Antimicrobial photodynamic therapy with phenothiazinium photosensitizers in non-vertebrate model Galleria mellonella infected with Fusarium keratoplasticum and Fusarium moniliforme

Paziani, Mário Henrique; Tonani, Ludmilla; Menezes, Henrique Dantas de; Bachmann, Luciano; Wainwright, Mark; Braga, Gilberto Úbida Leite; Kress, Márcia Regina von Zeska

doi:10.1016/j.pdpdt.2018.12.010

Cited by 27 publications

(35 citation statements)

References 40 publications

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“…For example, when the concentration is greater than 0.0003 mg/mL, MB becomes dimer due to self-aggregation, and its peak excitation wavelength becomes 610 nm. 26 The absorption peak of HMME also changes with the concentration. Due to the change of absorption peak, the absorption rate of the same wavelength laser at di®erent concentrations is di®erent, thus a®ecting the e±ciency of producing singlet oxygen by photosensitizer.…”

Section: Discussionmentioning

confidence: 99%

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Liang

Zou

et al. 2020

J. Innov. Opt. Health Sci.

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The main objective of this study is to evaluate the antibacterial effect of antibacterial photodynamic therapy (aPDT) on Streptococcus mutans (S. mutans) biofilm model in vitro. The selection of photosensitizers is the key step for the efficacy of photodynamic therapy (PDT). However, no studies have been conducted in the oral field to compare the functional characteristics and application effects of PDT mediated by various photosensitizers. In this research, the antibacterial effect of Methylene blue (MB)/650[Formula: see text]nm laser and Hematoporphyrin monomethyl ether (HMME)/532[Formula: see text]nm laser on S. mutans biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two PDT. The yield of lactic acid was analyzed by Colony forming unit (CFU) and spectrophotometry, and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy (CLSM) to evaluate the bactericidal effect on each group. Based on the results of CFU, the bacterial colonies formed by 30.4[Formula: see text]J/cm2 532[Formula: see text]nm MB-aPDT group and 30.4[Formula: see text]J/cm2 532[Formula: see text]nm HMME-aPDT group were significantly less than those in other groups, and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT group. Lactic acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control group. The activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4[Formula: see text]J/cm2 aPDT. Therefore, aPDT suitable for energy measurement can kill S. mutans plaque biofilm, and MB-aPDT is better than HMME-aPDT.

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Section: Discussionmentioning

confidence: 99%

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Liang

Zou

et al. 2020

J. Innov. Opt. Health Sci.

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“…Fusarium is a large and diverse fungal genus with great importance in agriculture, human health, and economy. The species are high mycotoxin producers and pathogens to plants and humans [32,33]. Fusarium spp.…”

Section: Discussionmentioning

confidence: 99%

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

et al. 2019

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The aim of this study was to compare the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), phenotypic and molecular methods for the identification of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo (Brazil) between the years 2001 and 2017. Sequencing of ITS region of ribosomal DNA and elongation factor 1 alpha gene (ET1α) were used as reference method in the analysis of a total of 108 Fusarium spp. clinical strains isolated from human hosts with superficial and systemic infections. Agreement between MALDI-TOF-MS and molecular data was observed for 97 out of 108 clinical isolates (89.8%), whereas five (4.6%) and six (5.5%) clinical isolates were misidentified and were not identified by MALDI-TOF MS, respectively. ITS region sequences and MALDI-TOF MS mass spectra identified and grouped correctly most of Fusarium clinical isolates at species complex level. This investigation highlights the potential of MALDI-TOF MS technique as a fast and cost-efficient alternative for clinical Fusarium identification. However, MALDI-TOF MS requires a more accurate and larger database. This work is the first comprehensive report for Fusarium population, based on phenotypic analyses, proteomic profile by MALDI-TOF and phylogenetic analyses of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo, Brazil.

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“…Cultures were grown in YPD liquid media at 37°C with shaking (200 rpm) for 48 hours. Galleria mellonella virulence protocol was conducted according to Paziani et al (2019), with modification 21 . Larvae in the final instar (6˚) stage (0.2 ± 0.025 g) were selected and incubated in glass petri dishes at 37°C for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

“…This insect is the greater wax moth and is used during the sixth instar of the larval stage to screen for virulence of pathogens. This model affords several advantages, most important of which is its ability to withstand mammalian body temperature, but it also is low cost, is easy to use, provides the potential to conduct large‐scale studies, exhibits easily recorded mortality, has some of the virulence factors required for fungal infections in mammals and complies with global trends regarding bioethics issues 19‐21 …”

Section: Introductionmentioning

confidence: 99%

Molecular typing, in vitro susceptibility and virulence of Cryptococcus neoformans/Cryptococcus gattii species complex clinical isolates from south‐eastern Brazil

Barião

Tonani

Cocio

et al. 2020

Mycoses

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Summary Background Cryptococcus neoformans/ Cryptococcus gattii species complex is composed of encapsulated yeast species that are causative agents of cryptococcosis. The characterisation of pathogenic Cryptococcus species provides useful data for epidemiological studies as well as the clinical diagnosis and treatment of patients. Objectives This study aimed to characterise the epidemiology, antifungal susceptibility and virulence of 72 clinical strains isolated from cryptococcosis cases between 2012 and 2017 in a tertiary reference hospital in south‐eastern Brazil. Methods Species and molecular types were molecularly assessed by PCR and PCR‐restriction fragment length polymorphism (RFLP) of the URA5 gene. Antifungal susceptibility testing was performed according to the CLSI protocols. The virulence was studied in a Galleria mellonella infection model. Results The most frequently isolated strain was C. neoformans molecular type VNI (61/72; 84.7%), although C. neoformans molecular type VNII (3/72; 4.2%) was also isolated. Additionally, C. deuterogattii molecular type VGII (8/72; 11.1%) was present, but most frequently from non‐HIV‐infected patients. Non‐wild‐type phenotype to the antifungals was observed in 26.4% (19/72) of the C. neoformans and C. deuterogattii clinical isolates, and the latter demonstrated higher MIC to fluconazole and itraconazole than C. neoformans clinical isolates. Finally, the virulence of C. neoformans and C. deuterogattii clinical isolates was diverse in G mellonella larvae and uncorrelated with the virulence factors of melanin and capsule. Conclusions The assessment of the spread of cryptococcal species and molecular types as well as the pattern of corresponding antifungal susceptibility and virulence aids in surveil the emergence of resistant strains, ensuring more accurate management of the cryptococcal infection.

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Antimicrobial photodynamic therapy with phenothiazinium photosensitizers in non-vertebrate model Galleria mellonella infected with Fusarium keratoplasticum and Fusarium moniliforme

Cited by 27 publications

References 40 publications

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology

Molecular typing, in vitro susceptibility and virulence of Cryptococcus neoformans/Cryptococcus gattii species complex clinical isolates from south‐eastern Brazil

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