Antimicrobial peptides interact with peptidoglycan

Neelay, Om P.; Peterson, Christian A.; Snavely, Mary E.; Brown, Taylor C.; TecleMariam, Ariam F.; Campbell, Jon; Blake, Allison; Schneider, Sydney C.; Cremeens, Matthew E.

doi:10.1016/j.molstruc.2017.06.018

Cited by 24 publications

(20 citation statements)

References 66 publications

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“…faecium BM4147 leads to vancomycin resistance. Consistent with a recent report [ 50 ] showing that melittin and cecropin A interact with peptidoglycan, our data strongly support a role for peptidoglycan as a potentiator of DMS-DA6-NH 2 activity, likely by trapping the peptide close to the bacterial membrane, increasing its local concentration, thus amplifying its action. Since the C-terminal part of both DMS-DA6 peptides is highly flexible, the carboxylate moiety could act as a repulsive group and curb binding of DMS-DA6-OH to PGN, a situation not occurring for DMS-DA6-NH 2 .…”

Section: Discussionsupporting

confidence: 92%

Peptidoglycan potentiates the membrane disrupting effect of the carboxyamidated form of DMS-DA6, a Gram-positive selective antimicrobial peptide isolated from Pachymedusa dacnicolor skin

Cardon¹,

Sachon²,

Carlier³

et al. 2018

PLoS ONE

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The occurrence of nosocomial infections has been on the rise for the past twenty years. Notably, infections caused by the Gram-positive bacteria Staphylococcus aureus represent a major clinical problem, as an increase in antibiotic multi-resistant strains has accompanied this rise. There is thus a crucial need to find and characterize new antibiotics against Gram-positive bacteria, and against antibiotic-resistant strains in general. We identified a new dermaseptin, DMS-DA6, produced by the skin of the Mexican frog Pachymedusa dacnicolor, with specific antibacterial activity against Gram-positive bacteria. This peptide is particularly effective against two multiple drug-resistant strains Enterococcus faecium BM4147 and Staphylococcus aureus DAR5829, and has no hemolytic activity. DMS-DA6 is naturally produced with the C-terminal carboxyl group in either the free or amide forms. By using Gram-positive model membranes and different experimental approaches, we showed that both forms of the peptide adopt an α-helical fold and have the same ability to insert into, and to disorganize a membrane composed of anionic lipids. However, the bactericidal capacity of DMS-DA6-NH2 was consistently more potent than that of DMS-DA6-OH. Remarkably, rather than resulting from the interaction with the negatively charged lipids of the membrane, or from a more stable conformation towards proteolysis, the increased capacity to permeabilize the membrane of Gram-positive bacteria of the carboxyamidated form of DMS-DA6 was found to result from its enhanced ability to interact with peptidoglycan.

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Section: Discussionsupporting

confidence: 92%

Peptidoglycan potentiates the membrane disrupting effect of the carboxyamidated form of DMS-DA6, a Gram-positive selective antimicrobial peptide isolated from Pachymedusa dacnicolor skin

Cardon¹,

Sachon²,

Carlier³

et al. 2018

PLoS ONE

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“…Flavonoid mengakibatkan terjadinya kerusakan permeabilitas dinding sel bakteri, mikrosom, dan lisosom sebagai hasil interaksi antara flavonoid dengan DNA bakteri, sedangkan alkaloid memiliki kemampuan sebagai anti-bakteri. Mekanisme yang diduga adalah dengan cara mengganggu komponen penyusun peptidoglikan pada sel bakteri, sehingga lapisan dinding sel tidak terbentuk secara utuh dan mengakibatkan kematian sel (Dewi dan Fawzya, 2006;Alcorlo et al, 2017;Neelay et al, 2017;Bougault et al, 2018;Caveney et al, 2018).…”

Section: Hasil Dan Pembahasanunclassified

Pemanfaatan Kitosan Kulit Udang Dengan Penambahan Ekstrak Daun Kesum Sebagai Penghambat Bakteri Pada Edible Coating

