Antimicrobial photodynamic therapy on Candida albicans pre-treated by fluconazole delayed yeast inactivation

Ferreira, Luis Rodolfo; Sousa, Aline Silva; Alvarenga, Letícia Heineck; Deana, Alessandro Melo; Santi, Maria Eugênia Onofre Simões de; Kato, Ilka Tiemy; Leal, Cintia Raquel Lima; Ribeiro, Martha S.; Prates, Renato Araújo

doi:10.1016/j.pdpdt.2016.05.002

Cited by 13 publications

(6 citation statements)

References 11 publications

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“…In this experiment, C. albicans suspension was treated with fluconazole followed by PACT using 1. The use of the antifungal resulted in delaying the complete inactivation of the yeast [66]. These results are in obvious opposition to those quoted earlier, but the authors do not attempt to explain the phenomenon unequivocally.…”

Section: Combination Of Phenothiazines With Antifungalscontrasting

confidence: 70%

“…The authors suggest that this may be related to the antifungal-induced oxidative stress, thus enhancing the formation of ROS [65]. Other quite surprising study results were reported in 2016 by Ferreira et al [66]. In this experiment, C. albicans suspension was treated with fluconazole followed by PACT using 1.…”

Section: Combination Of Phenothiazines With Antifungalsmentioning

confidence: 69%

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Photosensitizers Mediated Photodynamic Inactivation against Fungi

Ziental

Mlynarczyk

Czarczyńska-Goślińska

et al. 2021

Nanomaterials

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Superficial and systemic fungal infections are essential problems for the modern health care system. One of the challenges is the growing resistance of fungi to classic antifungals and the constantly increasing cost of therapy. These factors force the scientific world to intensify the search for alternative and more effective methods of treatment. This paper presents an overview of new fungal inactivation methods using Photodynamic Antimicrobial Chemotherapy (PACT). The results of research on compounds from the groups of phenothiazines, xanthanes, porphyrins, chlorins, porphyrazines, and phthalocyanines are presented. An intensive search for a photosensitizer with excellent properties is currently underway. The formulation based on the existing ones is also developed by combining them with nanoparticles and common antifungal therapy. Numerous studies indicate that fungi do not form any specific defense mechanism against PACT, which deems it a promising therapeutic alternative.

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Section: Combination Of Phenothiazines With Antifungalscontrasting

confidence: 70%

Section: Combination Of Phenothiazines With Antifungalsmentioning

confidence: 69%

Photosensitizers Mediated Photodynamic Inactivation against Fungi

Ziental

Mlynarczyk

Czarczyńska-Goślińska

et al. 2021

Nanomaterials

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“…It has known potential and competence for inhibiting the synthesis of ergosterol, a main component in the fungal cell membrane, curing fungal infections like candidiasis, cryptococcal meningitis, and dermatophytic fungal infections like aspergillosis, coccidioidomycosis, histoplasmosis, and also for preventing their recurrences. It also prevents yeast infections in patients undergoing treatment for cancer and acquired immune-deficiency syndrome (AIDS). , However, due to the low aqueous solubility of this multifunction drug, the need arises for improving its physicochemical properties using CE strategies. Different research studies have reported successful breakthroughs in synthesizing different pharmaceutical solid forms of FLZ.…”

Section: Introductionmentioning

confidence: 99%

“…It also prevents yeast infections in patients undergoing treatment for cancer and acquired immune-deficiency syndrome (AIDS). 30,31 However, due to the low aqueous solubility of this multifunction drug, the need arises for improving its physicochemical properties using CE strategies. Different research studies have reported successful breakthroughs in synthesizing different pharmaceutical solid forms of FLZ.…”

Section: Introductionmentioning

confidence: 99%

Fluconazole: Synthesis and Structural Characterization of Four New Pharmaceutical Cocrystal Forms

Owoyemi

Silva

Souza

et al. 2019

Crystal Growth & Design

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Pharmaceutical cocrystals have emerged over the past several decades as an alternative path for synthesizing stable and/or improved crystalline forms of active pharmaceutical ingredients. In this contribution, we developed a reproducible cocrystallization path for the supramolecular synthesis of four new pharmaceutical cocrystal forms of fluconazole (FLZ), an antifungal multifunctional drug: fluconazole–fumaric acid monohydrate (1:1:1), fluconazole–malic acid (1:1), fluconazole–dipicolinic acid (1:1), and fluconazole–adipic acid (1:1). All the new cocrystals were characterized by powder/single-crystal X-ray diffraction, Raman, Fourier transform infrared spectroscopy, differential scanning calorimetry/thermogravimetric analysis, and hot-stage polarized optical microscopy, and their water solubility was determined. Structurally, although the coformers were different, the same strong O–H···N hydrogen bond between the FLZ molecule and the coformers was observed. The aqueous solubility studies revealed that all the cocrystals were found to exhibit improved aqueous solubility when compared to the commercialized FLZ polymorph.

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“…Ferreira et al and de Oliveira-Silva et al achieved different reductions of C. albicans , 0.5 log 10 and 6 log 10 respectively, with 32 μg/mL of MB and a fluence of 30 J/cm 2 for 3–4 min. This shows the variability between experiments [ 29 , 30 ]. The results of Ferreira et al are closer to those of this work (32 μg/mL of MB does not produce complete photoinactivation) ( Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effect of Photodynamic Therapy with Different Lights and Combined or Uncombined with Chlorhexidine on Candida spp.

et al. 2021

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Candidiasis is very common and complicated to treat in some cases due to increased resistance to antifungals. Antimicrobial photodynamic therapy (aPDT) is a promising alternative treatment. It is based on the principle that light of a specific wavelength activates a photosensitizer molecule resulting in the generation of reactive oxygen species that are able to kill pathogens. The aim here is the in vitro photoinactivation of three strains of Candida spp., Candida albicans ATCC 10231, Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258, using aPDT with different sources of irradiation and the photosensitizer methylene blue (MB), alone or in combination with chlorhexidine (CHX). Irradiation was carried out at a fluence of 18 J/cm2 with a light-emitting diode (LED) lamp emitting in red (625 nm) or a white metal halide lamp (WMH) that emits at broad-spectrum white light (420–700 nm). After the photodynamic treatment, the antimicrobial effect is evaluated by counting colony forming units (CFU). MB-aPDT produces a 6 log10 reduction in the number of CFU/100 μL of Candida spp., and the combination with CHX enhances the effect of photoinactivation (effect achieved with lower concentration of MB). Both lamps have similar efficiencies, but the WMH lamp is slightly more efficient. This work opens the doors to a possible clinical application of the combination for resistant or persistent forms of Candida infections.

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Antimicrobial photodynamic therapy on Candida albicans pre-treated by fluconazole delayed yeast inactivation

Cited by 13 publications

References 11 publications

Photosensitizers Mediated Photodynamic Inactivation against Fungi

Photosensitizers Mediated Photodynamic Inactivation against Fungi

Fluconazole: Synthesis and Structural Characterization of Four New Pharmaceutical Cocrystal Forms

In Vitro Effect of Photodynamic Therapy with Different Lights and Combined or Uncombined with Chlorhexidine on Candida spp.

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