Antimicrobial potentiality of Polyalthia longifolia seed oil against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin

Abstract

The antimicrobial potentiality of Polyalthia longifolia seed oil and its fatty acid methyl ester (FAME) extract was evaluated against multi drug resistant strains of bacteria and fungi of clinical origin. A sensitivity test was performed to determine the resistance pattern for marketed antimicrobial agents against the selected microorganisms. The results of antimicrobial assay revealed that P. longifolia seed oil and its FAME extract were effective against all the microorganisms studied. Enterococcus faecalis, Streptococcus mutans and Streptococcus bovis were observed to be the most susceptible strains. Among the tested Gram-negative bacteria, Klebsiella pneumoniae was found to be the most sensitive, while Salmonella typhimurium was the most resistant bacteria. In the case of Gram-positive bacteria, E. faecalis was the most sensitive, while S. aureus was the most resistant strain. The Candida albicans was found to be highly sensitive to the action of FAME extract with the least minimum inhibitory concentration (MIC) of 19.5 µg/ml. The MIC values were lower than MBC/MFC values suggesting that the P. longifolia seed oil and its FAME extract inhibit the growth of test organisms while being bactericidal/fungicidal at higher concentrations. In addition, the physiochemical properties and fatty acid composition of P. longifolia seed oil were also investigated. Thus, it was found that P. longifolia seed oil offers potential antibacterial property against the reference strains and could be a possible source to obtain new and effective herbal medicines to treat infections caused by multi-drug resistant strains of microorganisms from community as well as hospital settings.

Essential Oils of the Leaf and Stem of Polyalthia viridis Craib and Their Biological Activities

“…Regarding the genus Polyalthia, antimicrobial examinations have only been focused on P longifolia. 12,13 The EOs of this plant inhibited the growth of 2 micro-organisms, S aureus ATCC-29213 and C albicans ATCC-10231, with MIC values of 78 μg/mL. 13 This is the first report of antimicrobial activity of a Vietnamese Polyalthia species.…”

“…The oil of Nigerian P longifolia seed showed potential for antioxidative, anti-inflammatory, antiparasitic and cytotoxic activities, 12 whereas the seed oil of this plant collecting from India showed an MBC/MFC value of 39.0 µg/ mL against 2 bacterial species, Streptococcus mutans and Staphylococcus aureus. 13 In the DPPH radical scavenging assay, the bark EO of P suaveolens had an SC 50 value of 0.71 µg/mL, which was better than that of the bark EO of Enantia chlorantha (SC 50 8.0 µg/mL). 10 According to The Plant List, Polyalthia viridis Craib is the accepted name of this species, with a high confidence level.…”

Antimicrobial Activity and Chemical Composition of “Kpètè-Kpètè”: A Starter of Benin Traditional Beer Tchoukoutou

“…The minimum inhibitory concentrations of starter were determined by macrodilution method [24]. Concentrations tested were 10 mg/mL, 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.625 mg/mL, 0.3125 mg/mL, 0.15625 mg/mL, 0.07812 mg/mL, and 0.03906 mg/mL.…”

Phytochemical Analysis and Elemental Contents of Varieties of <i>Polyalthia longifolia</i> (Sonn.) Thwaites

“…pendula 6 . This plant is used in traditional medicine across several Asian and African countries, with Ghana inclusive 7,8 for the management of diseases such as malaria, fever, skin diseases, diabetes, hypertension and helminthiasis 2,4,6,9,10 . P. longifolia aqueous extract has been shown to lower the blood pressure, rate of respiration and glucose level in animal models 9 .…”

“…This plant is used in traditional medicine across several Asian and African countries, with Ghana inclusive 7,8 for the management of diseases such as malaria, fever, skin diseases, diabetes, hypertension and helminthiasis 2,4,6,9,10 . P. longifolia aqueous extract has been shown to lower the blood pressure, rate of respiration and glucose level in animal models 9 . The plant is reported to possess antibacterial, antifungal, antitumor, anti-ulcer and antioxidant properties, cytotoxic function and hypotensive effects 2,10 , hepatoprotective and anti-inflammatory, antimicrobial, hypoglycemic and anti-ulcer activities 4,9,11 .…”