Antimicrobial peptides of the genus Capsicum: a mini review

Oliveira, Arielle Pinheiro Bessiatti Fava; Resende, Larissa Maximiano; Rodrigues, Rosana; Mello, Érica de Oliveira; Taveira, Gabriel Bonan; Carvalho, André de Oliveira; Gomes, Valdirene Moreira

doi:10.1007/s13580-022-00421-x

Cited by 9 publications

(7 citation statements)

References 95 publications

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“…Common examples of cystine knot peptides and Knottin peptide-rich plants commonly taken as food have been summarized in Table 1 ; they include cyclotides from Clitoria ternatea [ 25 ], PA1b peptides from Pisum sativum [ 26 ], knottin-like protease inhibitors from Beta vulgaris [ 13 ], knottin-type Roseltides from Hibiscus sabdariffa [ 27 ], Morintides from Moringa oleifera [ 28 ], knottin-type antimicrobial peptides from Amaranthus spp. [ 12 , 29 ] and antibacterial knottin-like peptides from Ghost pepper [ 30 ] and other Capsicum species recently reviewed by Oliveira and colleagues [ 31 ]. The contributory nutritional role of these knottins in the diets requires deliberate scientific attention.…”

Section: Applications Of Knottin-rich Peptidesmentioning

confidence: 99%

Nutritional and Pharmaceutical Applications of Under-Explored Knottin Peptide-Rich Phytomedicines

Attah

Lawal

Yusuf

et al. 2022

Plants

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Phytomedicines reportedly rich in cystine knot peptides (Knottins) are found in several global diets, food/herbal supplements and functional foods. However, their knottin peptide content has largely been unexplored, notably for their emerging dual potentials at both the food and medicine space. The nutritional roles, biological targets and mechanism(s) of activity of these knotted peptides are largely unknown. Meanwhile, knottins have recently been unveiled as emerging peptide therapeutics and nutraceuticals of primary choice due to their broad spectrum of bioactivity, hyper stability, selective toxicity, impressive selectivity for biomolecular targets, and their bioengineering applications. In addition to their potential dietary benefits, some knottins have displayed desirable limited toxicity to human erythrocytes. In an effort to appraise what has been accomplished, unveil knowledge gaps and explore the future prospects of knottins, an elaborate review of the nutritional and pharmaceutical application of phytomedicines rich in knottins was carried out. Herein, we provide comprehensive data on common dietary and therapeutic knottins, the majority of which are poorly investigated in many food-grade phytomedicines used in different cultures and localities. Findings from this review should stimulate scientific interest to unveil novel dietary knottins and knottin-rich nutraceutical peptide drug candidates/leads with potential for future clinical application.

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Section: Applications Of Knottin-rich Peptidesmentioning

confidence: 99%

Nutritional and Pharmaceutical Applications of Under-Explored Knottin Peptide-Rich Phytomedicines

Attah

Lawal

Yusuf

et al. 2022

Plants

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“…Over the last few years, our research group has isolated and characterized antimicrobial peptides present in seeds of different plant species, which have shown inhibitory activity on proteases, especially serine proteases. In plants of the genus Capsicum, peptides characterized as inhibitors of trypsin and chymotrypsin-protease with antifungal activity (MIC 50-250 μg.mL -1 ) were identified, mainly in C. annuum and C. chinense [10,11]. The antifungal activity of these AMPs was characterized by visualization of cell agglomeration and pseudohyphae formation or by hyphal morphological changes as well as by membrane permeabilization due to reactive oxygen species (ROS) induction [11,12].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Antimicrobial Activity of Protease and α-Amylase Inhibitors from Immature Fruits of Capsicum chinense Jacq.

Bramusse,

Silva,

Santos

et al. 2024

Braz. arch. biol. technol.

