Antimicrobial-peptide coating that ruptures the wall of Gram positive bacteria

Chen, X.; Hirt, Helmut; Li, Y.; Gorr, Sven Ulrik; Aparicio, Conrado

doi:10.1016/j.dental.2014.08.177

Cited by 2 publications

(2 citation statements)

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“…177 Previous research has demonstrated that the antimicrobial peptide GL13K, derived from the human salivary protein (BPIFA2), can be covalently bonded to titanium surfaces. 178 This covalently immobilized GL13K coating has exhibited excellent antimicrobial activity against P. gingivalis and other oral pathogens associated with biofilm formation. 179 Clinical studies evaluating synthetic AMPs are critical for determining their efficacy and safety in treating infections caused by pathogenic bacteria.…”

Section: ■ Antibioticsmentioning

confidence: 99%

Addressing Antimicrobial Properties in Guided Tissue/Bone Regeneration Membrane: Enhancing Effectiveness in Periodontitis Treatment

Takallu,

Mirzaei,

Zakeri Bazmandeh

et al. 2024

ACS Infect. Dis.

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Guided tissue regeneration (GTR) and guided bone regeneration (GBR) are the two surgical techniques generally used for periodontitis disease treatment. These techniques are based on a barrier membrane to direct the growth of new bone and gingival tissue at sites with insufficient volumes or dimensions of bone or gingiva for proper function, esthetics, or prosthetic restoration. Numerous studies have highlighted biocompatibility, space-creation, cell-blocking, bioactivity, and proper handling as essential characteristics of a membrane's performance. Given that bacterial infection is the primary cause of periodontitis, we strongly believe that addressing the antimicrobial properties of these membranes is of utmost importance. Indeed, the absence of effective inhibition of periodontal pathogens has been recognized as a primary factor contributing to the failure of GTR/GBR membranes. Therefore, we suggest considering antimicrobial properties as one of the key factors in the design of GTR/GBR membranes. Antibiotics are potent medications frequently administered systemically to combat microbes and mitigate bacterial infections. Nevertheless, the excessive use of antibiotics has resulted in a surge in bacterial resistance. To overcome this challenge, alternative antibacterial substances have been developed. In this review, we explore the utilization of alternative substances with antimicrobial properties for topical application in membranes. The use of antibacterial nanoparticles, phytochemical compounds, and antimicrobial peptides in this context was investigated. By carefully selecting and integrating antimicrobial agents into GTR/GBR membranes, we can significantly enhance their effectiveness in combating periodontitis. These antibacterial substances not only act as barriers against pathogenic bacteria but also promote the process of periodontal healing.

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Section: ■ Antibioticsmentioning

confidence: 99%

Addressing Antimicrobial Properties in Guided Tissue/Bone Regeneration Membrane: Enhancing Effectiveness in Periodontitis Treatment

Takallu,

Mirzaei,

Zakeri Bazmandeh

et al. 2024

ACS Infect. Dis.

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“…Chou−Fasman was used as an initial estimation tool in developing rules for the antimicrobial peptide film property. We identified two AMPs with low MIC: AMPA, 80 comprised of 60% predicted α-helix forming amino acids and GL13K 81 containing no predicted α-helix forming amino acids (Table 1). The MIC for each AMP is given is Table S1.…”

Section: Acs Biomaterials Science and Engineeringmentioning

confidence: 99%

Mitigation of Peri-implantitis by Rational Design of Bifunctional Peptides with Antimicrobial Properties

Wisdom

Zhou

Chen

et al. 2019

ACS Biomater. Sci. Eng.

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The integration of molecular and cell biology with materials science has led to strategies to improve the interface between dental implants with the surrounding soft and hard tissues in order to replace missing teeth and restore mastication. More than 3 million implants have been placed in the US alone and this number is rising by 500,000/year. Peri-implantitis, an inflammatory response to oral pathogens growing on the implant surface threatens to reduce service life leading to eventual implant failure, and such an outcome will have adverse impact on public health and create significant health care costs. Here we report a predictive approach to peptide design, which enabled us to engineer a bifunctional peptide to combat bacterial colonization and biofilm formation, reducing the adverse host inflammatory immune response that destroys the tissue surrounding implants and shortens their lifespans. This bifunctional peptide contains a titaniumbinding domain that recognizes and binds with high affinity to titanium implant surfaces, fused through a rigid spacer domain with an antimicrobial domain. By varying the antimicrobial peptide domain, we were able to predict the properties of the resulting bifunctional peptides in their entirety by analyzing the sequence-structure-function relationship. These bifunctional peptides achieve: 1) nearly 100% surface coverage within minutes, a timeframe suitable for their clinical application to existing implants; 2) nearly 100% binding to a titanium surface even in the presence of contaminating serum protein; 3) durability to brushing with a commercially available electric toothbrush; and 4) retention of antimicrobial activity on the implant surface following bacterial challenge. A bifunctional peptide film can be applied to both new implants and/or repeatedly applied to previously placed implants to control bacterial colonization mitigating peri-implant disease that threatens dental implant longevity.

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Antimicrobial-peptide coating that ruptures the wall of Gram positive bacteria

Cited by 2 publications

References 0 publications

Addressing Antimicrobial Properties in Guided Tissue/Bone Regeneration Membrane: Enhancing Effectiveness in Periodontitis Treatment

Addressing Antimicrobial Properties in Guided Tissue/Bone Regeneration Membrane: Enhancing Effectiveness in Periodontitis Treatment

Mitigation of Peri-implantitis by Rational Design of Bifunctional Peptides with Antimicrobial Properties

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