Antimicrobial photodynamic therapy effectively reduces Porphyromonas gingivalis infection in gingival fibroblasts and keratinocytes: An in vitro study

Oruba, Zuzanna; Gawron, Katarzyna; Bereta, Grzegorz; Sroka, Aneta; Potempa, Jan; Chomyszyn‐Gajewska, Maria

doi:10.1016/j.pdpdt.2021.102330

Cited by 9 publications

(12 citation statements)

References 40 publications

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“…Enhanced periodontal regeneration by PDLSCs implantation has been achieved with and without a scaffold 29,30 . The underlying mechanisms are attributed to the osteo/dentinogenic differentiation potential of stem cells, the effect of inflammation inhibition (such as downregulation of TNFα and IL‐1β), the immunomodulatory capacities on macrophages and T cells, and the paracrine effect of various factors, including anti‐apoptosis, immunomodulation, and anti‐inflammation effects 32 . PC‐SPIO achieved better regeneration results than PC alone, which probably reflected the stronger osteogenic differentiation capacity of PC‐SPIO compared with PC.…”

Section: Discussionmentioning

confidence: 99%

An altered oral microbiota induced by injections of superparamagnetic iron oxide nanoparticle‐labeled periodontal ligament stem cells helps periodontal bone regeneration in rats

Shi

Liang

Zhang

et al. 2022

Bioengineering & Transla Med

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Stem cell injection is good for periodontal regeneration due to the capacity of stem cells to differentiate toward osteogenic direction and to regulate the production of pro‐ and anti‐inflammatory cytokines. However, injected cells are difficult to track in vivo. And there is microbiota in oral cavity, the dysbiosis of which leads to the damage and loss of periodontal tissue. Here, we demonstrated an enhanced periodontal repair was due to an altered oral microbiota. Periodontal defects were surgically prepared in rats, and periodontal ligament stem cells (PDLSCs) labeled by superparamagnetic iron oxide (SPIO) nanoparticles (PC‐SPIO) were injected, with PDLSCs and saline treatments as controls. Detected by magnetic resonance imaging (MRI) and histological staining, PC‐SPIO was major at limited areas in regenerated periodontal tissues. PC‐SPIO‐treated rats achieved better periodontal regeneration than the other two groups. Concurrently, the oral microbiota of PC‐SPIO‐treated rats was changed, presenting SPIO‐Lac as a biomarker. SPIO‐Lac assisted periodontal repair in vivo, inhibited the inflammation of macrophages induced by lipopolysaccharide (LPS) and antibacterial in vitro. Therefore, our study proved that SPIO‐labeled cells can be tracked in periodontal defect and highlighted a potential positive role of an oral microbiota in periodontal regeneration, suggesting the possibility of periodontal repair promotion by manipulating oral microbiota.

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Section: Discussionmentioning

confidence: 99%

An altered oral microbiota induced by injections of superparamagnetic iron oxide nanoparticle‐labeled periodontal ligament stem cells helps periodontal bone regeneration in rats

Shi

Liang

Zhang

et al. 2022

Bioengineering & Transla Med

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“…In-vitro studies have focused on the susceptibility of different periodontal disease-related microorganisms to aPDT. The application of aPDT has been shown to reduce several periodontal disease-associated bacteria such as P. gingivalis, A. actinomycetemcomitans , and Fusobacterium nucleatum (F. nucleatum) in biofilms ( Prasanth et al, 2014 ; Kranz et al, 2015 ; Yoshida et al, 2017 ; Valle et al, 2019 ; Oruba et al, 2021 ). Kikuchi et al’s review confirmed the susceptibility of bacteria to aPDT in different forms of planktonic cultures, plaque scrapings, and biofilms.…”

Section: Antimicrobial Photodynamic Therapy and Periodontal And Peri-...mentioning

confidence: 99%

“…A considerable number of studies have been conducted on the effects of PDT on microorganisms involved with oral diseases, including Porphyromonas gingivalis ( P. gingivalis ; Ding et al, 2021 ; Ghazi et al, 2021 ; Oruba et al, 2021 ; Yoshida et al, 2021 ) as a gram-negative obligate anaerobe bacterium ( Hajishengallis et al, 2012 ) which is the main representative of pathogenic bacteria involved in periodontal diseases ( Ding et al, 2021 ), Streptococcus mutans ( S. mutans ; Benine-Warlet et al, 2022 ; Dos Santos et al, 2022 ; Fernandes et al, 2022 ) which is the main bacteria involved in caries development, and Candida albicans which is the main opportunistic fungus in the oral microbial flora ( de Lapena et al, 2022 ; Dos Santos et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Clinical applications of antimicrobial photodynamic therapy in dentistry

