Antimicrobial Peptoids Are Effective against Pseudomonas aeruginosa Biofilms

Kapoor, Rinki; Wadman, Mayken W.; Dohm, Michelle T.; Czyzewski, Ann M.; Spormann, Alfred M.; Barron, Annelise E.

doi:10.1128/aac.01516-10

Cited by 119 publications

(101 citation statements)

References 29 publications

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“…It is thought that the peptoid 1-C134mer, which has an hydrophobic tail and is active against P. aeruginosa biofilms, interacts strongly with and disrupt the hydrophobic matrix due to its surfactant like nature, facilitating deeper penetration (Kapoor et al 2011).…”

Section: Anti-biofilm Antimicrobial Peptide Strategiesmentioning

confidence: 99%

New trends in peptide-based anti-biofilm strategies: a review of recent achievements and bioinformatic approaches

2012

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Antimicrobial peptides (AMPs) have a broad spectrum of activity and unspecific mechanisms of action. Therefore, they are seen as valid alternatives to overcome clinically relevant biofilms and reduce the chance of acquired resistance. This paper reviews AMPs and anti-biofilm AMP-based strategies and discusses ongoing and future work. Recent studies report successful AMP-based prophylactic and therapeutic strategies, several databases catalogue AMP information and analysis tools, and novel bioinformatics tools are supporting AMP discovery and design. However, most AMP studies are performed with planktonic cultures, and most studies on sessile cells test AMPs on growing rather than mature biofilms. Promising preliminary synergistic studies have to be consubstantiated and the study of functionalized coatings with AMPs must be further explored. Standardized operating protocols, to enforce the repeatability and reproducibility of AMP anti-biofilm tests, and automated means of screening and processing the ever-expanding literature are still missing.

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Section: Anti-biofilm Antimicrobial Peptide Strategiesmentioning

confidence: 99%

New trends in peptide-based anti-biofilm strategies: a review of recent achievements and bioinformatic approaches

2012

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“…For the most part of the last decade, Methicillin-resistant Staphylococcus aureus (MRSA) was the leading cause of HAIs in Ireland [3]. However, another bacterium, Pseudomonas aeruginosa, is of increasing concern worldwide with for example 200,000 HAIs directly related to that organism annually in the USA alone [4]. Bacteria such P. aeruginosa, Staphylococci and Escherichia coli that can exist in biofilms exhibit extreme resistance compared to planktonic or free moving bacteria as they are protected from the host's immune cells and from the action of antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterisation of silver(I) complexes of substituted coumarin-4-carboxylates which are effective against Pseudomonas aeruginosa biofilms

et al. 2014

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a b s t r a c tA novel series of coumarin-4-carboxylate ligands (2a-2l), their Ag(I) complexes (3a-3l) together with a number of their phenanthroline adducts (4d, 4f and 4g) have been synthesised and characterised. The ligands were prepared by either acid or base hydrolysis of their corresponding esters or by demethylation of a methylated acid derivative. The esters (1a-1i) were synthesised by an aromatic electrophilic substitution reaction between the conjugate base of substituted phenols and a vinyltriphenylphosphonium salt. The novel series of Ag(I) carboxylate complexes were prepared by reaction of silver nitrate with the coumarin ligands in aqueous/alcohol solution. The Ag(I) phenanthroline adducts were prepared by reaction of the Ag(I) complex with 1,10-phenanthroline in aqueous/alcohol solution. A selection of complexes were screened for their in vitro antibacterial activity against Pseudomonas aeruginosa grown planktonically or as a biofilm as well as a number of other clinically important strains, Escherichia coli, MRSA and Staphylococcus aureus. The activity against the P. aeruginosa biofilm was found to be significantly greater than against the planktonic bacteria.

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“…Therefore, we have developed mimics of AMPs using peptoids (poly-N-substituted glycines), which are protease-resistant (15) isomers of peptides ( Fig. 1) with broad-spectrum antibacterial activity comparable to, and in some cases better than, that of antimicrobial peptides (4,5,11,12,19).…”

mentioning

confidence: 99%

Functional Synergy between Antimicrobial Peptoids and Peptides against Gram-Negative Bacteria

Chongsiriwatana

Wetzler

Barron

2011

Antimicrob Agents Chemother

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Antimicrobial peptides (AMPs) are integral components of innate immunity and are typically found in combinations in which they can synergize for broader-spectrum or more potent activity. Previously, we reported peptoid mimics of AMPs with potent and selective antimicrobial activity. Using checkerboard assays, we demonstrate that peptoids and AMPs can interact synergistically, with fractional inhibitory concentration indices as low as 0.16. These results strongly suggest that antimicrobial peptoids and peptides are functionally and mechanistically analogous.

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Antimicrobial Peptoids Are Effective against Pseudomonas aeruginosa Biofilms

Cited by 119 publications

References 29 publications

New trends in peptide-based anti-biofilm strategies: a review of recent achievements and bioinformatic approaches

New trends in peptide-based anti-biofilm strategies: a review of recent achievements and bioinformatic approaches

Isolation and characterisation of silver(I) complexes of substituted coumarin-4-carboxylates which are effective against Pseudomonas aeruginosa biofilms

Functional Synergy between Antimicrobial Peptoids and Peptides against Gram-Negative Bacteria

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