Antimicrobial Photodynamic Therapy with Diode laser and Methylene blue as an adjunct to scaling and root planning: A clinical trial

Derikvand, Nahid; Ghasemi, Seyedeh Sara; Safiaghdam, Hannaneh; Piriaei, Hassan; Chiniforush, Nasim

doi:10.1016/j.pdpdt.2020.101818

Cited by 22 publications

(16 citation statements)

References 28 publications

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“…aPDT has been developed as a promising, non-invasive treatment method in treating some skin-related cancers, age-related macular degeneration and infectious diseases. [19,20] aPDT also is mentioned as an adjunct for periodontal therapy as it kills periodontal pathogens or might decrease their virulence, so help clinicians to improve treatment outcomes. [21] Gharesi et al [22] investigated the effect of aPDT based on ICG with an 808-nm diode laser on apoptosis-related genes expression in human gingival fibroblasts (HGF) as they play a role in the periodontal tissues repair and regeneration.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of indocyanine-mediated photodynamic therapy cytotoxicity in human osteoblast-like cells: an in vitro study

Aghayan¹,

Yazdanfar²,

Seyedjafari³

et al. 2022

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Introduction: Antimicrobial photodynamic therapy (aPDT) is an adjunctive non-invasive procedure for the management of periodontal tissue infection and deep periodontal pocket decontamination. However, the effects of this procedure on periodontal cells like osteoblasts that play a role in periodontal tissue repair and regeneration is not yet clear. Aim: This study aimed to investigate aPDT based on indocyanine green (ICG) on MG-63 osteoblast-like cells in vitro. Materials and methods: MG-63 cells were treated in 9 different groups: 1) Control (untreated cells), 2) ICG alone at a concentration of 1000 µg/mL, 3) ICG alone at a concentration of 2000 µg/mL, 4) diode laser irradiation alone for 30 s, 5) laser irradiation for 30 s combined with ICG with a concentration of 1000 µg/mL, 6) laser irradiation for 30 s combined with ICG at a concentration of 2000 µg/ mL, 7) laser irradiation alone for 60 s, 8) laser irradiation for 60 s combined with ICG at a concentration of 1000 µg/mL, and 9) laser irradiation for 60 s combined with ICG at a concentration of 2000 µg/mL. Cell viability was assessed by MTT assay in different groups. Results: In groups that were treated with 2000 µg/mL of ICG or diode laser irradiation at fluency of 39 J/cm2 for 60 s alone or in combination during ICG-aPDT, osteoblast-like cells viability decreased, remarkably. Conclusions: IGC-mediated aPDT with 1000 µg/mL of ICG combined with diode laser irradiation at fluency of 39 J/cm2 for 30 s is safe for MG-63 human osteoblast-like cells; however, higher concentration of ICG or laser irradiation time will increase cells death. There is still a need for more in vivo studies.

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Section: Discussionmentioning

confidence: 99%

Evaluation of indocyanine-mediated photodynamic therapy cytotoxicity in human osteoblast-like cells: an in vitro study

Aghayan¹,

Yazdanfar²,

Seyedjafari³

et al. 2022

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“…For clinical studies of MB-PDT, concentrations ranging from 25-100 µg/mL have typically been reported [42][43][44] , with some investigators using higher concentrations for topical application 45 .…”

Section: Discussionmentioning

confidence: 99%

First in human measurements of abscess cavity optical properties and methylene blue uptake prior to photodynamic therapy byin vivodiffuse reflectance spectroscopy

Hannan,

Sharma,

Baran

2023

Preprint

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SignificanceEfficacious photodynamic therapy (PDT) of abscess cavities requires personalized treatment planning. This relies on knowledge of abscess wall optical properties, which we report for the first time in human subjects.AimThe objective was to extract optical properties and photosensitizer concentration from spatially-resolved diffuse reflectance measurements of abscess cavities prior to methylene blue (MB) PDT, as part of a Phase 1 clinical trial.ApproachDiffuse reflectance spectra were collected at the abscess wall of 13 human subjects using a custom fiber-optic probe and optical spectroscopy system, before and after MB administration. A Monte Carlo lookup table was used to extract optical properties.ResultsPre-MB abscess wall absorption coefficients at 665 nm were 0.15±0.1 cm−1(0.03-0.36 cm−1) and 10.74±15.81 cm−1(0.08-49.3 cm−1) post-MB. Reduced scattering coefficients at 665 nm were 8.45±2.37 cm−1(4.8-13.2 cm−1) and 5.6±2.26 cm−1(1.6-9.9 cm−1) for pre-MB and post-MB, respectively. Oxygen saturations were found to be 58.83±35.78% (5.6-100%) pre-MB and 36.29±25.1% (0.0001-76.4%) post-MB. Determined MB concentrations were 71.83±108.22 µM (0-311 µM).ConclusionsWe observed substantial inter-subject variation in both native wall optical properties and methylene blue uptake. This underscores the importance of making these measurements for patient-specific treatment planning.

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“…MB is a fluorescent dye that has been investigated for many years as an antibacterial and antifungal substance [ 33 , 34 , 35 ] and antiviral drug in medicine [ 36 , 37 ]. MB generates reactive oxygen species (ROS) in response to light doses lower that 5 J/cm 2 and induces ROS dependent apoptosis in cancer cells, including the A549 lung cancer cell line used in this study [ 30 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Phototherapeutic Induction of Immunogenic Cell Death and CD8+ T Cell-Granzyme B Mediated Cytolysis in Human Lung Cancer Cells and Organoids

Valančiūtė

Mathieson

O’Connor

et al. 2022

Cancers

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Augmenting T cell mediated tumor killing via immunogenic cancer cell death (ICD) is the cornerstone of emerging immunotherapeutic approaches. We investigated the potential of methylene blue photodynamic therapy (MB-PDT) to induce ICD in human lung cancer. Non-Small Cell Lung Cancer (NSCLC) cell lines and primary human lung cancer organoids were evaluated in co-culture killing assays with MB-PDT and light emitting diodes (LEDs). ICD was characterised using immunoblotting, immunofluorescence, flow cytometry and confocal microscopy. Phototherapy with MB treatment and low energy LEDs decreased the proliferation of NSCLC cell lines inducing early necrosis associated with reduced expression of the anti-apoptotic protein, Bcl2 and increased expression of ICD markers, calreticulin (CRT), intercellular cell-adhesion molecule-1 (ICAM-1) and major histocompatibility complex I (MHC-I) in NSCLC cells. MB-PDT also potentiated CD8+ T cell-mediated cytolysis of lung cancer via granzyme B in lung cancer cells and primary human lung cancer organoids.

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Antimicrobial Photodynamic Therapy with Diode laser and Methylene blue as an adjunct to scaling and root planning: A clinical trial

Cited by 22 publications

References 28 publications

Evaluation of indocyanine-mediated photodynamic therapy cytotoxicity in human osteoblast-like cells: an in vitro study

Evaluation of indocyanine-mediated photodynamic therapy cytotoxicity in human osteoblast-like cells: an in vitro study

First in human measurements of abscess cavity optical properties and methylene blue uptake prior to photodynamic therapy byin vivodiffuse reflectance spectroscopy

Phototherapeutic Induction of Immunogenic Cell Death and CD8+ T Cell-Granzyme B Mediated Cytolysis in Human Lung Cancer Cells and Organoids

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