et al. 2023

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Given the emergence of resistant bacterial strains and novel microorganisms that globally threaten human life, moving toward new treatment modalities for microbial infections has become a priority more than ever. Antimicrobial photodynamic therapy (aPDT) has been introduced as a promising and non-invasive local and adjuvant treatment in several oral infectious diseases. Its efficacy for elimination of bacterial, fungal, and viral infections and key pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Candida albicans, and Enterococcus faecalis have been investigated by many invitro and clinical studies. Researchers have also investigated methods of increasing the efficacy of such treatment modalities by amazing developments in the production of natural, nano based, and targeted photosensitizers. As clinical studies have an important role in paving the way towards evidence-based applications in oral infection treatment by this method, the current review aimed to provide an overall view of potential clinical applications in this field and summarize the data of available randomized controlled clinical studies conducted on the applications of aPDT in dentistry and investigate its future horizons in the dental practice. Four databases including PubMed (Medline), Web of Science, Scopus and Embase were searched up to September 2022 to retrieve related clinical studies. There are several clinical studies reporting aPDT as an effective adjunctive treatment modality capable of reducing pathogenic bacterial loads in periodontal and peri-implant, and persistent endodontic infections. Clinical evidence also reveals a therapeutic potential for aPDT in prevention and reduction of cariogenic organisms and treatment of infections with fungal or viral origins, however, the number of randomized clinical studies in these groups are much less. Altogether, various photosensitizers have been used and it is still not possible to recommend specific irradiation parameters due to heterogenicity among studies. Reaching effective clinical protocols and parameters of this treatment is difficult and requires further high quality randomized controlled trials focusing on specific PS and irradiation parameters that have shown to have clinical efficacy and are able to reduce pathogenic bacterial loads with sufficient follow-up periods.

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“…[43] Owing to its high binding affinity towards both Gram-positive and Gramnegative bacteria, phenothiazinium-based photosensitizers were frequently used in the research of APDT. [44][45][46] Examples of phenothiazinium compounds include Rose Bengal, [47,48] toluidine blue O [49,50] and methylene blue. [51][52][53] Methylene Blue (MB) is one of the two most popular phenothiazinium dyes in this category that has been commonly used in antimicrobial research.…”

Section: Phenothiaziniummentioning

confidence: 99%

Antimicrobial Photodynamic Therapy for the Remote Eradication of Bacteria

Xin

Kwek

et al. 2023

ChemPlusChem

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The emergence of multi‐drug resistant bacteria strains has been an uphill battle in modern healthcare worldwide, due to the increasing difficulty of killing them. The evolving pathogenicity of bacteria has led to researchers searching for more effective antimicrobial therapeutics to successfully eliminate them without undesirable consequences to the human body. In recent years, antimicrobial photodynamic therapy (APDT), an obsolete technique for cancer treatments, has been reported to eradicate bacteria and biofilm‐related infections. The principle of antimicrobial photodynamic therapy solely relies on the photosensitizers (PSs) generating reactive oxygen species, in the presence of oxygen and light, to destroy pathogens. Thus, it can target a broad spectrum of microorganisms, owing to the indirect interaction between PSs and the bacteria, resulting in the less likelihood for the development of drug resistant bacteria strains. This review will focus on the recent progress of APDT in the last five years and some future perspectives of APDT. The mechanism of APDT against bacteria and biofilms, various PSs used for APDT, and some common multidrug‐resistant bacteria strains will be briefly introduced. The reported in vivo applications of APDT in the several types of bacterial infections that includes periodontitis, wound infections, keratitis, endophthalmitis and tuberculosis in the last five years will be summarized in detail.

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Antimicrobial photodynamic therapy effectively reduces Porphyromonas gingivalis infection in gingival fibroblasts and keratinocytes: An in vitro study

Cited by 9 publications

References 40 publications

An altered oral microbiota induced by injections of superparamagnetic iron oxide nanoparticle‐labeled periodontal ligament stem cells helps periodontal bone regeneration in rats

An altered oral microbiota induced by injections of superparamagnetic iron oxide nanoparticle‐labeled periodontal ligament stem cells helps periodontal bone regeneration in rats

Clinical applications of antimicrobial photodynamic therapy in dentistry

Antimicrobial Photodynamic Therapy for the Remote Eradication of Bacteria

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