Antimicrobial Bacillus velezensis HC6: production of three kinds of lipopeptides and biocontrol potential in maize

Abstract

Aims: To study the antimicrobial agents of the Bacillus velezensis strain HC6 and assess the application potential of B. velezensis HC6 in maize. Methods and Results: We applied a dual culture technique to test the antimicrobial activity of B. velezensis HC6 against bacteria and fungi of common contaminated crops. Bacillus velezensis HC6 showed antagonistic action on pathogenic fungi, including Aspergillus and Fusarium, as well as pathogenic bacteria (especially Listeria monocytogenes). When applied in maize, B. velezensis HC6 could also inhibit the growth of multiple pathogenic fungi and reduce their production of aflatoxin and ochratoxin. Three kinds of antimicrobial lipopeptides, including iturin, fengycin and surfactin were identified in B. velezensis HC6 culture supernatant by high-performance liquid chromatography and MALDI-TOF mass spectrometry. Iturin and fengycin showed obvious antimicrobial activity to the tested fungal strains. Conclusions: Bacillus velezensis HC6 produces three kinds of lipopeptides which showed antimicrobial activity against several common pathogenic fungi and bacteria. Bacillus velezensis HC6 is potential to be biocontrol bacteria in maize. Significance and Impact of the Study: Bacillus velezensis HC6 shows obvious antimicrobial activity to important crops pathogenic fungi which usually produce mycotoxins that are harmful to animal and human health. We demonstrate that three different types of lipopeptides produced by B. velezensis contributed to the antimicrobial activity. Bacillus velezensis HC6 has the potential to be effective biocontrol agent in crops.

Screening, Identification and Efficacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae

“…B. velezensis is a new type of biocontrol factor with inhibitory ability against a broad spectrum of microbial pathogens and plant growth promoting activity [63]. It produces various antifungal and antibacterial metabolites including β-1,3-1,4-glucanase, lipopeptides (iturin, fengycin and surfactin), polyketides (macrolactin, bacillaene and difficidin or oxydifficidin) and peptides (plantazolicin, amylocyclicin and bacilysin) [64][65][66][67]. It is reported that lipopeptides produced by B. velezensis are the major contributor to inhibiting pathogenic fungi, such as F. oxysporum, C. gloeosporioides and so on [27,68].…”

Crop Protection against Botrytis cinerea by Rhizhosphere Biological Control Agent Bacillus velezensis XT1

“…Antagonistic activity against the pathogens Monilinia fructicola [ 61 ] and B. cinerea (60%) reached maximum levels of > 80% and 60%, respectively, mainly due to the large amount of lipopeptides produced (10 g/L) [ 67 ]. Many studies of olive, tomato, corn, peanut, pepper, maize and rice crop yields have also described the biocontrol activity of B. velezensis strains against phytopathogens such as Verticillium dahliae , Fusarium graminerarum , Sclerotium rolfsii , Phytophthora and B. cinerea [ 68 , 69 , 70 , 71 , 72 , 73 , 74 ]. Our results are in line with the findings of Lee et al (2006) [ 75 ] who investigated the effect of radicular applications of B. subtilis WXCDD105 on B. cinerea in tomato plants.…”

Mass Spectrometry in Clinical Laboratories

“…The versatility of HR-MS (especially hybrid HR-MS) is reflected in increased selectivity by eliminating potential interferences originating from the matrix with remarkably similar mass-to-charge ratio (m/z) as of the measured analytes, but with a different structure. Glycopeptides [20][21][22] Oxazolidinones [23,24] Ansamycins [25,26] Quinolones [27,28] Streptogramins [29,30] Lipopeptides [31,32] Antibiotic Resistance [33,34] Table 1.…”