Antimicrobial Efficacy of 7-Hydroxyflavone Derived from Amycolatopsis sp. HSN-02 and Its Biocontrol Potential on Cercospora Leaf Spot Disease in Tomato Plants

Abstract

The actinomycete strain HSN-02 was isolated from the soil of a mining field in the Sandur region, Bellary, Karnataka, India. According to the morphological, cultural, physiological, and biochemical characteristics and the 16S rDNA sequence analysis, the strain HSN-02 was identified as Amycolatopsis sp. The antimicrobial activity strain HSN-02 presented stable and moderate inhibitory activity against human pathogens. In pot experiments in the greenhouse, the development of Cercospora leaf spot was markedly suppressed by treatment with the purified compound from the strain HSN-02, and the control efficacy was 45.04 ± 1.30% in Septoria lycopersici-infected tomato plants. A prominent compound was obtained from the fermentation broth of the strain HSN-02 using column chromatography and HPLC. The chemical structural analyses using UV, FTIR, HR-ESI-MS, and NMR confirmed that the compound produced by the strain HSN-02 is 7-hydroxyflavone. This investigation showed the role which the actinomycete strain can play in controlling leaf spots caused by S. lycopersici to reduce treatments with chemical fungicides.

Efficacy of Penicillium limosum Strain AK-7 Derived Bioactive Metabolites on Antimicrobial, Antioxidant, and Anticancer Activity against Human Ovarian Teratocarcinoma (PA-1) Cell Line

“…The results were similar to the earlier reports where Penicillium sclerotiorum extract treated A549, A431, U251, and HeLa cancer lines showed a significant reduction in cell viability and increased eventual cell death (apoptosis) at the IC 50 concentrations of 10, 20, 32 and 7.75 µg/mL [56]. Apoptosis can be instigated by the effective action of the drug, which causes DNA fragmentation, prevention of DNA replication, breaking of DNA double-strand (through inactivation of topoisomerase II), ROS generation (reactive oxygen species), affect the mitochondrial functioning level, and finally, damage of DNA which leads to the apoptosis [57][58][59][60]. In the present context, AK-7 extract significantly induced and influenced the apoptosis mechanism in the PA-1 cell line through a series of mechanisms that finally led to mitochondrial dysfunction and cell death compared to the untreated cell line.…”

7‐Hydroxyflavone improves nonalcoholic fatty liver disease by acting on STK24

“…Numerous types of plants include 7‐hydroxyflavone (7‐HY) (El‐Hefny et al, 2023; Roy & Pandey, 1994), which are also derived from microbes (Math et al, 2023). It has been demonstrated that 7‐HY has anti‐inflammatory (Ullah et al, 2022), antioxidant (Sengupta et al, 2017), and vasculoprotective properties (Calderone et al, 2004).…”