Antimicrobial properties of 6-pentyl-α-pyrone produced by endophytic strains of Trichoderma koningii and its effect on aflatoxin B1 production

Ismaiel, Ahmed A.; Ali, Dalia M. I.

doi:10.1515/biolog-2017-0173

Cited by 22 publications

(17 citation statements)

References 40 publications

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“…Isotopic labeling experiments in T. atroviride in the early 1990s suggested the oxidation of linoleic acid by a lipoxygenase as a first step in the biosynthesis of 6-PP, one of T. atroviride ’s main secondary metabolites with antifungal and plant growth promoting properties ( Scarselletti and Faull, 1994 ; Vinale et al, 2008 ; Garnica-Vergara et al, 2016 ; Ismaiel and Ali, 2017 ). In this study, we showed, however, that 6-PP biosynthesis in T. atroviride is not affected by deletion of the single lipoxygenase-encoding gene lox1 encoded in its genome.…”

Section: Discussionmentioning

confidence: 99%

The Lipoxygenase Lox1 Is Involved in Light‐ and Injury-Response, Conidiation, and Volatile Organic Compound Biosynthesis in the Mycoparasitic Fungus Trichoderma atroviride

et al. 2020

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The necrotrophic mycoparasite Trichoderma atroviride is a biological pest control agent frequently applied in agriculture for the protection of plants against fungal phytopathogens. One of the main secondary metabolites produced by this fungus is 6-pentyl-α-pyrone (6-PP). 6-PP is an organic compound with antifungal and plant growth-promoting activities, whose biosynthesis was previously proposed to involve a lipoxygenase (Lox). In this study, we investigated the role of the single lipoxygenase-encoding gene lox1 encoded in the T. atroviride genome by targeted gene deletion. We found that light inhibits 6-PP biosynthesis but lox1 is dispensable for 6-PP production as well as for the ability of T. atroviride to parasitize and antagonize host fungi. However, we found Lox1 to be involved in T. atroviride conidiation in darkness, in injury-response, in the production of several metabolites, including oxylipins and volatile organic compounds, as well as in the induction of systemic resistance against the plant-pathogenic fungus Botrytis cinerea in Arabidopsis thaliana plants. Our findings give novel insights into the roles of a fungal Ile-group lipoxygenase and expand the understanding of a light-dependent role of these enzymes.

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Section: Discussionmentioning

confidence: 99%

The Lipoxygenase Lox1 Is Involved in Light‐ and Injury-Response, Conidiation, and Volatile Organic Compound Biosynthesis in the Mycoparasitic Fungus Trichoderma atroviride

et al. 2020

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Section: Discussionmentioning

confidence: 99%

“…6PP produced by Trichoderma spp. promotes plant growth, shows activity against pathogens, and regulates root architecture by inhibiting primary root growth and inducing lateral root formation (Garnica-Vergara et al, 2016;Ismaiel & Ali, 2017). The UFRAB01 strain of B. amyloliquefaciens used in our study was evaluated on oil palm plants in the laboratory and was found to be beneficial to produce indole acetic acid (IAA), 1-carboxylic acid-1-aminocyclopropane (ACC), and abscisic acid (ABA) (Lima et al, 2020).…”

Section: Benefits Of Soybean Co-inoculationmentioning

confidence: 99%

Co‐inoculation of soybean with Bradyrhizobium spp. and Amazonian strains of Trichoderma asperellum and Bacillus amyloliquefaciens increases the productivity and profitability of the crop

Leite,

Hungria,

Leite

et al. 2023

Crop Science

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Co‐inoculation of soybean with plant growth‐promoting microorganisms has the potential to increase yield, and the Amazon is a biome rich in these beneficial microorganisms. Soybean is the most important grain crop in Brazil and is highly benefited by inoculation with Bradyrhizobium spp. strains, both of which represent exotic symbiotic partners. The objective of this study was to evaluate the effects of soybean co‐inoculation with Amazonian strains of Trichoderma asperellum and Bacillus amyloliquefaciens. Greenhouse and field experiments were conducted during the 2020 and 2021 crop seasons in Brazil, respectively. The experimental design was completely randomized in the greenhouse, and randomized blocks were used in the field, both with 3 × 2 factorial schemes. Standard inoculation with Bradyrhizobium and co‐inoculation with T. asperellum and B. amyloliquefaciens was considered the first factor, and the second factor was represented by phosphate fertilization (0 and 100 kg ha−1 of P2O5). The root system volumes were 25% and 17% higher than those of the standard inoculation with T. asperellum and B. amyloliquefaciens, respectively. In 2020, co‐inoculation with T. asperellum increased yield by 15%, plant height by 27%, and net profit by up to 121 USD compared with standard inoculation. In 2021, co‐inoculation with T. asperellum and B. amyloliquefaciens resulted in increases in several growth parameters compared to those with standard inoculation, including 7% and 13% greater yields, and 159 USD and 309 USD increases in net profit, respectively. Our results indicate the possibility of expanding soybean co‐inoculation with local strains of Bacillus and Trichoderma, with benefits for productivity and profitability.

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“…They were morphologically identified and deposited in the Assiut University Mycological Center (AUMC, http://www.aun . edu.eg/aumc/aumc.htm) with accession numbers: AUMC11519, AUMC11520, AUMC11521, and AUMC11522, respectively (Ismaiel and Ali 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of ochratoxin A by endophytic Trichoderma koningii strains

Ismaiel

Mohamed

El-Sayed

2022

World J Microbiol Biotechnol

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Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus spp. and Penicillium spp. that causes a threat to food safety and human health. Fungal biodegradation might be a promising strategy for reducing the OTA contamination in the future. In this study, the ability of Trichoderma koningii strains to degrade OTA produced by Aspergillus niger T2 (MW513392.1) isolated from tomato seeds was investigated. Among T. koningii strains tested, three strains; AUMC11519, AUMC11520 and AUMC11521 completely eliminated OTA from the culture medium, while AUMC11522 strain eliminated only 41.82% of OTA. OTα-amide, 3-phenylpropionic acid, OTα and phenylalanine were assayed as degradation products by FTIR analysis and LC–MS/MS spectra. Carboxypeptidase A (CPA) was found responsible for OTA degradation when a metal ion chelator, EDTA, was added to cell free supernatants of the three effective strains. OTA detoxification by T. koningii could present new prospective strategies for a possible application in food commodities intoxicated with ochratoxin.

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Antimicrobial properties of 6-pentyl-α-pyrone produced by endophytic strains of Trichoderma koningii and its effect on aflatoxin B1 production

Abstract: A total of 60 endophytic fungal strains were isolated and screened for their producing-ability of 6-pentyl-

Cited by 22 publications

References 40 publications

The Lipoxygenase Lox1 Is Involved in Light‐ and Injury-Response, Conidiation, and Volatile Organic Compound Biosynthesis in the Mycoparasitic Fungus Trichoderma atroviride

The Lipoxygenase Lox1 Is Involved in Light‐ and Injury-Response, Conidiation, and Volatile Organic Compound Biosynthesis in the Mycoparasitic Fungus Trichoderma atroviride

Co‐inoculation of soybean with Bradyrhizobium spp. and Amazonian strains of Trichoderma asperellum and Bacillus amyloliquefaciens increases the productivity and profitability of the crop

Biodegradation of ochratoxin A by endophytic Trichoderma koningii strains

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