Antimicrobial peptide-based treatment for endodontic infections — Biotechnological innovation in endodontics

Lima, Stella Maris de Freitas; Pádua, Gabriela Martins de; Sousa, Maurício Gonçalves da Costa; Freire, Mirna de Souza; Franco, Octávio L.; Rezende, Taia Maria Berto

doi:10.1016/j.biotechadv.2014.10.013

Cited by 38 publications

(34 citation statements)

References 114 publications

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“…Transmembrane pore formation, intracellular targeting and metabolic inhibition mechanisms (inhibition of microbial functional proteins, DNA and RNA synthesis) [14,15] Synthetic AMPs (e.g. ␤-17, human neutrophil peptides 1 and 2, histatins 5 and 8) Cationic compounds Chitosan Interaction between positively charged chitosan molecules and negatively charged bacterial cell membranes leads to disruption of cell membrane [16] Chlorhexidine Bind to negatively charged bacterial walls and disrupt cell walls Poly(-lysine)…”

Section: Antibioticsmentioning

confidence: 99%

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Jiao

Niu

et al. 2017

Progress in Polymer Science

506

317

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a b s t r a c tMicrobial infections affect humans worldwide. Many quaternary ammonium compounds have been synthesized that are not only antibacterial, but also possess antifungal, antiviral and anti-matrix metalloproteinase capabilities. Incorporation of quaternary ammonium moieties into polymers represents one of the most promising strategies for preparation of antimicrobial biomaterials. Various polymerization techniques have been employed to prepare antimicrobial surfaces with quaternary ammonium functionalities; in particular, syntheses involving controlled radical polymerization techniques enable precise control over macromolecular structure, order and functionality. Although recent publications report exciting advances in the biomedical field, some of these technological developments have also been accompanied by potential toxicological and antimicrobial resistance challenges. Recent evidenced-based data on the biomedical applications of antimicrobial quaternary ammonium-containing biomaterials that are based on randomized human clinical trials, the golden standard in contemporary medicinal science, are included in the present review. This should help increase visibility, stimulate debates and spur conversations within a wider scientific community on the implications and plausibility for future developments of quaternary ammonium-based antimicrobial biomaterials.Published by Elsevier B.V.

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Section: Antibioticsmentioning

confidence: 99%

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Jiao

Niu

et al. 2017

Progress in Polymer Science

506

317

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“…Antimicrobial peptides (AMPs) have a wide spectrum of antimicrobial activity against oral microorganisms, and there was a study about the antimicrobial peptide-based treatment for endodontic infections using several AMPs that are endogenous to the oral cavity. 15 These include human β-defensins (HBDs), which are cationic peptides expressed in inflamed dental pulps. 16 HBDs (HBD1, 2, 3, and 4) have antimicrobial properties, and HBD3 was found to be the most potent.…”

Section: Introductionmentioning

confidence: 99%

Antifungal effects of synthetic human β-defensin 3-C15 peptide

et al. 2016

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ObjectivesThe purpose of this ex vivo study was to compare the antifungal activity of a synthetic peptide consisting of 15 amino acids at the C-terminus of human β-defensin 3 (HBD3-C15) with calcium hydroxide (CH) and Nystatin (Nys) against Candida albicans (C. albicans) biofilm.Materials and MethodsC. albicans were grown on cover glass bottom dishes or human dentin disks for 48 hr, and then treated with HBD3-C15 (0, 12.5, 25, 50, 100, 150, 200, and 300 µg/mL), CH (100 µg/mL), and Nys (20 µg/mL) for 7 days at 37℃. On cover glass, live and dead cells in the biomass were measured by the FilmTracer Biofilm viability assay, and observed by confocal laser scanning microscopy (CLSM). On dentin, normal, diminished and ruptured cells were observed by field-emission scanning electron microscopy (FE-SEM). The results were subjected to a two-tailed t-test, a one way analysis variance and a post hoc test at a significance level of p = 0.05.ResultsC. albicans survival on dentin was inhibited by HBD3-C15 in a dose-dependent manner. There were fewer aggregations of C. albicans in the groups of Nys and HBD3-C15 (≥ 100 µg/mL). CLSM showed C. albicans survival was reduced by HBD3-C15 in a dose dependent manner. Nys and HBD3-C15 (≥ 100 µg/mL) showed significant fungicidal activity compared to CH group (p < 0.05).ConclusionsSynthetic HBD3-C15 peptide (≥ 100 µg/mL) and Nys exhibited significantly higher antifungal activity than CH against C. albicans by inhibiting cell survival and biofilm.

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“…The use of antimicrobial agents able to eliminate resistant species from root canals and to modulate the periapical inflammatory-immune response may improve the success rate of endodontic treatments (Turner et al 2004;Lima et al 2015). In the field of endodontics, AMPs have been studied as irrigants, used during chemomechanical preparation and as intracanal medicament, applied between treatment sessions.…”

Section: Discussionmentioning

confidence: 99%

A New Combination with D-Cateslytin to Eradicate Root Canal Pathogens

Ehlinger

Dartevelle

Zaet

et al. 2019

Int J Pept Res Ther

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The success of endodontic treatments depends on the elimination of intracanal pathogens. Since irrigation and instrumentation can only partially eliminate bacteria, the use of intracanal medicaments is suggested to improve the eradication of the root canal pathogens. Antimicrobial peptides derived from Chromogranin A display bacteriolytic activities and are potentially excellent candidates to be combined with conventional intracanal medicaments. In this study, we combined D-Cateslytin (D-Ctl), together with calcium hydroxide (Ca(OH) 2) to test for enhanced antimicrobial properties against Enterococcus faecalis. Antimicrobial activities were determined by broth dilution assays, stability of D-Ctl was assessed by HPLC and MTT tests were used to evaluate cytotoxicity. A saturated solution of Ca(OH) 2 (1.7 mg/mL) was able to inhibit 58% (± 5%) of E. faecalis growth, while the combination of both 0.85 mg/mL of Ca(OH) 2 and ½ MIC of D-Ctl was able to fully inhibit its growth. D-Ctl was stable against the proteases secreted by E. faecalis and showed no toxicity on human gingival fibroblasts. Besides E. faecalis, this combination was also effective in completely killing other endodontic pathogens: Parvimonas micra, Prevotella intermedia, Fusobacterium nucleatum and Candida albicans. In conclusion, D-Ctl combined with Ca(OH) 2 eradicates several endodontic pathogens and could be used as an innovative intracanal medicament to reduce endodontic failures.

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Antimicrobial peptide-based treatment for endodontic infections — Biotechnological innovation in endodontics

Cited by 38 publications

References 114 publications

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Antifungal effects of synthetic human β-defensin 3-C15 peptide

A New Combination with D-Cateslytin to Eradicate Root Canal Pathogens

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