Antimicrobial Peptide-Tether Dressing Able to Enhance Wound Healing by Tissue Contact

Abstract

No effective therapeutic dressings are currently available in the market that can prevent bacterial infection and simultaneously promote skin regeneration in diabetic patients. The lack of re-epithelization, prevalence of inflammation, and high risk of infection are hallmarks of non-healing wounds. Here, we have evaluated the antimicrobial and pro-regenerative effect of a relatively non-leaching LL37 peptide immobilized in polyurethane (PU)-based wound dressings (PU-adhesive-LL37 dressing). The PU-adhesive-LL37 (63 μg LL37NPs/cm 2 ) dressing killed Grampositive and Gram-negative bacteria in human serum without inducing bacterial resistance after 16 antimicrobial test cycles in contrast to commercially available dressings with the capacity to release antimicrobial Ag ions. Importantly, type II diabetic mice (db/db mice) treated with the PU-adhesive-LL37 dressing for different periods of time (6 or 14 days) showed enhanced wound healing and re-epithelialization (i.e., high keratin 14/5 levels) and lower macrophage infiltration in the wounds compared to animals treated with PU. The wounds treated with PU-adhesive-LL37 dressings showed also low expression of pro-inflammatory cytokines such as TNF-α and IL6 after 6 days of treatment, indicating that they act as an anti-inflammatory dressing. Additionally, PUadhesive-LL37 dressings do not induce acute inflammatory responses in the peripheral blood mononuclear cells (PBMCs) after 3 days of exposure, in contrast to controls. Taken together, PU-adhesive-LL37NP dressings might prevent the bacterial infections and facilitate wound healing by tissue contact, inducing re-epithelialization and anti-inflammatory processes in diabetic conditions.

Janus Intelligent Antibacterial Hydrogel Dressings for Chronic Wound Healing in Diabetes

“…After 11 days of treatment, the wound area in the PBS, DuoDerm dressing, SHC hydrogel, and SHGC hydrogel groups decreased to 30.21 ± 2.68, 12.14 ± 3.11, 13.91 ± 3.26, and 4.19 ± 1.89%. Interestingly, SHGC hydrogel-treated wounds achieved almost complete closure on day 11, exhibiting a faster healing rate than previously reported dressings . This may be attributed to the antimicrobial properties provided by the sustained release of GS and the moist wound environment provided by the hydrogel.…”

“…Interestingly, SHGC hydrogel-treated wounds achieved almost complete closure on day 11, exhibiting a faster healing rate than previously reported dressings. 31 This may be attributed to the antimicrobial properties provided by the sustained release of GS and the moist wound environment provided by the hydrogel. Apparently, the SHGC group had a significant difference from other groups in terms of the wound healing speed (Figure 6E), indicating that the SHGC hydrogel has excellent biocompatibility and can promote the repair and regeneration of chronic wound skin.…”

Alginate-Based Cryogels for Combined Chemo/Photothermal Antibacterial Therapy and Rapid Hemostasis

“…Moreover, owing to its good biocompatibility, biodegradability, and hemostatic ability, SA has great application potential in inventing wound dressing platforms in clinical use. − However, SA shows no antibacterial property, and most of the patients lost their protection of skin at the wound part, which can easily cause bacterial infections, leading to serious consequences. To overcome the problem, combining with additional active ingredients (e.g., antibiotics) endows the wound dressing effective antibacterial property, but enhanced/abusive use of antibiotics causes very serious side effects and leads to drug resistance, even the creation of superbug. − Thus, it is imperative to develop more efficient SA-based wound dressing materials with antibacterial capability in a non-antibiotic way.…”

Nanosized Shikonin-Fe(III) Coordination Material for Synergistic Wound Treatment: An Initial Explorative Study

“…Therefore, locally administered drugs should be preferably effective in both anti-inflammatory and antimicrobial activities with low drug resistance. In this study, we synthesized Shik-Fe nanoparticles by a one-pot procedure, and it was used in scald wounds with bacterial infection. The synergy of anti-inflammation and anti-bacterial activity was simultaneously realized within the Shik-Fe structure to promote the healing of infected scald.…”