Antimicrobial peptide selection from Lippia spp leaf transcriptomes

Tavares, Letícia Stephan; Souza, Vinícius Carius de; Nunes, Vinicius Schmitz; Souza, Gustavo Torres de; Marques, Lucas Farinazzo; Goliatt, Priscila Vanessa Zabala Capriles; Viccini, Lyderson Facio; Franco, Octávio L.; Santos, Marcelo de Oliveira

doi:10.1016/j.peptides.2020.170317

Cited by 11 publications

(6 citation statements)

References 49 publications

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“…The large scale of cDNA library members, which can be thousands, makes the screening for AMPs quite challenging. However, in recent years, many studies have used the cDNA library method to clone AMPs (Ronèeviae et al, 2018;Wu et al, 2019;Tavares et al, 2020) due to its advantages such as enhanced fragments of efficiently transcribed genes and cloned AMPs from cDNA library, which can be actively manufactured via bacterial expression systems (Sharma et al, 2014) due to the lack of introns that might pose a problem when the target is manufactured as a eukaryotic protein in bacteria. In our lab, we screened cDNA libraries for AMPs via expression systems (Kong et al, 2018;Wu et al, 2020), but this is also a time-and cost-consuming approach.…”

Section: Introductionmentioning

confidence: 99%

Prediction and Characterization of Cationic Arginine-Rich Plant Antimicrobial Peptide SM-985 From Teosinte (Zea mays ssp. mexicana)

2020

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Antimicrobial peptides (AMPs) are effective against different plant pathogens and newly considered as part of plant defense systems. From prokaryotes to eukaryotes, AMPs can exist in all forms of life. SM-985 is a cationic AMP (CAMP) isolated from the cDNA library of Mexican teosinte (Zea mays ssp. mexicana). A computational prediction server running with different algorithms was used to screen the teosinte cDNA library for AMPs, and the SM-985 peptide was predicted as an AMP with high probability prediction values. SM-985 is an arginine-rich peptide and composed of 21 amino acids (MW: 2671.06 Da). The physicochemical properties of SM-985 are very promising as an AMP, including the net charge (+8), hydrophobicity ratio of 23%, Boman index of 5.19 kcal/mol, and isoelectric point of 12.95. The SM-985 peptide has amphipathic α-helix conformations. The antimicrobial activity of SM-985 was confirmed against six bacterial plant pathogens, and the MIC of SM-985 against Gram-positive indicators was 8 µM, while the MIC of SM-985 against Gram-negative indicators was 4 µM. The SM-985 interacting with the bacterial membrane and this interaction were examined by treatment of the bacterial indicators with FITC-SM-985 peptide, which showed a high binding affinity of SM-985 to the bacterial membrane (whether Gram-positive or Gram-negative). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images of the treated bacteria with SM-985 demonstrated cell membrane damage and cell lysis. In vivo antimicrobial activity was examined, and SM-985 prevented leaf spot disease infection caused by Pst DC3000 on Solanum lycopersicum. Moreover, SM-985 showed sensitivity to calcium chloride salt, which is a common feature of CAMPs.

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Section: Introductionmentioning

confidence: 99%

Prediction and Characterization of Cationic Arginine-Rich Plant Antimicrobial Peptide SM-985 From Teosinte (Zea mays ssp. mexicana)

2020

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“…Dentre essas plantas medicinais comumente usadas, destaca-se a Lippia Linn, algumas espécies desse gênero se destacam por possuírem possuir aparência chamativa no período de floração e aroma forte, característico do óleo essencial e por seus potenciais farmacológicos (Vilela et al, 2020). Além disso, as plantas deste gênero compreendem um grande número de espécies medicinais, que são amplamente utilizadas na medicina tradicional brasileira para tratar doenças como gastrite, diarreia, febre, asma, além de promover sedação, por apresentarem atividades biológicas (Tavares et al, 2020). Diante disso, foi realizado um estudo bibliográfico sistematizado na base de dados Scielo sobre o uso etnobotânico de Lippia Linn e demonstrar se houveram intoxicações com a utilização de algumas plantas desse gênero (Tabela 1).…”

