Antimicrobial investigation of selected soil actinomycetes isolated from unexplored regions of Kashmir Himalayas, India

Shah, Aabid Manzoor; Shakeel-u-Rehman,; Hussain, Aashiq; Mushtaq, Saleem; Rather, Muzafar Ahmad; Shah, Aabid Manzoor; Ahmad, Zahoor; Khan, Inshad Ali; Bhat, Khursheed Ahmad; Hassan, Qazi Parvaiz

doi:10.1016/j.micpath.2017.06.017

Cited by 39 publications

(19 citation statements)

References 23 publications

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“…The minimum bactericidal concentration (MBC) was determined by taking a small amount of mixed liquid in a microtiter plate from the MIC test results with a loop, then scratched on Mueller-Hinton Agar (MHA; Himedia, India), and incubated for 24 hours at 37°C. All analyses used ampicillin (Oxoid, UK) as a positive control and DMSO (Merck, Germany) as the negative control [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

Kurnianto

Kusumaningrum

Lioe

2020

International Journal of Microbiology

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Streptomyces has been reported as an essential producer of bioactive substances, including antibiotics and other types of antimicrobials. This study investigated antibacterial-producing Streptomyces isolated from the gut of estuarine fish Chanos chanos, emphasizing screening for the producer of peptide-containing antibacterial compounds. Eighteen isolates were found during preliminary screening, in which four isolates showed the best antibacterial activities. Based on the morphological, physiological, and biochemical characterization, as well as 16S rRNA partial sequencing, all of the four isolates belonged to Streptomyces. Three isolates were suspected as novel isolate candidates based on homology presentations and phylogenetic tree analysis. Disk-diffusion assay of the metabolite-crude-extract from the isolates showed broad-spectrum inhibitory activities against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa InaCC B52 with minimum inhibitory concentration and minimum bactericidal concentration ranging from 2.5–10 mg/mL and 5–10 mg/mL, respectively. The highest antibacterial activity with low MIC and MBC values was shown by isolate AIA-10. Qualitative HPLC profiling revealed that the metabolic-crude-extracts showed many peaks with intensive area at 210 and 214 nm, especially from SCA-11 and AIA-10, indicating the presence of peptide groups in the structure of the constituent compound. The results also suggested that crude extracts SCA-11 and AIA-10 had higher hydrophobicity properties than the other extracts. Further characterization of the active compound was needed to find out which compounds were responsible for the antibacterial activity. The results of this study indicated that some Streptomyces isolated from new environmental niches, i.e., gut of estuarine fish Chanos chanos, produce promising peptide-containing bioactive compounds.

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Section: Methodsmentioning

confidence: 99%

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

Kurnianto

Kusumaningrum

Lioe

2020

International Journal of Microbiology

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“…Previous reports from the endophytic Streptomyces sp. obtained from tomato plant showed a signi cant antibacterial (Shah et al 2017), antifungal, activity, enhance disease resistance (Abbasi et al 2019) and plant growth promotion (Zarandi et al 2021).…”

Section: Resultsmentioning

confidence: 99%

The Draft Genome Sequence of The Endophytic Streptomyces Strain VITGV156

Veilumuthu

Nagarajan

Sasikumar

et al. 2021

Preprint

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Streptomyces species is one among the dominant group of bacteria in the family Actinobacteria with a rich repertoire of secondary metabolites. Secondary metabolites with antimicrobial activity and plant growth promotor have been isolated from various Streptomyces sp. Here in this investigation, we present the draft genome of a new species, Streptomyces sp. VITGV156 isolated from healthy tomato plant (Lycopersicon esculentum) which has some rare antimicrobial secondary metabolites, like coelichelin, fluostatins, vicenistatin, nystatin, sipanmycin, and informatipeptin. The genome is 8.18 Mb in size with 6,259 protein coding genes. The average GC content of the genome is 72.61 %. Preliminary analysis with antiSMASH 6.0 revealed the presence of 29 biosynthetic gene clusters for the synthesis of potential secondary metabolites. These includes 4 NRPS (non – ribosomal peptide synthetase), 7 PKS (Polyketide Synthases), 2 RiPP (Ribosomally synthesized and post-translationally modified peptides) clusters. When we look into genes associated with secondary metabolites, 406 genes are present which includes 184 genes for cofactor and vitamins, 72 genes for terpenoids and polyketides, 70 genes for xenobiotics and 80 genes for other metabolites are present. Comparative genome analysis of VITGV156 with its closest neighbor Streptomyces luteus strain TRM45540 revealed ANI 91.22% and dDDH value 44.00%.

