Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria

Domingues, Marco M.; Silva, Patrícia M.; Franquelim, Henri G.; Carvalho, Filomena A.; Castanho, Miguel A. R. B.; Santos, Nuno C.

doi:10.1016/j.nano.2013.11.002

Cited by 86 publications

(61 citation statements)

References 48 publications

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“…The resulting increased local pH activates autolysin, which would weaken the cell wall by breaking down glycosidic bonds and peptide crosslinks. A similar argument has been made recently for triggering cell lysis in Staphlococcus aureus by the antimicrobial peptide rBPI21 [40], although the effect was not associated with peptides anchored on a surface.…”

Section: Resultssupporting

confidence: 75%

Antimicrobial GL13K Peptide Coatings Killed and Ruptured the Wall of Streptococcus gordonii and Prevented Formation and Growth of Biofilms

Chen

Hirt

et al. 2014

PLoS ONE

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Infection is one of the most prevalent causes for dental implant failure. We have developed a novel antimicrobial peptide coating on titanium by immobilizing the antimicrobial peptide GL13K. GL13K was developed from the human salivary protein BPIFA2. The peptide exhibited MIC of 8 µg/ml against planktonic Pseudonomas aeruginosa and their biofilms were reduced by three orders of magnitude with 100 µg/ml GL13K. This peptide concentration also killed 100% of Streptococcus gordonii. At 1 mg/ml, GL13K caused less than 10% lysis of human red blood cells, suggesting low toxicity to mammalian cells. Our GL13K coating has also previously showed bactericidal effect and inhibition of biofilm growth against peri-implantitis related pathogens, such as Porphyromonas gingivalis. The GL13K coating was cytocompatible with human fibroblasts and osteoblasts. However, the bioactivity of antimicrobial coatings has been commonly tested under (quasi)static culture conditions that are far from simulating conditions for biofilm formation and growth in the oral cavity. Oral salivary flow over a coating is persistent, applies continuous shear forces, and supplies sustained nutrition to bacteria. This accelerates bacteria metabolism and biofilm growth. In this work, the antimicrobial effect of the coating was tested against Streptococcus gordonii, a primary colonizer that provides attachment for the biofilm accretion by P. gingivalis, using a drip-flow biofilm bioreactor with media flow rates simulating salivary flow. The GL13K peptide coatings killed bacteria and prevented formation and growth of S. gordonii biofilms in the drip-flow bioreactor and under regular mild-agitation conditions. Surprisingly the interaction of the bacteria with the GL13K peptide coatings ruptured the cell wall at their septum or polar areas leaving empty shell-like structures or exposed protoplasts. The cell wall rupture was not detected under regular culture conditions, suggesting that cell wall rupture induced by GL13K peptides also requires media flow and possible attendant biological sequelae of the conditions in the bioreactor.

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Section: Resultssupporting

confidence: 75%

Antimicrobial GL13K Peptide Coatings Killed and Ruptured the Wall of Streptococcus gordonii and Prevented Formation and Growth of Biofilms

Chen

Hirt

et al. 2014

PLoS ONE

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“…albicans planktonic cells presented a significant increase in surface roughness after treatment with AMPH B, FCZ or Ps d1. Upon surface analysis of AFM height images (Tyagi and Malik, 2010a,b; Domingues et al, 2013; Franquelim et al, 2013), it was possible to quantify the increase in membrane roughness that had been noticed on the error signal images. The fact that Δ gcs cells were less affected by these substances may be due to the fact that they are already rougher (Figure 2G) than CI and WT strains, even before treatment with AMPH B, FCZ or Ps d1.…”

Section: Discussionmentioning

confidence: 99%

Psd1 Effects on Candida albicans Planktonic Cells and Biofilms

Gonçalves

Silva

Felício

et al. 2017

Front. Cell. Infect. Microbiol.

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Candida albicans is an important human pathogen, causing opportunistic infections. The adhesion of planktonic cells to a substrate is the first step for biofilm development. The antimicrobial peptide (AMP) Psd1 is a defensin isolated from Pisum sativum seeds. We tested the effects of this AMP on C. albicans biofilms and planktonic cells, comparing its activity with amphotericin B and fluconazole. Three C. albicans variants were studied, one of them a mutant deficient in glucosylceramide synthase, conferring resistance to Psd1 antifungal action. Atomic force microscopy (AFM) was used to assess morphological and biomechanical changes on fungal cells. Surface alterations, with membrane disruption and leakage of cellular contents, were observed. Cytometry assays and confocal microscopy imaging showed that Psd1 causes cell death, in a time and concentration-dependent manner. These results demonstrate Psd1 pleiotropic action against a relevant fungal human pathogen, suggesting its use as natural antimycotic agent.

