Antimicrobial peptide functionalized gold nanorods combining near-infrared photothermal therapy for effective wound healing

Xu, Xinyu; Ding, Yujie; Hadianamrei, Roja; Lv, Songwei; You, Rongrong; Pan, Fang; Zhang, Peng; Wang, Nan; Zhao, Xiaoyong

doi:10.1016/j.colsurfb.2022.112887

Cited by 20 publications

(16 citation statements)

References 48 publications

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“…Pt5 AMP, which is produced from zebrafish phosvitin, is the source of Pt5-1c, an AMP that aids in wound healing [ 56 ]. A synthetic AMP called C-At5 is created by adding a cysteine residue to the N terminus of At5 [ 57 ]. The synthetic AMP Esc(1-21) has 21 residues and is generated from Esculentin-1A, the parent AMP.…”

Section: Reviewmentioning

confidence: 99%

“…According to Xu et al (2022), C-At5 is biocompatible and has less toxicity when combined with AuNRs. AuNR@C-At5 exhibited a hemolysis rate of less than 5% in human red blood cells, suggesting a high degree of compatibility with blood cells [ 57 ]. Conversely, at 80 μg/mL, AuNR@CTAB itself had a 60% hemolysis rate, making it extremely hazardous [ 57 ].…”

Section: Reviewmentioning

confidence: 99%

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A Systematic Review of the Design and Applications of Antimicrobial Peptides in Wound Healing

Ahmad,

Khan,

Bandy

2024

Cureus

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The sources of antimicrobial peptides (AMPs), also known as peptide-based antibiotics, are diverse, such as plants, animals, microorganisms including human leukocytes, saliva, human defense peptides, and human sweat. These natural sources provide a rich variety of AMPs with unique characteristics and potential therapeutic applications, including wound-healing and antimicrobial properties. AMPs derived from these sources have shown promise in combating a wide range of pathogens, making them valuable targets for further research and potential clinical applications. The design of AMPs for wound healing involves a meticulous process of structurally optimizing peptides to possess a unique combination of antibacterial and wound-healing characteristics. This systematic review was produced to show the design and applications of AMPs in wound healing. The terms "antimicrobial peptides AND wound healing" were used to search for articles published between September 2023 and January 2010. In the search, we found a total of 12958 articles, of which 12898 were excluded, and the remaining 60 articles were chosen for further study. This systematic review underscores the potential of AMPs as valuable tools in infection control and wound healing, showcasing their versatility and effectiveness in combating a wide range of pathogens. Overall, AMPs in wound healing display a diverse mechanism of action, influencing the inflammatory response, encouraging tissue regeneration, and aiding tissue remodeling, along with strong antibacterial activity. Furthermore, this systematic review addresses AMP toxicity studies, which include rigorous in vitro and in vivo examinations to determine potential cytotoxic effects, systemic toxicity, and any adverse responses connected with its usage in wound-healing applications.

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Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

A Systematic Review of the Design and Applications of Antimicrobial Peptides in Wound Healing

Ahmad,

Khan,

Bandy

2024

Cureus

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“…Mass spectrometry and amino acid analysis can provide an average estimate of the number of peptides per AuNP . Quantifying unbound ligands remaining in the supernatant offers a mass balance approach to obtaining a similar result. ,, In combination, these analytical techniques can provide evidence of successful peptide conjugation to the AuNP surface. Nevertheless, robust data showing the density and packing of peptides on the AuNP surface are lacking and perhaps can be addressed with nuclear magnetic resonance (NMR) spectroscopy and scanning-transmission electron microscopy–electron energy loss spectroscopy (STEM-EELS) characterization as discussed below.…”

Section: Categories Of Soft Surface Coatings and Ligandsmentioning

confidence: 99%

The Landscape of Gold Nanocrystal Surface Chemistry

et al. 2023

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Conspectus Gold nanoparticles (AuNPs) exhibit unique size- and shape-dependent properties not obtainable at the macroscale. Gold nanorods (AuNRs), with their morphology-dependent optical properties, ability to convert light to heat, and high surface-to-volume ratios, are of great interest for biosensing, medicine, and catalysis. While the gold core provides many fascinating properties, this Account focuses on AuNP soft surface coatings, which govern the interactions of nanoparticles with the local environments. Postmodification of AuNP surface chemistry can greatly alter NP colloidal stability, nano-bio interactions, and functionality. Polyelectrolyte coatings provide controllable surface-coating thickness and charge, which impact the composition of the acquired corona in biological settings. Covalent modification, in which covalently bound ligands replace the original capping layer, is often performed with thiols and disulfides due to their ability to replace native coatings. N-heterocyclic carbenes and looped peptides expand the possible functionalities of the ligand layer. The characterization of surface ligands bound to AuNPs, in terms of ligand density and dynamics, remains a challenge. Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for understanding molecular structures and dynamics. Our recent NMR work on AuNPs demonstrated that NMR data were obtainable for ligands on NPs with diameters up to 25 nm for the first time. This was facilitated by the strong proton NMR signals of the trimethylammonium headgroup, which are present in a distinct regime from other ligand protons’ signals. Ligand density analyses showed that the smallest AuNPs (below 4 nm) had the largest ligand densities, yet spin–spin T2 measurements revealed that these smallest NPs also had the most mobile ligand headgroups. Molecular dynamics simulations were able to reconcile these seemingly contradictory results. While NMR spectroscopy provides ligand information averaged over many NPs, the ligand distribution on individual particles’ surfaces must also be probed to fully understand the surface coating. Taking advantage of improvements in electron energy loss spectroscopy (EELS) detectors employed with scanning transmission electron microscopy (STEM), a single-layer graphene substrate was used to calibrate the carbon K-edge EELS signal, allowing quantitative imaging of the carbon atom densities on AuNRs with sub-nanometer spatial resolution. In collaboration with others, we revealed that the mean value for surfactant-bilayer-coated AuNRs had 10–30% reduced ligand density at the ends of the rods compared to the sides, confirming prior indirect evidence for spatially distinct ligand densities. Recent work has found that surface ligands on nanoparticles can, somewhat surprisingly, enhance the selectivity and efficiency of the electrocatalytic reduction of CO2 by controlling access to the active site, tuning its electronic and chemical environment, or denying entry to impurities that poison the nanoparticle surface to facilitate ...

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“…Antimicrobial peptides (AMPs) or host defense peptides are naturally occurring peptides present in different organisms which are capable of killing pathogens through non-receptor-mediated membrane damage. [16][17][18][19][20][21][22][23][24] Physical damage to microbial membranes greatly reduces the chance of AMR. 19,[21][22][23][24][25][26] This advantage has drawn great attention to AMPs as novel antibiotics.…”

Section: Introductionmentioning

confidence: 99%

“…[16][17][18][19][20][21][22][23][24] Physical damage to microbial membranes greatly reduces the chance of AMR. 19,[21][22][23][24][25][26] This advantage has drawn great attention to AMPs as novel antibiotics.…”

Section: Introductionmentioning

confidence: 99%