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“…SM01 identified as a Streptomyces (Fig. 1a), the most abundant actinomycetes in soil, and it has a massive contribution to drug industry 34,52 . The colour of aerial and substrate mycelium is tan in appearance if grown in ISP-2 and AIA medium.…”

Section: Resultsmentioning

confidence: 99%

Streptomyces sp SM01 isolated from Indian soil produces a novel antibiotic picolinamycin effective against multi drug resistant bacterial strains

Maiti

Sahoo

Mandal

2020

Sci Rep

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A Kashmir Himalayan (India) soil isolate, Streptomyces sp. SM01 was subjected to small scale fermentation for the production of novel antimicrobials, picolinamycin (SM1). The production has been optimized which found to be maximum while incubated in AIA medium (pH 7) for 7 days at 30 °C. Seven days grew crude cell-free culture media (50 µL) showed a larger zone of inhibition against Staphylococcus aureus compared to streptomycin (5 µg) and ampicillin (5 µg). Extraction, purification, and chemical analysis of the antimicrobial component has been proved to be a new class of antibiotic with 1013 dalton molecular weight. We have named this new antibiotic as picolinamycin for consisting picolinamide moiety in the center of the molecule and produced by a Streptomyces sp. In general, the antimicrobial potency of this newly characterized antibiotic found to be higher against Gram-positive organisms than the tested Gram-negative organisms. The MIC of this antimicrobial compound was found to be 0.01 µg/ml for tested Gram-positive organisms and 0.02 to 5.12 µg/ml for Gram-negative organisms. Furthermore, it showed strong growth impairments of several multidrug resistance (MDR) strains, including methicillin-resistant strains of Staphylococci and Enterococci with the MIC value of 0.04 to 5.12 µg/ml and MDR (but methicillin-sensitive) strains of S. aureus with the MIC value of 0.084 µg/ml. It also showed anti-mycobacterial potential in higher concentrations (MIC is 10.24 µg/ml). Picolinamycin however did not show toxicity against tested A549 human cell line indicating that the spectrum of its activity limited within bacteria only. About one out of ten patients are acquiring nosocomial infection as hospitals are the principal source of multidrug-resistant (MDR) pathogens 1. The most formidable MDR pathogens are methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus (VRE); vancomycin-resistant S. aureus (VRSA) and principal Gram-negative bacteria involved in nosocomial infection like Klebsiella pneumoniae, Acinetobacter baumanni, Pseudomonas aeruginosa, Enterobacter sp. which generally are also resistant against major existing antibiotics such as second-generation cephalosporin, fluoroquinolones, penicillin in combination with beta-lactamase inhibitor, third-generation cephalosporin and carbapenem 2-8. Non-judicial use of antibiotics is thought to be the major factor for antimicrobial resistance 8-10. More than 100,000 tons of antibiotics have been manufactured annually to combat pathogens 2. However most of the clinically used antibiotics against nosocomial bacteria remain ineffective due to their acquired resistance 11-14. From January 2000 to October 2019, only 44 compounds under 7 novel classes have been introduced in clinical pipeline 15. Among these 27 compounds are synthetic, 14 are derivative of natural product and 3 are from other sources. However the antimicrobials of natural origin are very rare, but these are found to be most suitable against multidrug resistance (MDR) bacteria, as the...

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Antimicrobial investigation of selected soil actinomycetes isolated from unexplored regions of Kashmir Himalayas, India

Cited by 39 publications

References 23 publications

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

The Draft Genome Sequence of The Endophytic Streptomyces Strain VITGV156

Streptomyces sp SM01 isolated from Indian soil produces a novel antibiotic picolinamycin effective against multi drug resistant bacterial strains

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