergence of drug resistance 1,2. Candida albicans is the most commonly isolated yeast species in the oral microbiota and may cause infections in the presence of other associated factors such as vertical dimension reduction, unstable occlusion, old prostheses, and roughness causing local or systemic infection 3. Among the different virulence factors, the initial adhesion resulting in biofilm formation on abiotic and biotic surfaces plays a critical role in the pathogenesis of C. albicans. Several antifungal agents are used to prevent and treat oral candidiasis, such as polyenes, ketoconazole, and miconazole 4. However, the clinical presentation of less susceptible strains leads to treatment failure, increasing disease recurrence. Several mechanisms of azole-resistant species have been reported, including those with changes in the cell wall or plasma membrane to impair azole uptake; overexpression or mutations in the target enzyme of azoles; and efflux of drugs mediated by membrane transport proteins 5. Resistance appears to increase proportionally with the extent of previous exposure to antifungal drugs. In addition, studies have shown some medications may cause injuries in the kidney and liver 6 , highlighting the need to develop new therapeutic strategies and search for agents with novel mechanisms of action that may be used independently or in combination with conventional medicines. Drug resistance is a matter of concern. The search for new agents may lead to the development of new antifungal agents that are effective against biofilms. Although the polyene nystatin is widely used to control and reduce oral fungal infections, polyenes are highly toxic to the host and their prolonged use can lead to drug res...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis

Francisconi

Maquera-Huacho

Tonon

et al. 2020

Sci Rep

Self Cite

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“…Differently, it was reported that terpinene decreased to 32.41 % at post flowering stage in A. biebersteinii . In previous studies, it was found that the volatile oil had components with antimicrobial effect such as borneol, camphor, menthone, menthol, terpenen‐4‐ol, and 1,8‐cineole …”

Section: Introductionmentioning

confidence: 88%

Evaluation of Phytochemical Compositions and Biological Properties of Achillea gypsicola at Different Phenological Stages

Açıkgöz

2019

Chemistry & Biodiversity

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Phytochemicals, which are commonly found at different levels in many medicinal plants, are natural strong antioxidants used in traditional medicine. In this research, determination of differences of phytochemical compositions and biological properties were aimed as periodically (pre-, full and post flowering) and daily (6 am, 1 pm and 8 pm) in Achillea gypsicola HUB.-MOR. The volatile oils belonging to A. gypsicola were obtained by hydrodistillation and analyzed by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC/MS). The antimicrobial activities of the volatile oils were determined with disc diffusion method. The microdilution method was used to determine minimum inhibitory concentration (MIC). Total phenolic and flavonoid contents were determined by spectrophotometric methods and antioxidant capacities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, reducing power (RP) and metal chelating activity (MCA) assay. In addition, the phenolic acid and flavonoid compositions were evaluated by reversed phase-high-performance liquid chromatography (RP-HPLC). This study presented a comprehensive report for the first time on evaluation of the phytochemical composition and the biological properties of A. gypsicola at different phenological stages. Thirty-two compounds, containing the major component as camphor, 1,8-cineole and borneol, were detected. Designated harvest time for the highest yield of volatile oils was found to be at full flowering stage-1 pm. It has been observed that the volatile oil composition changes periodically and even daily. Also, in this research, menthol and menthone were found as the composition of volatile oil in Achillea species for the first time. Full flowering stage was found as the richest period in terms of phenolic acid and flavonoid compositions of A. gypsicola for the first time. The species examined in this research showed a high antioxidant and antimicrobial activity in comparison to other studies with Achillea species. The volatile oils exhibited high performances with range of inhibition zones (8.3-42.3 mm) and minimum inhibitory concentration values (2.25-144 μg/ml). Besides, a high correlation between antioxidant activity and phenolic content of A. gypsicola was found. These results suggest that A. gypsicola can be used as a safe source in the cosmetic, food and pharmaceutical industries.

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Effects of Cuminum cyminum L. essential oil and its nanoemulsion on oxidative stability and microbial growth in mayonnaise during storage

Moradi

2023

Food Science & Nutrition

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The present study aimed to investigate the effects of Cuminum cyminum L. essential oil (CEO) and its nanoemulsion (CEON) on oxidative stability and microbial growth of mayonnaise during storage. The GC analysis indicated that Cuminaldehyde (27.99%), o‐Cymene (17.31%), γ‐Terpinen (16.67%), and β‐Pinene (9.35%) were the major components of CEO, respectively. The assessments of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) showed that Escherichia coli ATCC 25922 (MBCCEO = 12 and MBCCEON = 6 mg/mL) was the most resistant bacteria, and in contrast, Staphylococcus aureus ATCC 29213 (MBCCEO = 6 and MBCCEON = 3 mg/mL) was the most sensitive bacteria. In the radical‐scavenging assay, CEON (IC50 = 5 ± 0.07 μg/mL) exhibited a higher antioxidant activity than CEO (IC50 = 10 ± 0.13 μg/mL). The results showed that applying the MBC of CEO and CEON in mayonnaise led to a significant decrease (p < .05) in acidity, peroxide value, number of acid‐resistant bacteria and fungi, and total microbial count compared with the control sample. In conclusion, this study demonstrated that using CEON resulted in oxidative stability, microbial growth control, and desirable sensorial attributes in mayonnaise compared with CEO and control samples.

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Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression

Cited by 37 publications

References 54 publications

Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis

Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis

Evaluation of Phytochemical Compositions and Biological Properties of Achillea gypsicola at Different Phenological Stages

Effects of Cuminum cyminum L. essential oil and its nanoemulsion on oxidative stability and microbial growth in mayonnaise during storage

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