Antimicrobial peptide S100A12 (calgranulin C) inhibits growth, biofilm formation, pyoverdine secretion and suppresses type VI secretion system in Pseudomonas aeruginosa

Mishra, Priyasha; Ch, Sanjay; Hong, Seok Jong; Biswas, Swati; Roy, Sanhita

doi:10.1016/j.micpath.2022.105654

Cited by 14 publications

(9 citation statements)

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“…Expression of genes related to the structural component of type 6 secretion system (T6SS); pldA, icmF1, hcp1, and vrgG1, are also diminished. This figure is based on Mishra P et al (2022) [98] publication its effectiveness. On the other hand, the addition of the low molecular weight alginate oligosaccharide OligoG CF-5/20, derived from the stem of brown algae Laminaria hyperborean, enhanced colistin diffusion in mucin-rich AS medium, enhanced mucus penetration by colistin, and significantly intensified colistin antimicrobial activity against mucoid P. aeruginosa biofilms [109].…”

Section: Additional Methods For Restricting Pseudomonas Aeruginosa In...mentioning

confidence: 99%

“…It is worth noting that human body naturally produces antimicrobial peptides that can combat infections driven by bacterial pathogens. For example, the antimicrobial peptides S100A12 (calgranulin C) is significantly expressed in immune cells like neutrophils and macrophages in addition to corneal tissues of patients with Pseudomonas keratitis [ 97 , 98 ]. Its roles on P. aeruginosa growth, biofilm formation, pyoverdine secretion and type VI secretion system have been depicted in Fig.…”

Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

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Recent advances in therapeutic targets identification and development of treatment strategies towards Pseudomonas aeruginosa infections

et al. 2023

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The opportunistic human pathogen Pseudomonas aeruginosa is the causal agent of a wide variety of infections. This non-fermentative Gram-negative bacillus can colonize zones where the skin barrier is weakened, such as wounds or burns. It also causes infections of the urinary tract, respiratory system or bloodstream. P. aeruginosa infections are common in hospitalized patients for which multidrug-resistant, respectively extensively drug-resistant isolates can be a strong contributor to a high rate of in-hospital mortality. Moreover, chronic respiratory system infections of cystic fibrosis patients are especially concerning, since very tedious to treat. P. aeruginosa exploits diverse cell-associated and secreted virulence factors, which play essential roles in its pathogenesis. Those factors encompass carbohydrate-binding proteins, quorum sensing that monitor the production of extracellular products, genes conferring extensive drug resistance, and a secretion system to deliver effectors to kill competitors or subvert host essential functions. In this article, we highlight recent advances in the understanding of P. aeruginosa pathogenicity and virulence as well as efforts for the identification of new drug targets and the development of new therapeutic strategies against P. aeruginosa infections. These recent advances provide innovative and promising strategies to circumvent infection caused by this important human pathogen.

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Section: Additional Methods For Restricting Pseudomonas Aeruginosa In...mentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

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Recent advances in therapeutic targets identification and development of treatment strategies towards Pseudomonas aeruginosa infections

et al. 2023

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“…In this study, S100A12 was found to have downregulated the expression of biofilm formation genes, redox pathways, and pyoverdine synthesis of the bacteria. [32] Here, we have developed 3D-printed contact lenses-like patches using hydrogels loaded with AMP (S100A12) for treating the BK-infected rabbit model.…”

Section: Introductionmentioning

confidence: 99%

3D‐Printed Inherently Antibacterial Contact Lens‐Like Patches Carrying Antimicrobial Peptide Payload for Treating Bacterial Keratitis

Ch,

Mishra,

Padaga

et al. 2024

Macromolecular Bioscience

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Delivery of therapeutic agents through contact lenses‐like patches is a promising strategy to achieve significant bioavailability with negligible eye drainage. The present study investigates the preparation and three‐dimensional (3D) printing of mucoadhesive Gelatin Methacryloyl (GelMA)/ Chitosan Methacryloyl (ChiMA) hydrogels to fabricate them as contact lens‐like patches (CLP) loaded with antimicrobial peptide, S100A12 (AMP) for treating bacterial keratitis (BK). Extrusion technology was used to print the patches layer by layer to form a hemispherical scaffold suitable for eyewear, and 3D printed CLP were crosslinked using Irgacure 2959 under UV light. The results from the in vivo experiment conducted on P. aeruginosa‐infected BK rabbit model after the treatment with AMP‐loaded CLP have shown a significant decrease in bacterial load when plated for CFU. The newly developed delivery system containing AMP has great potential to overcome the treatment challenges of multidrug resistance (MDR) in bacteria and eliminate the frequent dosing associated with eye drops. The presence of chitosan in the formulation provided a synergetic effect on the AMP in disrupting bacterial biofilms. The ease of using 3D printing would open new avenues for optimizing the dosage depending on the severity of the BK in the patients, which can be used as personalized medicine.This article is protected by copyright. All rights reserved

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“…Interestingly, studies correlate the pathogenicity of Pseudomonas to the T6SS which mediates biofilm formation (Li et al, 2020 ). Therefore, it becomes a target for antimicrobial agents (Mishra et al, 2022 ). In Bordetella spp., T3SS is mainly expressed and involved in colonization as well as the hijacking of the host immune responses.…”

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confidence: 99%

Editorial: Microbial sensing to control host immune responses

Sayed

Bhattacharyya²,

Das³

2022

Front. Microbiol.

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Antimicrobial peptide S100A12 (calgranulin C) inhibits growth, biofilm formation, pyoverdine secretion and suppresses type VI secretion system in Pseudomonas aeruginosa

Cited by 14 publications

References 67 publications

Recent advances in therapeutic targets identification and development of treatment strategies towards Pseudomonas aeruginosa infections

Recent advances in therapeutic targets identification and development of treatment strategies towards Pseudomonas aeruginosa infections

3D‐Printed Inherently Antibacterial Contact Lens‐Like Patches Carrying Antimicrobial Peptide Payload for Treating Bacterial Keratitis

Editorial: Microbial sensing to control host immune responses

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