Antimicrobial endotoxin‐neutralizing peptides promote keratinocyte migration <i>via</i> P2X7 receptor activation and accelerate wound healing <i>in vivo</i>

Pfalzgraff, Anja; Bárcena-Varela, Sergio; Heinbockel, Lena; Gutsmann, Thomas; Brandenburg, Klaus; Tejada, Guillermo Martínez de; Weindl, Günther

doi:10.1111/bph.14425

Cited by 30 publications

(25 citation statements)

References 58 publications

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“…In this study, mice in the APs-L group had significantly promoted EGF secretion on day 7, but it can also be seen that they also had a certain effect on the proliferation of CD31 and FGF compared with the negative control group (Figure 5), which was different from the results of immunohistochemistry of topical APs. This may be caused by different mechanisms of action [23], or may also be because the healing of wounds in mice rather than humans is mainly achieved by contraction rather than epithelial re-epithelialization and granulation tissue formation, resulting in differences in action [45]. This accelerated skin wound healing in mice, consistent with the results of the healing rate, epithelialization time ( Figure 1 and Table 1) and H&E (Figure 4).…”

Section: Discussionsupporting

confidence: 69%

Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds

et al. 2019

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Skin wound healing, especially chronic wound healing, is a common challenging clinical problem. It is urgent to broaden the sources of bioactive substances that can safely and efficiently promote skin wound healing. This study aimed to observe the effects of active peptides (APs) of the mantle of Pinctada martensii on wound healing. After physicochemical analysis of amino acids and mass spectrometry of APs, the effect of APs on promoting healing was studied through a whole cortex wound model on the back of mice for 18 consecutive days. The results showed that APs consisted of polypeptides with molecular weights in the range 302.17–2936.43 Da. The content of polypeptides containing 2–15 amino acids accounted for 73.87%, and the hydrophobic amino acids accounted for 56.51%. Results of in vitro experimentation showed that mice in APs-L group which were fed a low dose of APs (0.5 g/kg bw) had a shortened epithelialization time due to a shortening inflammatory period (p < 0.05). Mechanistically, this relied on its specific ability to promote the proliferation of CD31, FGF and EGF which accelerated the percentage of wound closure. Moreover, the APs-L group mice had enhanced collagen synthesis and increased type III collagen content in their wounds through a TGF-β/Smad signaling pathway (p > 0.05). Consequently, scar formation was inhibited and wound healing efficiency was significantly improved. These results show that the APs of Pinctada martensii promote dermal wound healing in mice and have tremendous potential for development and utilization in skin wound healing.

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Section: Discussionsupporting

confidence: 69%

Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds

et al. 2019

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“…AMP gene expression increased during scratch wound healing. Endogenous AMPs have also been found to be upregulated in all stages of wound healing in vivo, indicating a role beyond microbial defense toward regulation of immune response, granulation tissue formation, and re-epithelization [ 82 , 83 ]. It was also observed that antimicrobial peptide transcript levels were significantly elevated after wIRA treatment under optimal conditions, further suggesting the involvement in the improved scratch wound healing outcome.…”

Section: Discussionmentioning

confidence: 99%

Keratinocyte and Fibroblast Wound Healing In Vitro Is Repressed by Non-Optimal Conditions but the Reparative Potential Can Be Improved by Water-Filtered Infrared A

et al. 2021

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It is a general goal to improve wound healing, especially of chronic wounds. As light therapy has gained increasing attention, the positive influence on healing progression of water-filtered infrared A (wIRA), a special form of thermal radiation, has been investigated and compared to the detrimental effects of UV-B irradiation on wound closure in vitro. Models of keratinocyte and fibroblast scratches help to elucidate effects on epithelial and dermal healing. This study further used the simulation of non-optimal settings such as S. aureus infection, chronic inflammation, and anti-inflammatory conditions to determine how these affect scratch wound progression and whether wIRA treatment can improve healing. Gene expression analysis for cytokines (IL1A, IL6, CXCL8), growth (TGFB1, PDGFC) and transcription factors (NFKB1, TP53), heat shock proteins (HSP90AA1, HSPA1A, HSPD1), keratinocyte desmogleins (DSG1, DSG3), and fibroblast collagen (COL1A1, COL3A1) was performed. Keratinocyte and fibroblast wound healing under non-optimal conditions was found to be distinctly reduced in vitro. wIRA treatment could counteract the inflammatory response in infected keratinocytes as well as under chronic inflammatory conditions by decreasing pro-inflammatory cytokine gene expression and improve wound healing. In contrast, in the anti-inflammatory setting, wIRA radiation could re-initiate the acute inflammatory response necessary after injury to stimulate the regenerative processes and advance scratch closure.

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“…AMPs can promote wound healing [ 291 – 293 ] after skin injury, a process involving the complex interactions of keratinocytes, fibroblasts, vascular endothelial cells, immune cells and the extracellular matrix [ 294 – 296 ]. Some AMPs play a vital role in both skin barrier and function [ 297 ] and thus have the potential to treat multiple skin maladies, exemplified by melanoma, acne, diabetic foot ulcer and psoriasis [ 298 ].…”

Section: Activity Of Ampsmentioning

confidence: 99%

Antimicrobial peptides: mechanism of action, activity and clinical potential

et al. 2021

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The management of bacterial infections is becoming a major clinical challenge due to the rapid evolution of antibiotic resistant bacteria. As an excellent candidate to overcome antibiotic resistance, antimicrobial peptides (AMPs) that are produced from the synthetic and natural sources demonstrate a broad-spectrum antimicrobial activity with the high specificity and low toxicity. These peptides possess distinctive structures and functions by employing sophisticated mechanisms of action. This comprehensive review provides a broad overview of AMPs from the origin, structural characteristics, mechanisms of action, biological activities to clinical applications. We finally discuss the strategies to optimize and develop AMP-based treatment as the potential antimicrobial and anticancer therapeutics.

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Antimicrobial endotoxin‐neutralizing peptides promote keratinocyte migration via P2X7 receptor activation and accelerate wound healing in vivo

Abstract: Our data suggest the potential application of Pep19-2.5 in the treatment of non-infected and S. aureus-infected wounds and provide insights into the mechanism involved in Pep19-2.5-induced wound healing.

Cited by 30 publications

References 58 publications

Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds

Effect of Oral Administration of Active Peptides of Pinctada Martensii on the Repair of Skin Wounds

Keratinocyte and Fibroblast Wound Healing In Vitro Is Repressed by Non-Optimal Conditions but the Reparative Potential Can Be Improved by Water-Filtered Infrared A

Antimicrobial peptides: mechanism of action, activity and clinical potential

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