Antimicrobial profile of Salmonella enterica serotype Choleraesuis from free-range swine in Kakamega fish market, western Kenya

Onyango, David Miruka; Ndeda, Violet M.; Wandili, Sarah; Wawire, Sifuna Anthony; Ochieng, P.

doi:10.3855/jidc.3861

Cited by 6 publications

(6 citation statements)

References 62 publications

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“…Moreover, cephalosporin-resistant isolates have frequently been detected in S . Choleraesuis and may therefore pose another threat to public health [ 32 , 41 , 42 ]. However, of the six cephalosporin antibiotics tested in our study, no cephalosporin-resistant isolates were detected.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Molecular Investigation of H2S-Negative Salmonella enterica subsp. enterica serovar Choleraesuis Isolates in China

Xie

Zhu³

et al. 2015

PLoS ONE

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Salmonella enterica subsp. enterica serovar Choleraesuis is a highly invasive pathogen of swine that frequently causes serious outbreaks, in particular in Asia, and can also cause severe invasive disease in humans. In this study, 21 S. Choleraesuis isolates, detected from 21 patients with diarrhea in China between 2010 and 2011, were found to include 19 H2S-negative S. Choleraesuis isolates and two H2S-positive isolates. This is the first report of H2S-negative S. Choleraesuis isolated from humans. The majority of H2S-negative isolates exhibited high resistance to ampicillin, chloramphenicol, gentamicin, tetracycline, ticarcillin, and trimethoprim-sulfamethoxazole, but only six isolates were resistant to norfloxacin. In contrast, all of the isolates were sensitive to cephalosporins. Fifteen isolates were found to be multidrug resistant. In norfloxacin-resistant isolates, we detected mutations in the gyrA and parC genes and identified two new mutations in the parC gene. Pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and clustered regularly interspaced short palindromic repeat (CRISPR) analysis were employed to investigate the genetic relatedness of H2S-negative and H2S-positive S. Choleraesuis isolates. PFGE revealed two groups, with all 19 H2S-negative S. Choleraesuis isolates belonging to Group I and H2S-positive isolates belonging to Group II. By MLST analysis, the H2S-negative isolates were all found to belong to ST68 and H2S-positive isolates belong to ST145. By CRISPR analysis, no significant differences in CRISPR 1 were detected; however, one H2S-negative isolate was found to contain three new spacers in CRISPR 2. All 19 H2S-negative isolates also possessed a frame-shift mutation at position 760 of phsA gene compared with H2S-positive isolates, which may be responsible for the H2S-negative phenotype. Moreover, the 19 H2S-negative isolates have similar PFGE patterns and same mutation site in the phsA gene, these results indicated that these H2S-negative isolates may have been prevalent in China. These findings suggested that surveillance should be increased of H2S-negative S. Choleraesuis in China.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Molecular Investigation of H2S-Negative Salmonella enterica subsp. enterica serovar Choleraesuis Isolates in China

Xie

Zhu³

et al. 2015

PLoS ONE

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“…In addition, Molino et al [43] revealed that S. Choleraesuis strain was resistant to two or more antibiotics. Onyango et al [44] found that S. Choleraesuis isolated from swine feces were sulfamethoxazole resistant. Different findings on antimicrobial susceptibility may be attributed to the genetic variability in these strains in different countries.…”

Section: Discussionmentioning

confidence: 99%

Selection and characterization of bacteriophages specific to Salmonella Choleraesuis in swine

2022

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Background and Aim: Salmonella Choleraesuis is the most common serotype that causes salmonellosis in swine. Recently, the use of bacteriophages as a potential biocontrol strategy has increased. Therefore, this study aimed to isolate and characterize bacteriophages specific to S. Choleraesuis associated with swine infection and to evaluate the efficacy of individual phages and a phage cocktail against S. Choleraesuis strains in simulated intestinal fluid (SIF). Materials and Methods: Three strains of S. Choleraesuis isolated from pig intestines served as host strains for phage isolation. The other 10 Salmonella serovars were also used for the phage host range test. The antibiotic susceptibility of the bacterial strains was investigated. Water samples from natural sources and drain liquid from slaughterhouses were collected for phage isolation. The isolated phages were characterized by determining the efficiency of plating against all Salmonella strains and the stability at a temperature range (4°C–65°C) and at low pH (2.5–4.0) in simulated gastric fluids (SGFs). Furthermore, morphology and genomic restriction analyses were performed for phage classification phages. Finally, S. Choleraesuis reduction in the SIF by the selected individual phages and a phage cocktail was investigated. Results: The antibiotic susceptibility results revealed that most Salmonella strains were sensitive to all tested drugs. Salmonella Choleraesuis KPS615 was multidrug-resistant, showing resistance to three antibiotics. Nine phages were isolated. Most of them could infect four Salmonella strains. Phages vB_SCh-RP5i3B and vB_SCh-RP61i4 showed high efficiency in infecting S. Choleraesuis and Salmonella Rissen. The phages were stable for 1 h at 4°C–45°C. However, their viability decreased when the temperature increased to 65°C. In addition, most phages remained viable at a low pH (pH 2.5–4.0) for 2 h in SGF. The efficiency of phage treatment against S. Choleraesuis in SIF showed that individual phages and a phage cocktail with three phages effectively reduced S. Choleraesuis in SIF. However, the phage cocktails were more effective than the individual phages. Conclusion: These results suggest that the newly isolated phages could be promising biocontrol agents against S. Choleraesuis infection in pigs and could be orally administered. However, further in vivo studies should be conducted.

