Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States

Srednik, Mariela E.; Lantz, Kristina; Hicks, Jessica A.; Morningstar‐Shaw, Brenda; Mackie, Tonya A.; Schlater, Linda K.

doi:10.1371/journal.pone.0249617

Cited by 19 publications

(21 citation statements)

References 30 publications

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“…Dublin in France, which was not previously demonstrated. As previously shown ( 54 , 58 ), S . Dublin is a highly clonal serotype and harbours a highly conserved genome ( 13 , 53 , 54 , 59 ), which is found here with a low number of intra and inter-cluster SNPs, and from the few numbers of SNPs excluded from recombination events.…”

Section: Discussionsupporting

confidence: 84%

A retrospective and regional approach assessing the genomic diversity ofSalmonellaDublin

Violante

Podeur

Michel

et al. 2022

NAR Genomics and Bioinformatics

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From a historically rare serotype, Salmonella enterica subsp. enterica Dublin slowly became one of the most prevalent Salmonella in cattle and raw milk cheese in some regions of France. We present a retrospective genomic analysis of 480 S. Dublin isolates to address the context, evolutionary dynamics, local diversity and the genesis processes of regional S. Dublin outbreaks events between 2015 and 2017. Samples were clustered and assessed for correlation against metadata including isolation date, isolation matrices, geographical origin and epidemiological hypotheses. Significant findings can be drawn from this work. We found that the geographical distance was a major factor explaining genetic groups in the early stages of the cheese production processes (animals, farms) while down-the-line transformation steps were more likely to host genomic diversity. This supports the hypothesis of a generalised local persistence of strains from animal to finished products, with occasional migration. We also observed that the bacterial surveillance is representative of diversity, while targeted investigations without genomics evidence often included unrelated isolates. Combining both approaches in phylogeography methods allows a better representation of the dynamics, of outbreaks.

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Section: Discussionsupporting

confidence: 84%

A retrospective and regional approach assessing the genomic diversity ofSalmonellaDublin

Violante

Podeur

Michel

et al. 2022

NAR Genomics and Bioinformatics

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“…Furthermore, recent data revealed that the proportion of resistance in S . Dublin isolates in the United States is higher than in other Salmonella serotypes [ 18 , 19 , 46 ]. Furthermore, S. Dublin is one of the serotypes showing the highest multidrug resistance [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Investigation of Salmonella Dublin Considering Mountain Pastures as Reservoirs in Southern Bavaria, Germany

Klose¹,

Scuda²,

Ziegler³

et al. 2022

Microorganisms

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Worldwide, Salmonella Dublin (S. Dublin) is responsible for clinical disease in cattle and also in humans. In Southern Bavaria, Germany, the serovar was identified as a causative agent for 54 animal disease outbreaks in herds between 2017 and 2021. Most of these emerged from cattle herds (n = 50). Two occurred in pig farms and two in bovine herds other than cattle. Genomic analysis of 88 S. Dublin strains isolated during these animal disease outbreaks revealed 7 clusters with 3 different MLST-based sequence types and 16 subordinate cgMLST-based complex types. Antimicrobial susceptibility investigation revealed one resistant and three intermediate strains. Furthermore, only a few genes coding for bacterial virulence were found among the isolates. Genome analysis enables pathogen identification and antimicrobial susceptibility, serotyping, phylogeny, and follow-up traceback analysis. Mountain pastures turned out to be the most likely locations for transmission between cattle of different herd origins, as indicated by epidemiological data and genomic traceback analyses. In this context, S. Dublin shedding was also detected in asymptomatic herding dogs. Due to the high prevalence of S. Dublin in Upper Bavaria over the years, we suggest referring to this administrative region as “endemic”. Consequently, cattle should be screened for salmonellosis before and after mountain pasturing.

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“…In contrast, S. Dublin strains from both cattle and human beings coming from non-European countries such as the United States, Canada or China revealed a comprehensive antimicrobial resistance pattern [ 2 , 30 , 31 , 32 , 33 ]. The acquisition of plasmids that carry resistance genes such as IncA/C2, ColRNAI, or incompatibility (Inc) groups of plasmids have been identified in S. Dublin MDR isolates from cattle in the United States [ 34 ]. It is suggested that the difference between the European and American isolates is the carriage of the IncA/C2 plasmid [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is suggested that the difference between the European and American isolates is the carriage of the IncA/C2 plasmid [ 31 ]. Other genetic mechanisms identified include point mutations in resistance genes encoded in the chromosome ( gyr A for fluoroquinolones and quinolones) and multidrug efflux pumps as the ones encoded by the mdfA gene [ 34 ]. Plasmid replicons associated with virulence such as IncFII(S) and IncX1 were the most predominant detected among the strains.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Analysis on the Occurrence of Salmonella enterica Subspecies enterica Serovar Dublin in the German Federal State Schleswig-Holstein Using Whole-Genome Sequencing

2023

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The cattle-adapted serovar Salmonella Dublin (S. Dublin) causes enteritis and systemic diseases in animals. In the German federal state Schleswig-Holstein, S. Dublin is the most important serovar in cattle indicating an endemic character of the infection. To gain information on dissemination and routes of infection, whole-genome sequencing (WGS) was used to explore the genetic traits of 78 S. Dublin strains collected over a period of six years. The phylogeny was analysed using core-genome single nucleotide polymorphisms (cgSNPs). Genomic clusters at 100, 15 and 1 cgSNPs were selected for molecular analysis. Important specific virulence determinants were detected in all strains but multidrug resistance in S. Dublin organisms was not found. Using 15 cgSNPs epidemiological links between herds were identified, clusters at 1 cgSNPs provided clear evidence on both persistence of S. Dublin at single farms in consecutive years and transmission of the organisms between herds in different distances. A possible risk factor for the repeated occurrence of S. Dublin in certain districts of Schleswig-Holstein might be the spreading of manure on pastures and grassland. Effective control of S. Dublin requires farm-specific analysis of the management supplemented by WGS of outbreak causing S. Dublin strains to clearly identify routes of infection.

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Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States

Cited by 19 publications

References 30 publications

A retrospective and regional approach assessing the genomic diversity ofSalmonellaDublin

A retrospective and regional approach assessing the genomic diversity ofSalmonellaDublin

Whole-Genome Investigation of Salmonella Dublin Considering Mountain Pastures as Reservoirs in Southern Bavaria, Germany

Epidemiological Analysis on the Occurrence of Salmonella enterica Subspecies enterica Serovar Dublin in the German Federal State Schleswig-Holstein Using Whole-Genome Sequencing

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