Antimicrobial resistance and genetic characterization of fluoroquinolone resistance of Pseudomonas aeruginosa isolated from canine infections

Rubin, Joseph E.; Walker, Robert D.; Blickenstaff, Karen; Bodeis-Jones, Sonya; Zhao, Shaohua

doi:10.1016/j.vetmic.2008.02.018

Cited by 99 publications

(135 citation statements)

References 23 publications

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“…On the other hand, the resistance to marbofloxacin found in this study is lower than the resistance rates of between 25% and 49% recorded in other studies (McKAY et al, 2007;RUBIN et al, 2008;MÜLLER and HOM, 2009). The main reason for this is probably the fact that marbofloxacin is registered in Croatia only for topical treatment of otitis externa.…”

Section: Discussioncontrasting

confidence: 81%

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

2017

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________________________________________________________________________________________ PINTARIĆ, S., K. MATANOVIĆ, B. ŠEOL MARTINEC:Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs -comparing disk diffusion and microdilution methods. Vet. arhiv 87, 291-300, 2017. ABSTRACTPseudomonas aeruginosa has been determined to be the distinct cause of a number of different infections in both humans and animals. Apart from its high intrinsic resistance, which makes it difficult to treat, it also has a great capacity to acquire further resistance mechanisms. These mechanisms can be present simultaneously in one cell, conferring a multiresistant phenotype. Taking into account that the known risk factor for selection of resistant strains is excessive and inappropriate drug use, especially fluoroquinolones, the aim of this study was to determine the susceptibility of P. aeruginosa isolates to these antibacterial agents. The susceptibility of 90 canine isolates was determined by disk diffusion susceptibility test and the broth microdilution method. Both methods showed that isolates were significantly more sensitive to ciprofloxacin than to marbofloxacin or enrofloxacin, and more sensitive to marbofloxacin than to enrofloxacin. The results of microdilution and disk diffusion testing were in 98.9% agreement for ciprofloxacin and in 91.1% agreement for marbofloxacin, with no statistically significant difference between the two methods (P>0.05) for these two antibiotics. For enrofloxacin, the results of microdilution and disk diffusion testing were in 76.6% agreement, with a statistically significant disagreement between the two methods (P<0.05). In 16 cases, the disk diffusion test overestimated the number of strains susceptible to enrofloxacin compared with the microdilution test. These results suggest that for P. aeruginosa infections in dogs, therapy using enrofloxacin, based on the results of disk diffusion testing, may lead to ineffective treatment and could contribute to selection of resistant and multiresistant strains.

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Section: Discussioncontrasting

confidence: 81%

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

2017

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“…MICs of the following 16 antimicrobials, amikacin (AMI), ampicillin (AMP), amoxicillin/clavulanic acid (AUG), cefoxitin (FOX), ceftiofur (XNL), ceftriaxone (AXO), cephalothin (CEP), chloramphenicol (CHL), ciprofloxacin (CIP), gentamicin (GEN), kanamycin (KAN), nalidixic acid (NAL), streptomycin (STR), sulfamethoxazole (SMX), tetracycline (TET) and trimethoprim/sulfamethoxazole (SXT), and 8 fluoroquinolones, ciprofloxacin (CIP), danofloxacin (DANO), difloxacin (DIF), enrofloxacin (ENRO), gatifloxacin (GAT), levofloxacin (LEVO), marbofloxacin (MARB) and orbifloxacin (ORB) were determined using the Sensititre susceptibility system according to the manufacturer's instructions (Trek Diagnostic Systems Inc., Independence, OH, USA). For those drugs that lacked specific interpretive criteria, CLSI breakpoints for Enterobacteriaceae were used [43]. E. coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were used as controls for antimicrobial susceptibility testing.…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Disinfectant and Antimicrobial Susceptibility Profiles of Salmonella Strains from Feedlot Water-Sprinkled Cattle: Hides and Feces

