The “green synthesis” method is environmentally benign and cost-effective. This research study adopted an environmentally compatible method for creating Zinc oxide nanoparticles (ZnO NPs) using the capping element Solanum nigrum leaf extract. The X-ray diffraction, Fourier transforms infrared spectroscopy, energy dispersive X-ray analysis, UV-visible spectroscopy, fluorescence spectroscopy, field emission scanning electron microscopy, and antibacterial investigations were employed to evaluate the structural, spectroscopic, and antibacterial characterizations of the green synthesized ZnO NPs. It was shown that the generated ZnO NPs had a typical particle size of 27.75[Formula: see text]nm and a wurtzite hexagonal form. FTIR spectroscopy has confirmed the presence of the functional groups in the generated sample. By using EDX analysis, the ZnO NPs’ fabrication and chemical composition have been determined. The surface texture and aggregation of nanostructure entities were examined using FESEM micrographs. Based on UV-Vis investigations, the bandgap of the ZnO NP was calculated to be 3.82[Formula: see text]eV. The fluorescent spectra revealed a significant emission peak at 542[Formula: see text]nm (green) for an excitation wavelength of 270[Formula: see text]nm. The broad Stokes shift observed in fluorescent spectra is beneficial for practical purposes. The results of the antibacterial test shows that the as-synthesized ZnO NPs can be used in the healthcare and environmental sectors to prevent the growth of harmful bacteria.