Antimicrobial resistance and integrons of commensal<i>Escherichia coli</i>strains from healthy humans in China

Li, Bin; Zhao, Zhichang; Wang, Meihua; Huang, Xing; Pan, Yu; Cao, Yong

doi:10.1179/1973947813y.0000000113

Cited by 22 publications

(22 citation statements)

References 14 publications

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“…Similar to other investigations, different antibiotic resistance patterns were observed in UPEC compared with commensal isolates [ 31 , 32 ]. We found that the resistance rate of commensal isolates to third-generation cephalosporins and amikacin was higher than was observed in reports from other countries [ 31 – 33 ]. The observed resistance in fecal E coli may be because of the extensive and long-term use of antibiotics [ 21 , 31 , 33 ].…”

Section: Discussionsupporting

confidence: 88%

“…We found that the resistance rate of commensal isolates to third-generation cephalosporins and amikacin was higher than was observed in reports from other countries [ 31 – 33 ]. The observed resistance in fecal E coli may be because of the extensive and long-term use of antibiotics [ 21 , 31 , 33 ]. Furthermore, the considerable number of MDR isolates among the UPEC found in Iran and other countries [ 28 , 34 , 35 ] may be the result of widespread use and misuse of antibiotics in hospitals and in the community [ 36 ].…”

Section: Discussioncontrasting

confidence: 71%

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Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Karam

Habibi²,

Bouzari³

2018

Osong Public Health Res Perspect

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ObjectivesUropathogenic Escherichia coli (UPEC) are the major cause of urinary tract infections (UTIs). Here, we determined whether sensitivity to antibiotics was related to the prevalence of iron scavenging genes, or to biofilm and hemolysis formation.MethodsA total of 110 UPEC and 30 E coli isolates were collected from the urine of UTI patients and feces of healthy individuals without UTI, respectively. The presence of iron receptor genes and phenotypic properties were evaluated by polymerase chain reaction and phenotypic methods, respectively. Susceptibility to routine antibiotics was evaluated using the disc diffusion method.ResultsThe prevalence of iron scavenging genes ranged from 21.8% (ireA) to 84.5% (chuA) in the UPEC. Resistance to ceftazidime and cefotaxime was significantly correlated with the presence of fyuA and iutA iron genes. Biofilm production was significantly associated with the prevalence of fyuA and hma iron genes. A higher degree of antibiotic resistance was exhibited by isolates that produced biofilms than by their non-biofilm producing counterparts.ConclusionOur study clearly indicates that biofilm production is associated with antibiotic resistance, and that iron receptors and hemolysin production also contribute to reduced antibiotic sensitivity. These results further our understanding of the role that these virulence factors play during UPEC pathogenesis, which in turn may be valuable for the development of novel treatment strategies against UTIs.

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Section: Discussionsupporting

confidence: 88%

Section: Discussioncontrasting

confidence: 71%

Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Karam

Habibi²,

Bouzari³

2018

Osong Public Health Res Perspect

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“…e results showed that the prevalence of integrons was low, and some were located on transferable plasmids. e prevalence of the integron (26.5%) in this study was similar to that in our previous report (26.7%) [18]; however, it is lower than that found in clinical Enterobacteriaceae isolates in China (59.9%) [19]. e result suggested that the prevalence of integron was lower in E. coli ST131 as compared with other clinical isolates.…”

