Antimicrobial Ellagitannin of Punica granatum Fruits

Abstract

O fracionamento do extrato acetato de etila de frutos de Punica granatum, guiado por ensaios antimicrobianos frente a colônias de Staphylococcus aureus resistentes à meticilina, conduziu ao isolamento e à determinação estrutural do tanino elágico punicalagina. A identificação da substância foi realizada por CLAE/UV e RMN de 1 H. Os ensaios antimicrobianos foram realizados pelo método de difusão em discos de papel. A concentração mínima inibitória das substâncias foi determinada pelo método de diluição em agar padronizada pelo NCCLS (National Committee for Clinical Laboratory Standards).The ethyl acetate extract of Punica granatum fruits was fractionated by chromatographic techniques to afford the ellagitannin punicalagin. The substance was found to be active against methicillin-resistant Staphylococcus aureus strains and was identified by HPLC/UV and 1 HNMR. The antibacterial assays which guided the isolation of the tannin were conducted using the disc diffusion method. Minimum inhibitory concentration (MIC) was determined by the dilution method according to NCCLS (National Committee for Clinical Laboratory Standards) procedure.

Evaluation of bioactivity of pomegranate fruit extract against Alicyclobacillus acidoterrestris DSM 3922 vegetative cells and spores in apple juice

“…At the end of 240 h, reductions of 3.46 and 3.56 log CFU/mL were obtained in the apple juice with PE at the concentrations of 20 and 40 mg/mL, respectively. In the apple juice supplemented with 2.5, 5 and 10 mg PE/mL, the cell counts were reduced from the initial count by 2.84, 3.26 and 3.32 log CFU/mL after 240 h. There are several studies showing that PE or its pure components have the antibacterial activity of against both Gramepositive and Gramenegative bacteria (Ahmad & Aqil, 2007;Burapadaja & Bunchoo, 1995;De, De, & Banerjee, 1999;Hayrapetyan et al, 2012;Machado et al, 2002;Navarro, Villarreal, Rojas, & Lozoya, 1996;Negi, Jayaprakasha, & Jena, 2003;Prashanth, Asha, & Amit, 2001;Rani & Khullar, 2004). The antimicrobial activity of plant antimicrobials depends on the type of the target organism, extraction procedure, culture medium, size of the microbial inoculum and the method used for the determination of antimicrobial activity (Tajkarimi, Ibrahim, & Cliver, 2010).…”

Pentaclethra macroloba tannins fractions active against methicillin-resistant staphylococcal and Gram-negative strains showing selective toxicity

“…The bark of this species is a rich source of condensed tannins and phenolic compounds (Schedlbauer & Kavanagh, 2008), chemical classes already recognized in the literature by exhibiting antimicrobial activities (Machado et al, 2002). Triterpenic monodesmoside saponins have already been found as major compounds in this species (Viana et al, 2004b).…”

“…P. aeruginosa has also presented increased resistance, including to imipenem, one of the antibacterial drugs with the largest spectrum of action against this pathogen . Although there is found a variety of studies considering natural products as feasible antimicrobial agents against Staphylococcus (Machado et al, 2002;Machado et al, 2005;Pereira et al, 2006;Leal et al, 2010), investigations on the inhibition of Gram-negative bacteria by these products are poorly found in the literature, awakening the interest for this investigation. So, the search for new strategies for the treatment of infections caused by these pathogens stimulates the investigation of alternative sources (Machado et al, 2005).…”

The Therapeutic Potential of Pomegranate and Its Products for Prevention of Cancer

“…However, several in vitro assays demonstrate its bacteriocidal activity against several highly pathogenic and sometimes antibiotic-resistant organisms. Machado et al (2002) evaluated the synergistic effect of a pomegranate methanolic extract with five antibiotics on 30 clinical isolates of methicilin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus. Antibiotics tested were.…”