Section: Resultsunclassified

Uso etnobotânico da Lippia Linn (Verbenaceae): uma revisão de literatura

Soares

Sousa²,

Passos³

2022

RSD

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O Brasil possui a maior biodiversidade genética do mundo, o que estimula do uso e desenvolvimento de plantas medicinais. As plantas medicinais possuem princípios bioativos e propriedades farmacológicas, promovendo assim uma interação entre plantas e pessoas. Dentre essas plantas, destaca-se a Lippia Lin.n, popularmente conhecida como melissa ou falsa-melissa, erva-cidreira, erva-cidreira-de-arbusto ou cidreira-brasileira, chá-de-tabuleiro, salva-limão, alecrim-do-campo, muito utilizada pelas pessoas no tratamento e prevenção de doenças. Contudo, com o uso inapropriado dessas plantas, as intoxicações podem ocorrer em razão da ingestão de quantidades excessivas, ou do preparo inadequado. Portanto, o objetivo dessa pesquisa foi identificar a prevalência do uso da Lippia Linn. na etnobotânica através da base de dados Scielo e avaliar se já houveram intoxicações com o uso da mesma. Diante disso, foi realizado um levantamento bibliográfico sistematizado na base de dados Scielo sobre o uso etnobotânico de Lippia Linn. Os descritores utilizados nas pesquisas foram “etnobotânico”, “Lippia”, “intoxicação” junto ao operador booleano or, selecionando os trabalhos publicados entre os anos de 2018 a 2020. Foram utilizados os critérios de inclusão estudos que relacionavam o uso etnobotânico da Lippia Linn., intoxicações que podem ter sido causadas pelo uso da planta e artigos no idioma português e inglês, publicados nos últimos dois anos.

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“…As a result, this approach identifies inadequacies, discusses the most probable origin, and suggests ways to feasibly resolve them. However, several cDNA library techniques have been studied to clone AMPs in recent years [92][93][94] because of their positive effects, such as correctly transcribed genes and cloning ability [95]. Moreover, the absence of introns allows engineering via a bacterial expression system.…”

Section: Cdna Library Based Plant Amps Isolationmentioning

confidence: 99%

Antimicrobial Peptides from Plants: A cDNA-Library Based Isolation, Purification, Characterization Approach and Elucidating Their Modes of Action

Islam

Mohamed

Polash

et al. 2021

IJMS

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Even in a natural ecosystem, plants are continuously threatened by various microbial diseases. To save themselves from these diverse infections, plants build a robust, multilayered immune system through their natural chemical compounds. Among the several crucial bioactive compounds possessed by plants' immune systems, antimicrobial peptides (AMPs) rank in the first tier. These AMPs are environmentally friendly, anti-pathogenic, and do not bring harm to humans. Antimicrobial peptides can be isolated in several ways, but recombinant protein production has become increasingly popular in recent years, with the Escherichia coli expression system being the most widely used. However, the efficacy of this expression system is compromised due to the difficulty of removing endotoxin from its system. Therefore, this review suggests a high-throughput cDNA library-based plant-derived AMP isolation technique using the Bacillus subtilis expression system. This method can be performed for large-scale screening of plant sources to classify unique or homologous AMPs for the agronomic and applied field of plant studies. Furthermore, this review also focuses on the efficacy of plant AMPs, which are dependent on their numerous modes of action and exceptional structural stability to function against a wide range of invaders. To conclude, the findings from this study will be useful in investigating how novel AMPs are distributed among plants and provide detailed guidelines for an effective screening strategy of AMPs.

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Antimicrobial peptide selection from Lippia spp leaf transcriptomes

Cited by 11 publications

References 49 publications

Prediction and Characterization of Cationic Arginine-Rich Plant Antimicrobial Peptide SM-985 From Teosinte (Zea mays ssp. mexicana)

Prediction and Characterization of Cationic Arginine-Rich Plant Antimicrobial Peptide SM-985 From Teosinte (Zea mays ssp. mexicana)

Uso etnobotânico da Lippia Linn (Verbenaceae): uma revisão de literatura

Antimicrobial Peptides from Plants: A cDNA-Library Based Isolation, Purification, Characterization Approach and Elucidating Their Modes of Action

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