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“…Genomic DNA of each strain was used as a template in PCR reactions to detect the presence of eight antibiotic resistance genes as previously described. These genes included ampC (Hanson et al, 1999), blaCTX (Moghaddam, Beidokhti, Jamehdar, & Ghahraman, 2014), blaTEM (Bert, Bramger, & Lambert-Zochovsky, 2002), blaZ (Olsen, Christensen, & Aarestrup, 2006), mecA (Duran, Ozer, Duran, Onlen, & Demir, 2012), oxa1 (Onyango, Ndeda, Wandili, Wawire, & Ochieng, 2014), oxa9 (Hanson et al, 1999), and shv (Fang, Ataker, Hedin, & Dornbusch, 2008).…”

Section: Detection Of Antibiotic Resistance Genes In Enterobacterial mentioning

confidence: 99%

Diversity and antibiotic resistance patterns of enterobacteria isolated from seafood in Thailand

Pongsilp

Nimnoi

2018

CyTA - Journal of Food

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Contamination with enterobacteria was detectable in 89% of seafood samples from three central seafood markets in Thailand. The average numbers obtained from the same type of seafood were between 1.3 ± 0.9 and 4.5 ± 1.3 log CFU/g per sample. Eighty-one strains and 16 species were distinguished based on ERIC-PCR patterns and TP-RAPD patterns, respectively. The highest prevalence (90% of strains) was resistant to penicillin G whereas none was resistant to gentamycin. In addition, 63% exhibited multidrug resistance. The 16S rDNA sequences of a representative strain from each species exhibited 99% identity to either one of six genera including Citrobacter, Enterobacter, Klebsiella, Providencia, Serratia, and Yersinia. Three β-lactamase genes including blaTEM, ampC, and shv were detected at the frequencies of 43%, 27%, and 24%, respectively. The representative strains possessing β-lactamase genes exhibited β-lactamase activity ranging from 1.96 ± 0.88 to 11.3 ± 0.37 μmol of hydrolyzed nitrocefin/min/mg protein. Diversidad y patrones de resistencia a los antibióticos en enterobacterias aisladas de mariscos en Tailandia RESUMEN En 80% de las muestras de mariscos tomadas en tres mercados centrales de mariscos de Tailandia fue posible detectar contaminación por enterobacterias. Las mediciones promedio de enterobacterias obtenidas en el mismo tipo de marisco fluctúan entre 1.3 ± 0.9 y 4.5 ± 1.3 log cfu/g por muestra. A partir de patrones ERIC-PCR y TP-RAPD se distinguieron 81 cepas y 16 especies, respectivamente. Un porcentaje más elevado de las cepas (90%) es resistente a la penicilina G y ninguna de ellas es resistente a la gentamicina. Asimismo, 63% de las mismas exhibe resistencia a múltiples drogas. Las secuencias 16S ADNr de una cepa representativa de cada una de las especies exhibieron 99% de identidad con uno de seis géneros, incluyendo Citrobacter, Enterobacter, Klebsiella, Providencia, Serratia y Yersinia. Por otra parte, se detectaron tres genes de β-lactamasa, entre los que se incluyen blaTEM, ampC, y shv, con frecuencias de 43%, 27% y 24%, respectivamente. En las cepas representativas que poseen genes de β-lactamasa la actividad de esta enzimaoscila entre 1.96 ± 0.88 y 11.3 ± 0.37 μmol de proteína de nitrocefina/min/mg hidrolizada.

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Antimicrobial profile of Salmonella enterica serotype Choleraesuis from free-range swine in Kakamega fish market, western Kenya

Cited by 6 publications

References 62 publications

Antimicrobial Resistance and Molecular Investigation of H2S-Negative Salmonella enterica subsp. enterica serovar Choleraesuis Isolates in China

Antimicrobial Resistance and Molecular Investigation of H2S-Negative Salmonella enterica subsp. enterica serovar Choleraesuis Isolates in China

Selection and characterization of bacteriophages specific to Salmonella Choleraesuis in swine

Diversity and antibiotic resistance patterns of enterobacteria isolated from seafood in Thailand

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