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Antimicrobial peptides (AMPs) are small groups of proteins obtained from plants and animals. AMPs participate in the immune response, as they provide a quick line of defense against infections, while others may be related to the plant's defense against certain pests and pathogens. The objective of the present study was to evaluate an inhibitory activity of fractions obtained from immature fruits of Capsicum chinense Jacq. (accession UENF 1755) on trypsin, chymotrypsin and α-amylase families and on yeast growth. The peptides were obtained from the immature fruits using saline extraction. The extract was semipurified by DEAE-Sepharose chromatography into two fractions: D1 (non-retained fraction) and D2 (retained fraction), and analyzed using SDS-tricine-gel electrophoresis. The antifungal activity of these fractions was tested on Candida albicans, Candida buinensis, Candida parapsilosis and Candida tropicalis. To elucidate the antimicrobial mechanism of these fractions, membrane permeabilization and endogenous reactive oxygen species (ROS) induction assays were performed. The fractions were also tested for inhibition of trypsin, chymotrypsin and α-amylase enzymes. The two fractions, D1 and D2, inhibited yeast HIGHLIGHTS• Immature fruits of C. chinense presents AMPs with antifungal activity against yeasts.• D1 fraction has greater antifungal activity.• D1 fraction shows activity against C. albicans and C. tropicalis, two yeasts of medical interest.

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“…17 The pepper genus Capsicum is a promising source of AMPs, including defensins, with a wide range of inhibitory actions. [18][19][20] However, few investigations have been conducted to identify and analyze defensins in Capsicum chinense. In previous studies, Resende et al, 21 obtained a fraction rich in peptides called the G3 fraction, which inhibited the growth of fungi of the genera Colletotrichum and Fusarium.…”

Section: Introductionmentioning

confidence: 99%

“…The pepper genus Capsicum is a promising source of AMPs, including defensins, with a wide range of inhibitory actions 18–20 . However, few investigations have been conducted to identify and analyze defensins in Capsicum chinense .…”

Section: Introductionmentioning

confidence: 99%

Defensin‐like peptides from Capsicum chinense induce increased ROS, loss of mitochondrial functionality, and reduced growth of the fungus Colletotrichum scovillei

Resende,

de Oliveira Mello,

Zeraik

et al. 2024

Pest Management Science

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In the present study, we identified and characterized two defensin‐like peptides in an antifungal fraction obtained from Capsicum chinense pepper fruits and inhibited the growth of Colletotrichum scovillei, which causes anthracnose. AMPs were extracted from the pericarp of C. chinense peppers and subjected to ion exchange, molecular exclusion, and reversed‐phase in a high‐performance liquid chromatography system. We investigated the endogenous increase in reactive oxygen species (ROS), the loss of mitochondrial functioning, and the ultrastructure of hyphae. The peptides obtained from the G3 fraction through molecular exclusion chromatography were subsequently fractionated using reverse‐phase chromatography, resulting in the isolation of fractions F1, F2, F3, F4, and F5. The F1 fraction suppressed C. scovillei growth by 90, 70.4, and 44% at 100, 50, and 25 μg/mL, respectively. At 24 h, the IC50 and minimum inhibitory concentration were 21.5 μg/mL and 200 μg/mL, respectively. We found an increase in ROS, which may have resulted in an oxidative burst, loss of mitochondrial functioning, and cytoplasm retraction, as well as an increase in autophagic vacuoles. MS/MS analysis of the F1 fraction indicated the presence of two defensin‐like proteins, and we were able to identify the expression of three defensin sequences in our C. chinense fruit extract. The F1 fraction was also found to inhibit the activity of insect α‐amylases. In summary, the F1 fraction of C. chinense exhibits antifungal activity against a major pepper pathogen that causes anthracnose. These defensin‐like compounds are promising prospects for further research into antifungal and insecticide biotechnology applications.This article is protected by copyright. All rights reserved.

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Antimicrobial peptides of the genus Capsicum: a mini review

Cited by 9 publications

References 95 publications

Nutritional and Pharmaceutical Applications of Under-Explored Knottin Peptide-Rich Phytomedicines

Nutritional and Pharmaceutical Applications of Under-Explored Knottin Peptide-Rich Phytomedicines

Characterization and Antimicrobial Activity of Protease and α-Amylase Inhibitors from Immature Fruits of Capsicum chinense Jacq.

Defensin‐like peptides from Capsicum chinense induce increased ROS, loss of mitochondrial functionality, and reduced growth of the fungus Colletotrichum scovillei

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