Antimicrobial photodynamic activity of rose bengal conjugated multi walled carbon nanotubes against planktonic cells and biofilm of Escherichia coli

Vt, Anju; Parasuraman, Paramanantham; Sb, Sruthil Lal; Sharan, Alok; Alsaedi, Marzouq H.; Dawoud, Turki M.; Asad, Syed Muhammad; Siddhardha, Busi

doi:10.1016/j.pdpdt.2018.10.013

Cited by 41 publications

(14 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antibacterial activity of RB via photodynamic approaches has been studied in several research groups [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. Applications of photodynamic therapy of RB are not limited to skin infections, including cellulitis, erysipelas, impetigo, folliculitis, and furuncles and carbuncles.…”

Section: Resultsmentioning

confidence: 99%

“…Rose bengal (RB) dye (4,5,6,7-tetrachloro-2′,4′,5′,7′-tetraiodofluorescein) has been clinically investigated for the treatment of melanoma and other solid cancers [ 10 , 11 , 12 , 13 , 14 ]. Photodynamic antibacterial properties of RB have been reported for a limited number of bacteria [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. RB is a bright rose-red xanthene compound that was first synthesized in the 19th century as a wool dye and subsequently used as a food dye in Japan (food red no.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antibacterial Activity of Pharmaceutical-Grade Rose Bengal: An Application of a Synthetic Dye in Antibacterial Therapies

et al. 2022

View full text Add to dashboard Cite

Rose bengal has been used in the diagnosis of ophthalmic disorders and liver function, and has been studied for the treatment of solid tumor cancers. To date, the antibacterial activity of rose bengal has been sporadically reported; however, these data have been generated with a commercial grade of rose bengal, which contains major uncontrolled impurities generated by the manufacturing process (80–95% dye content). A high-purity form of rose bengal formulation (HP-RBf, >99.5% dye content) kills a battery of Gram-positive bacteria, including drug-resistant strains at low concentrations (0.01–3.13 μg/mL) under fluorescent, LED, and natural light in a few minutes. Significantly, HP-RBf effectively eradicates Gram-positive bacterial biofilms. The frequency that Gram-positive bacteria spontaneously developed resistance to HP-RB is extremely low (less than 1 × 10−13). Toxicity data obtained through our research programs indicate that HP-RB is feasible as an anti-infective drug for the treatment of skin and soft tissue infections (SSTIs) involving multidrug-resistant (MDR) microbial invasion of the skin, and for eradicating biofilms. This article summarizes the antibacterial activity of pharmaceutical-grade rose bengal, HP-RB, against Gram-positive bacteria, its cytotoxicity against skin cells under illumination conditions, and mechanistic insights into rose bengal’s bactericidal activity under dark conditions.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Pharmaceutical-Grade Rose Bengal: An Application of a Synthetic Dye in Antibacterial Therapies

et al. 2022

View full text Add to dashboard Cite

show abstract

“…where P = the radiant flux of the laser used for exposure in Watts (W) and A = the irradiated area in cm 2 (π r 2 ) 18…”

Section: Methodsmentioning

confidence: 99%

“…Owing to their sp 2 -hybridized carbon surfaces and large surface area, CNTs have a high-loading capacity (LC) for the delivery of several payloads 17. Researchers have reported CNTs conjugated with rose bengal for maximum antimicrobial photodynamic destruction of E. coli biofilms, wherein CNTs act as a dye carrier 18. In another study, single-walled-CNT-aminoporphyrin conjugates were synthesized for the efficient photodynamic inactivation of Gram-positive bacteria 19.…”

Section: Introductionmentioning

confidence: 99%

Malachite green-conjugated multi-walled carbon nanotubes potentiate antimicrobial photodynamic inactivation of planktonic cells and biofilms of Pseudomonas aeruginosa and Staphylococcus aureus

Anju¹,

Parasuraman²,

Siddhardha³

et al. 2019

IJN

Self Cite

View full text Add to dashboard Cite

Purpose: Infections associated with medical devices that are caused by biofilms remain a considerable challenge for health care systems owing to their multidrug resistance patterns. Biofilms of Pseudomonas aeruginosa and Staphylococcus aureus can result in life-threatening situations which are tough to eliminate by traditional methods. Antimicrobial photodynamic inactivation (aPDT) constitutes an alternative method of killing deadly pathogens and their biofilms using reactive oxygen species (ROS). This study investigated the efficacy of enhanced in vitro aPDT of P. aeruginosa and S. aureus using malachite green conjugated to carboxyl-functionalized multi-walled carbon nanotubes (MGCNT). Both the planktonic cells and biofilms of test bacteria were demonstrated to be susceptible to the MGCNT conjugate. These MGCNT conjugates may thus be employed as a facile strategy for designing antibacterial and anti-biofilm coatings to prevent the infections associated with medical devices. Methods: Conjugation of the cationic dye malachite green to carbon nanotube was studied by UV-visible spectroscopy, high-resolution transmission electron microscopy, and Fourier transform infrared spectrometry. P. aeruginosa and S. aureus photodestruction were studied using MGCNT conjugate irradiated for 3 mins with a red laser of wavelength 660 nm and radiant exposure of 58.49 J cm −2 . Results: Upon MGCNT treatment, S. aureus and P. aeruginosa were reduced by 5.16 and 5.55 log 10 , respectively. Compared to free dye, treatment with MGCNT afforded improved phototoxicity against test bacteria, concomitant with greater ROS production. The results revealed improved biofilm inhibition, exopolysaccharide inhibition, and reduced cell viability in test bacteria treated with MGCNT conjugate. P. aeruginosa and S. aureus biofilms were considerably reduced to 60.20±2.48% and 67.59±3.53%, respectively. Enhanced relative MGCNT phototoxicity in test bacteria was confirmed using confocal laser scanning microscopy. Conclusion: The findings indicated that MGCNT conjugate could be useful to eliminate the biofilms formed on medical devices by S. aureus and P. aeruginosa.

show abstract

“…The potential broad-spectrum antimicrobial action of carbon nanotubes also enhances their efficacy of antibacterial therapy [139] . In recent studies, RB conjugated to MWCNT [140] or MB conjugated to SWCNT [139] , evidenced an enhanced photodynamic efficiency against E. coli, disregard the charge of the PS, and due to a greater interaction with bacteria. In the same line, Yu et al reported in 2019 a supramolecular self-assembly of a polycationic porphyrin (168, Fig.…”

Section: Other Carbon-based Materialsmentioning

confidence: 99%

Latest trends on photodynamic disinfection of Gram-negative bacteria: photosensitizer’s structure and delivery systems

Maldonado-Carmona

Ouk

Leroy‐Lhez

2021

Photochem Photobiol Sci

View full text Add to dashboard Cite

Antimicrobial photodynamic activity of rose bengal conjugated multi walled carbon nanotubes against planktonic cells and biofilm of Escherichia coli

Cited by 41 publications

References 41 publications

Antibacterial Activity of Pharmaceutical-Grade Rose Bengal: An Application of a Synthetic Dye in Antibacterial Therapies

Antibacterial Activity of Pharmaceutical-Grade Rose Bengal: An Application of a Synthetic Dye in Antibacterial Therapies

<p>Malachite green-conjugated multi-walled carbon nanotubes potentiate antimicrobial photodynamic inactivation of planktonic cells and biofilms of <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em></p>

Latest trends on photodynamic disinfection of Gram-negative bacteria: photosensitizer’s structure and delivery systems

Contact Info

Product

Resources

About