Lestari

Munir

Tribudi

2018

JTP

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ABSTRAKPenelitian bertujuan untuk mengetahui pengaruh daya hambat edible coating kitosan -pati biji durian dengan penambahan ekstrak daun kesum terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan perlakuan konsentrasi ekstrak daun kesum K 0 =0.00%; K 1 =0.20%; K 2 =0.40%; K 3 =0.60%; K 4 =0.80%; K 5 =1.00%; K 6 =1.20%, dan K 7 =1.40%. Edible coating dibuat dengan formula dasar pati biji durian 3.00%; kitosan 0.80%; gliserol 0.60%. Parameter yang diamati adalah daya hambat menggunakan metode paper disk. Hasil penelitian menunjukan bahwa daya hambat edible coating pati biji durian -kitosan dengan penambahan ekstrak daun kesum berpengaruh sangat nyata (p<0.01) terhadap pertumbuhan bakteri Staphylococcus aureus maupun Escherichia coli. Nilai daya hambat edible coating dengan penambahan ekstrak daun kesum terhadap bakteri Staphylococcus aureus antara 3.432 -4.344 mm, sedangkan bakteri Escherichia coli antara 2.233-3.596 mm. Daya hambat terhadap bakteri yang tertinggi adalah ekstrak daun kesum 1.40% sebesar 4.344 mm (Staphylococcus aureus) dan 3.596 mm (Escherichia coli). Semakin tinggi konsentrasi ekstrak daun kesum, terbukti bahwa semakin tinggi daya hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli ABSTRACT The aim of this research is to determine the inhibitory effect of edible coating chitosan from durian seed starch by the addition of Kesum leaf extract toward the growth of Staphylococcus aureus and Escherichia coli. This experimental design used is a Completely Randomized Design (CRD) treated by concentration of Kesum leaf extract (K): K 0 =0.00%, K 1 =0.20%, K 2 = 0.40%, K 3 =0.60%, K 4 =0.80%, K 5 =1.00%, K 6 =1.20%, and K 7 =1.40%. The formulation of edible coating consist of 3.00% durian seed starch; 0.80% chitosan and 0.60% gliserol. The observed parameter is inhibitory effect using paper disk method. The result showed that the inhibition of edible coating chitosan from durian seed starch treated with the addition of Kesum leaf extract has significantly effect (p<0.01) on inhibition the growth of Staphylococcus aureus and Escherichia coli. The inhibitory effect of edible coating by adding Kesum leaf extract to S. aereus growth ranged 3. mm. The highest of inhibitory effect on bacteria was 1.40% Kesum leaf extract; 4.344 mm in Staphylococcus aureus and 3.596 mm in Escherichia coli. The higher the concentration of Kesum leaf extract, the greater the inhibition of Staphylococcus aureus and Escherichia coli

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“…Moreover, AMPs, as cationic molecules, can interact not only with the phospholipids of bacterial membranes but also with peptidoglycan, which contains components carrying a negative charge. In fact, such a study describing the direct interaction of AMPs with peptidoglycan was recently published [5].…”

Section: Proceedings Of the 35thmentioning

confidence: 99%

Synergy of short antimicrobial peptides withb -lactam antibiotics against MRSA resides in the degradation of peptidoglycan barrier

Nešuta¹,

Kyznar²,

Volejníková³

et al. 2018

Proceedings of the 35th European Peptide Symposium

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Antimicrobial peptides interact with peptidoglycan

Cited by 24 publications

References 66 publications

Peptidoglycan potentiates the membrane disrupting effect of the carboxyamidated form of DMS-DA6, a Gram-positive selective antimicrobial peptide isolated from Pachymedusa dacnicolor skin

Peptidoglycan potentiates the membrane disrupting effect of the carboxyamidated form of DMS-DA6, a Gram-positive selective antimicrobial peptide isolated from Pachymedusa dacnicolor skin

Pemanfaatan Kitosan Kulit Udang Dengan Penambahan Ekstrak Daun Kesum Sebagai Penghambat Bakteri Pada Edible Coating

Synergy of short antimicrobial peptides withb -lactam antibiotics against MRSA resides in the degradation of peptidoglycan barrier

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