Section: Discussionsupporting

confidence: 86%

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Huang

Lan

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background. Escherichia coli sequence type 131 (ST131) is an important multidrug-resistant extraintestinal pathogen, which can cause many kinds of infections. Integrons may play a crucial role in the dissemination of antibiotic resistance genes. e purpose of this study was to characterize the prevelance of integrons among E. coli ST131 strains in China. Methods. Eighty-three E. coli ST131 isolates were used in this study. e antibiotic susceptibility test was performed by the disk diffusion method. e presence and characterization of class 1, 2, and 3 integrons, as well as promotor of gene cassettes and other antimicrobial resistance genes, were detected by PCR and DNA sequencing. Transfer of integrons was carried out using a broth culture mating method. Clonal relatedness of E. coli ST131 isolates was analyzed by PFGE. Results. Overall, 26.5% (22/83) of the E. coli ST131 isolates carried class 1 integrons. Class 2 and 3 integrons were not found in this study. Two types of gene cassette arrays were demonstrated in this study and were as follows: dfrA17-aadA5 and aac(6′)-Ib-cr-cmlA5. Only one type of Pc promoter variant was detected among 22 integron-positive isolates (PcW). In vivo transfer of integron was successful for 9 of integron-positive E. coli ST131 isolates harboring resistance gene cassettes. Results of PFGE demonstrated that the integron-positive E. coli ST131 isolates were grouped into 12 different PFGE clusters. Conclusions. Our study showed a low prevalence of integrons was detected in E. coli ST131. Continued surveillance of this mobile genetic element should be performed to study the evolution of antibiotic resistance among E. coli ST131.

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“…This suggests that the dissemination of integrons was not due to clonal spread, but to horizontal gene transfer of plasmids or transposons, emphasizing the important role of integrons in the spread of antimicrobial resistance genes. The prevalence of integrons (19.4%) in E. coli isolates from healthy humans was higher than that reported in an earlier study from Korea [11], but lower than that in other countries [19, 30]. …”

Section: Discussioncontrasting

confidence: 70%

“…Integrons are known to play an important role in the horizontal transfer of antimicrobial resistance genes by conjugative plasmids and transposons, and closely associated with the development of MDR in enterobacteria [22, 23]. Class I is the predominant class of integron detected in many countries including Korea [11, 19, 26]. Likewise, all integron positive E. coli isolates in this study were found to be multi-drug resistant, and the prevalent type was class I. PFGE analysis revealed that all isolates carrying integrons had distinct PFGE types (Fig.…”

Section: Discussionmentioning

confidence: 99%

Probable secondary transmission of antimicrobial-resistant <i>Escherichia coli</i> between people living with and without pets

Chung

Park

et al. 2017

The Journal of Veterinary Medical Science

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Companion animals are considered as one of the reservoirs of antimicrobial-resistant (AR) bacteria that can be cross-transmitted to humans. However, limited information is available on the possibility of AR bacteria originating from companion animals being transmitted secondarily from owners to non-owners sharing the same space. To address this issue, the present study investigated clonal relatedness among AR E. coli isolated from dog owners and non-owners in the same college classroom or household. Anal samples (n=48) were obtained from 14 owners and 34 non-owners; 31 E. coli isolates were collected (nine from owners and 22 from non-owners). Of 31 E. coli, 20 isolates (64.5%) were resistant to at least one antimicrobial, and 16 isolates (51.6%) were determined as multi-drug resistant E. coli. Six isolates (19.4%) harbored integrase genes (five harbored class I integrase gene and one harbored class 2 integrase gene, respectively). Pulsed-field gel electrophoretic analysis identified three different E. coli clonal sets among isolates, indicating that cross-transmission of AR E. coli can easily occur between owners and non-owners. The findings emphasize a potential risk of spread of AR bacteria originating from pets within human communities, once they are transferred to humans. Further studies are needed to evaluate the exact risk and identify the risk factors of secondarily transmission by investigating larger numbers of isolates from pets, their owners and non-owners in a community.

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Antimicrobial resistance and integrons of commensalEscherichia colistrains from healthy humans in China

Cited by 22 publications

References 14 publications

Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Probable secondary transmission of antimicrobial-resistant <i>Escherichia coli</i> between people living with and without pets

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Antimicrobial resistance and integrons of commensalEscherichia colistrains from healthy humans in China

Cited by 22 publications

References 14 publications

Relationships between Virulence Factors and Antimicrobial Resistance among &lt;italic&gt;Escherichia coli&lt;/italic&gt; Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Relationships between Virulence Factors and Antimicrobial Resistance among &lt;italic&gt;Escherichia coli&lt;/italic&gt; Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Probable secondary transmission of antimicrobial-resistant <i>Escherichia coli</i> between people living with and without pets

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Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran

Relationships between Virulence Factors and Antimicrobial Resistance among <italic>Escherichia coli</italic> Isolated from Urinary Tract Infections and Commensal Isolates in Tehran, Iran