Antimicrobial potential of Carica papaya, Ipomoea aquatica, Alpinia galanga and Piper betle against the aquatic microbials

Abstract

Abstract. Saptiani G, Hardi EH, Pebrianto CA, Agustina, Ardhani F. 2016. Antimicrobial potential of Carica papaya, Ipomoea aquatica, Alpinia galanga and Piper betle against the aquatic microbials. Nusantara . This research was aimed to investigate the antimicrobial potential of the leaves extract from Carica papaya, Ipomoea aquatica, Piper betle and Alpinia galanga stem against Aeromonas hydrophyla, Pseudomonas sp., Escherichia coli, and Saprolegnia spp.; the pathogenic microbes in aquatics. These plants were dried and extracted with water and ethanol, separately. The extract were then tested for its inhibition effect to the microbial by in vitro agar disc diffusion (ADD) at the concentration of 100, 200, 400, 600, 800, and 1,000 ppm and minimal inhibitory concentration (MIC) at the concentration of 600, 800, and 1,000 ppm. The result showed that water or ethanol extract f of plants showed potent inhibitor against the aquatic microbial. The ethanol extract of P. betle showed the highest inhibition at 800 ppm, with inhibition zones to A. hydrophyla, and Pseudomonas sp. 12.3 mm, E. coli, and Saprolegnia spp. 12.7 mm. One thousand ppm ethanol extract of P. betle could inhibit the best microbes growth using MIC method to A. hydrophila 189.7 cfu/mL, Pseudomonas sp. 253.0 cfu/mL, Saprolegnia spp.169.3 cfu/mL, and 800 ppm to E. coli 172.7 cfu/mL.

Mangrove plants species from Delta Mahakam, Indonesia with antimicrobial potency

“…The inhibitory zone is formed at 14 hours after incubation, and then extends for up to 48 hours. In general, the inhibit zone does not show any increase after 48 hours (Saptiani et al 2016c).…”

“…12.33 mm, while the water extract can produce inhibition zone to Saprolegnia sp., and E. coli about 12.33 mm, A. hydrophila 12.00 mm, and Pseudomonas sp. 11.67 mm, as well as Carica papaya and Alpina galanga extracts can be used as antimicrobial ingredients (Saptiani et al 2016c). Several scientific studies around the world have found evidence that mangrove leaf extract has great potential against pathogenic microbes (Rastegar and Gozari 2017).…”

“…V. harveyi were cultured on Tryptone Soya Agar (TSA) medium plus 3% NaCl, S. aureus and A. hydrophila were cultured on Tryptone Soya Agar (TSA), all of which were incubated for 24 hours at 33°C, E. coli was cultured on Brilliant Green Bile Lactose Broth (BGBL) media. Fungi, Saprolegnia sp., cultured on Potato Dextrose Agar (PDA) medium incubated at 36ºC for 48 hours (Saptiani et al 2016c).…”

“…After incubation, observations were performed at 24, 36, 48, and 60 hours after incubation. Observations and measurements were made on the diameter of the clear zone formed around the filter paper (Khajure and Rathod 2010; Saptiani et al 2013;Saptiani et al 2016c).…”

Tanaman Bakau Api-api Putih (Avicenia marina) Berpotensi Menghambat Mikrob Patogen dan Melindungi Post Larva Udang Windu (THE POTENTIAL OF AVICENNIA MARINA TO INHIBITS PATHOGEN MICROBES AND PROTECTS THE POST LARVA OF TIGER PRAWN)

“…Beberapa penelitian awal, menunjukkan bahwa ekstrak Avicenia marina mempunyai aktivitas antibakterial terhadap bakteri patogen, seperti Staphylococcus aureus, Escherichia coli, dan Pseudomonas (Dhayanithi et al, 2012). Produk tanaman yang digunakan sebagai obat herbal untuk terapi, harganya relatif murah dan mempunyai potensi sebagai sumber bahan obat-obatan (Saptiani et al, 2016c).…”