Beier¹,

Callaway²,

Andrews³

et al. 2017

J Food Chem Nanotechnol

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“…However, panaminoglycoside resistance e.g., gentamicin, spectinomycin, streptomycin and amikacin has been increasingly reported in the P. aeruginosa dog and cat isolates [18,19,23]. Such resistance has been clarified to be predominantly due to a poorly understood mechanism namely "impermeability resistance" as a consequence of diminished drug uptake and/or accumulation [21,31].…”

mentioning

confidence: 99%

Contribution of the MexXY Multidrug Efflux Pump and Other Chromosomal Mechanisms on Aminoglycoside Resistance in <i>Pseudomonas aeruginosa</i> Isolates from Canine and Feline Infections

Poonsuk

Chuanchuen

2012

J. Vet. Med. Sci.

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ABSTRACT. As study of multidrug efflux pumps is a crucial step for development of efflux pump inhibitors for treatment of Pseudomonas aeruginosa infection, the objective of this study was to examine the contribution of the MexXY multidrug efflux systems and other chromosomal mechanisms in aminoglycoside (AMG) resistance in P. aeruginosa isolated from dogs and cats. Thirteen Pseudomonas aeruginosa isolates from canine and feline infections were examined for contribution of the MexXY multidrug efflux pump and four other chromosomally-encoded genes including PA5471, galU, nuoG and rplY to AMG resistance. All the isolates were resistant to multiple AMGs and expressed mexXY. Deletion of mexXY caused 2-to 16-fold reduction in AMG MICs. Overproduction of MexXY did not fully account for the observed AMG resistance. No good correlations were detected between MexXY transcription level and AMG MICs. While no mutations were found in mexZ, PA5471 expression varied and its impact on MexXY expression and AMG resistance is diverse. No mutations were found in galU. Only two isolates carried a single base change G-367-T in rplY. Complete transcription of nuoG was detected in all the isolates. In conclusion, the MexXY multidrug efflux pump plays a role in AMG resistance in the dog and cat P. aeruginosa isolates, while disruption of nuoG, rplY and galU did not have a significant impact. These results indicate the existence of uncharacterized AMG-resistance mechanisms. Pseudomonas aeruginosa is infamously known as a common cause of chronic and recurrent infections in both humans and animals, of which the most-notably diseases in dogs and cats include otitis externa/media, urinary tract infection and pyoderma [9,10,20]. Treatment of P. aeruginosa infection is challenging, because the pathogen intrinsically exhibits and efficiently develops high resistance to several antimicrobials structurally and functionally unrelated, leading to multidrug resistance [1,24].As many antipseudomonal drugs are available for therapeutic use in dogs and cats, aminoglycosides (AMGs) are considered a vital component of antipseudomonal chemotherapy [22] due to their efficacy, safety and reasonable price. However, panaminoglycoside resistance e.g., gentamicin, spectinomycin, streptomycin and amikacin has been increasingly reported in the P. aeruginosa dog and cat isolates [18,19,23]. Such resistance has been clarified to be predominantly due to a poorly understood mechanism namely "impermeability resistance" as a consequence of diminished drug uptake and/or accumulation [21,31]. The MexXY efflux system, a multidrug efflux pump in the resistance-nodulation-cell-division (RND) family, is involved in the reduced level of AMG accumulation implicated in both impermeability-type and adaptive-type AMG resistance [12,21,22]. The MexXY efflux pump is encoded by the mexXY operon and evidently serves as the major AMG-resistance mediating system in P. aeruginosa clinical isolates. This system additionally confers resistance to nonaminoglycosides, including tetracycline...

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Antimicrobial resistance and genetic characterization of fluoroquinolone resistance of Pseudomonas aeruginosa isolated from canine infections

Cited by 99 publications

References 23 publications

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

Disinfectant and Antimicrobial Susceptibility Profiles of Salmonella Strains from Feedlot Water-Sprinkled Cattle: Hides and Feces

Contribution of the MexXY Multidrug Efflux Pump and Other Chromosomal Mechanisms on Aminoglycoside Resistance in <i>Pseudomonas aeruginosa</i> Isolates from Canine and Feline Infections

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