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“…Dilutions in 10 mmol/L NaCl aqueous solutions were made for the nanocapsule analysis, as described earlier. Results were expressed as a mean of 3 independent measurements, 41 and bacterial concentration was fixed at 5×10 5 CFU/mL to reproduce the first well of the serial microdilution testing condition.…”

Section: Mic In Yeastmentioning

confidence: 99%

“…55 The zeta potential determined for the P. aeruginosa inoculum was −4.18±1.6 mV (pure inoculum). Negative potential enhanced the interaction with positive ions, 41 such as cationic-coated nanocapsules. The zeta potential of P. aeruginosa inoculum shifted to +3.19±0.27 mV after contact with NC AC (Figure 3).…”

Section: Zeta Potential Of P Aeruginosa Before and After Contact Witmentioning

confidence: 99%

“…The slight negative zeta potential could be a barrier to prevent NC BC interaction with the bacterial membrane, which also has negative zeta potential. 41 On the other hand, NC AC promoted an increase of 1.6-folds and 1.8-folds in comparison to T P80 and a P80 , respectively. In a study performed with positive PCL nanocapsules containing chlorhexidine (free base), it was suggested that the cationic nanoparticles were able to interact with bacteria, due to their opposite charges, diffusing the drug from the core of the nanocapsules to the bacterial cell membrane.…”

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confidence: 91%

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Triclosan resistance reversion by encapsulation in chitosan-coated-nanocapsule containing α-bisabolol as core: development of wound dressing

Marchi¹,

Jornada²,

Silva³

et al. 2017

IJN

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The use of nanoparticles may be particularly advantageous in treating bacterial infections due to their multiple simultaneous mechanisms of action. Nanoencapsulation is particularly useful for lipophilic drugs. In this scenario, triclosan is considered a good candidate due to its lipophilicity, broad-spectrum activity, and safety. In the present study, we have developed and characterized an antimicrobial suspension of triclosan and α-bisabolol against pathogenic strains that are resistant ( Pseudomonas aeruginosa ) and susceptible ( Escherichia coli , Staphylococcus aureus , and Candida albicans ) to triclosan. We also aimed to determine the minimum inhibitory concentration, using serial microdilution adapted from a CLSI methodology (Clinical and Laboratory Standards Institute). Challenge test was used to confirm the antimicrobial effectiveness of the nanocapsule formulation, as well as after its incorporation into a commercial wound dressing (Veloderm ® ). The zeta potential of P. aeruginosa before and after contact with cationic nanocapsules and the ratio between the number of nanocapsules per colony forming unit (CFU) were determined to evaluate a possible interaction between nanocapsules and bacteria. The results showed that nanoencapsulation has improved the antimicrobial activity when tested with two different methodologies. The number of nanocapsules per CFU was high even in great dilutions and the zeta potential was reverted after being in contact with the cationic nanocapsules. The nanocapsules were able to improve the activity of triclosan, even when tested within 28 days and when dried in the wound dressing.

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Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria

Cited by 86 publications

References 48 publications

Antimicrobial GL13K Peptide Coatings Killed and Ruptured the Wall of Streptococcus gordonii and Prevented Formation and Growth of Biofilms

Antimicrobial GL13K Peptide Coatings Killed and Ruptured the Wall of Streptococcus gordonii and Prevented Formation and Growth of Biofilms

Psd1 Effects on Candida albicans Planktonic Cells and Biofilms

Triclosan resistance reversion by encapsulation in chitosan-coated-nanocapsule containing α-bisabolol as core: development of wound dressing

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Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria

Cited by 86 publications

References 48 publications

Antimicrobial GL13K Peptide Coatings Killed and Ruptured the Wall of Streptococcus gordonii and Prevented Formation and Growth of Biofilms

Antimicrobial GL13K Peptide Coatings Killed and Ruptured the Wall of Streptococcus gordonii and Prevented Formation and Growth of Biofilms

Psd1 Effects on Candida albicans Planktonic Cells and Biofilms

Triclosan resistance reversion by encapsulation in chitosan-coated-nanocapsule containing &alpha;-bisabolol as core: development of wound dressing

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Product

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Triclosan resistance reversion by encapsulation in chitosan-coated-nanocapsule containing α-bisabolol as core: development of wound dressing