Antimicrobial nanocomposites and electrospun coatings based on poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) and copper oxide nanoparticles for active packaging and coating applications

Abstract

Active biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) melt mixed nanocomposites and bilayer structures containing cooper oxide (CuO) nanoparticles were developed and characterized. The bilayer structures consisted of a bottom layer of compression molded PHBV3 (3% mol valerate) coated with an active electrospun fibers mat made with microbial mixed culture derived PHBV18 (18 % valerate) and CuO nanoparticles. The results showed that the oxygen barrier properties were slightly enhanced by the addition of 0.05% CuO nanoparticles to nanocomposite films but a negligible effect was registered for the bilayer structures. Neither the water vapor permeability nor the mechanical properties were modified by the addition of CuO.Interestingly, by incorporating highly dispersed and distributed CuO nanoparticles in a coating by electrospinning, a lower metal oxide loading was required to exhibit significant bactericidal and virucidal performance against the food-borne pathogens Salmonella enterica, Listeria monocytogenes and Murine norovirus. The biodisintegration tests of the samples under composting conditions showed that even the 0.05% CuO coated structures biodegraded within 35 days.

Polymers and Biopolymers with Antiviral Activity: Potential Applications for Improving Food Safety

“…As reported for other antimicrobial materials, higher antibacterial activity against Salmonella enterica and Listeria monocytogenes was recorded under the same experimental conditions (Castro‐Mayorga et al., ).…”

“…Castro‐Mayorga, Fabra Rovira, Cabedo Mas, Sánchez Moragas, and Lagarón Cabello () recently developed and characterized 2 active copper‐based systems performing interesting antiviral activity. In their study, the antiviral activity of biodegradable PHBV melt mixed nanocomposites containing 0.1% and 0.05% of CuO was compared to bilayer structures consisting of a bottom layer of compression molded PHBV3 (3% mol valerate) coated with an active electrospun fibers layer made with microbial mixed culture‐derived PHBV18 (18% valerate) and CuO nanoparticles (0.05%).…”

Preparation and Characterization of Electrospun Food Biopackaging Films of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Derived From Fruit Pulp Biowaste

“…In comparison with other previous studies, the here-studied PHBV films exhibited a lower melting profile than other PHBV films prepared by solving casting, i.e., T m values at 145-157 • C for HV 12 mol.-% (Sanchez-Garcia et al, 2008). Similarly, PHBV materials with HVs of 3 and 18 mol.-% prepared by melt mixing also showed higher T m values, i.e., 169.6 and 173.2 • C, and T c values, i.e., 107.1 • C (Castro Mayorga et al, 2018). In relation to other electrospun films, (Cherpinski et al, 2018) reported two melting points at 162.2 and 177.3 • C for PHB films while T c was 110.4 • C. The lower values in the present study can be related to the higher HV content of the here-processed PHBV materials.…”

“…WAXD experiments were conducted on both films and fiber mats obtained from samples 6 and 7 to assess their crystallinity since DSC, being a dynamic method, does not provide an accurate description of crystallinity for this biopolymer as discussed earlier (Cherpinski et al, 2017;Castro Mayorga et al, 2018). The diffractograms, included in Figure 7, presented two main peaks located at 13.5 • and 16.9 • (2θ), followed by three other minor reflections at ca.…”

“…For example, electrospun mats made of PHBV derived from CW and containing zinc oxide (ZnO) were thermally post-processed as coatings on PHBV films to develop antimicrobial nanocomposites (Castro-Mayorga et al, 2017). Similarly, in another study, copper oxide (CuO) nanoparticles were loaded into PHBV electrospun coatings to form antimicrobial coatings with increased capacity (Castro Mayorga et al, 2018).…”