Antimicrobial proline-rich peptides from the hemolymph of marine snail Rapana venosa

Dolashka, Pavlina; Moshtanska, Vesela; Borisova, Valika; Dolashki, Aleksander; Stevanović, Stefan; Dimanov, Tzvetan; Voelter, Wolfgang

doi:10.1016/j.peptides.2011.05.001

Cited by 69 publications

(53 citation statements)

References 41 publications

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“…24,25 The specific amino acid-enriched peptides from marine species include the proline-rich, [26][27][28] arginine-rich 29,30 and glycine-rich. 31 Hyastatin, an 11.7 kDa glycine-rich (11.6%, 15 glycines in 129 total residues) peptide from the spider crab hemocytes, 31 possesses activity against Gram-positive and Gram-negative bacteria as well as fungi.…”

Section: Discussionmentioning

confidence: 99%

Identification, synthesis and characterization of a novel antimicrobial peptide HKPLP derived from Hippocampus kuda Bleeker

Sun

Jing

et al. 2012

J Antibiot

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A novel gene encoding 55 amino-acid residues has been identified from the brooding pouch cDNA library of Hippocampus kuda Bleeker. The deduced amino-acid sequence is highly homologous to several pleurocidin-like peptides from the winter flounder and comprises a signal peptide, a pro-peptide and a mature peptide. The glycine-rich mature peptide, designated HKPLP, contains 24 amino-acid residues and has been synthesized by solid-phase peptide synthesis. The purified HKPLP exhibits antimicrobial activity against several Gram-positive and Gram-negative bacterial strains at low concentrations (MIC 1.5-7.5 lM). Thermal stability assay data show good heat stability. CD spectroscopy experiments indicate that the dominant contents are anti-parallel and parallel sheets, which may have b-sheet or b-strand motif. It is inferred that HKPLP participates in the host defense during egg fertilization and embryo development as an antimicrobial peptide in brooding pouch.

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Section: Discussionmentioning

confidence: 99%

Identification, synthesis and characterization of a novel antimicrobial peptide HKPLP derived from Hippocampus kuda Bleeker

Sun

Jing

et al. 2012

J Antibiot

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“…Antimicrobial peptides (AMPs) are a major part of the innate immune defense mechanism and have been extensively studied in the past 20 years. AMPs have broad-spectrum activity against different organisms including Gram-positive and Gram-negative bacteria (Zhu et al 2014; Dolashka et al 2011; Zhuang et al 2015), as well as fungi, viruses, parasites, and some cancer cells (Choi and Lee 2014; Bulmer et al 2010; Riciluca et al 2012; Badani et al 2014; Liu et al 2012; Adade et al 2013; Deng et al 2016). …”

Section: Introductionmentioning

confidence: 99%

Prediction and characterization of a novel hemocyanin-derived antimicrobial peptide from shrimp Litopenaeus vannamei

et al. 2018

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Hemocyanin, the multifunctional glycoprotein in the hemolymph of invertebrates, can generate various antimicrobial peptides (AMPs). Given the rising interest in the use of natural therapeutic agents such as AMPs, alternative and more efficient methods for their generation are being explored. In this work, free online software was first applied to predict the generation of antimicrobial peptides from the large subunit of Litopenaeus vannamei hemocyanin. Twenty potential antimicrobial peptides ranging from 1.5 to 1.9 kDa were predicted, five of which had α-helical structures and were selected for antibacterial activity testing. The results indicated that these five peptides had antibacterial activity against seven different bacteria. Of the five peptides, one peptide, designated L1, had the strongest antibacterial activity against both Gram-negative and Gram-positive bacteria. Moreover, CD and NMR data showed that L1 had both α-helical and β-turns structural composition, and that these structures were essential for L1’s antibacterial activity. Furthermore, SEM analysis revealed that peptide L1 had broad-spectrum activity against both Gram-positive and Gram-negative bacteria, as it could destroy the bacterial cell walls and kill the bacteria. Thus, L1 is a very potent antimicrobial peptide that can be exploited and used in antibacterial therapeutics.Electronic supplementary materialThe online version of this article (10.1007/s00726-018-2575-x) contains supplementary material, which is available to authorized users.

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“…The antibacterial activity of blue crab hemolymph is severely depressed in polluted waters [26] . Thus, callinectin may provide a useful biomarker for health assessment of coastal ecosystems [27] .…”

Section: Callinectinmentioning

confidence: 99%

Hemolymph proteins in marine crustaceans

Fredrick

Ravichandran

2012

Asian Pacific Journal of Tropical Biomedicine

100

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Antimicrobial proline-rich peptides from the hemolymph of marine snail Rapana venosa

Cited by 69 publications

References 41 publications

Identification, synthesis and characterization of a novel antimicrobial peptide HKPLP derived from Hippocampus kuda Bleeker

Identification, synthesis and characterization of a novel antimicrobial peptide HKPLP derived from Hippocampus kuda Bleeker

Prediction and characterization of a novel hemocyanin-derived antimicrobial peptide from shrimp Litopenaeus vannamei

Hemolymph proteins in marine crustaceans

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