Antimicrobial Resistance and Molecular Characteristics of Methicillin-Resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt; Isolates from Child Patients of High-Risk Wards in Shenzhen, China

Yang, Qin; Wen, Fuqiang; Zheng, Yuejie; Zhao, Ruizhen; Hu, Qinghua; Zhang, Renli

doi:10.7883/yoken.jjid.2016.328

Cited by 9 publications

(5 citation statements)

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“…The lukAB , whose distribution is unknown due to lack of investigation in a large number of clinical strains, was carried by 67.8% of our isolates. There is a varying carriage of pvl among MRSA, ranging from 2.3 to 50.7% in China [ 42 – 45 ]. In the present study, a relatively low prevalence of pvl -positive isolates (7.9%) was found, which was in agreement with our previous data (6.6%) [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Leukotoxin and pyrogenic toxin Superantigen gene backgrounds in bloodstream and wound Staphylococcus aureus isolates from eastern region of China

He¹,

Xu²,

Zhao³

et al. 2018

BMC Infect Dis

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BackgroundThe bicomponent leukotoxins and the pyrogenic toxin superantigens (PTSAgs) are important virulence factors of Staphylococcus aureus. It is necessary to survey the prevalence and expression of these toxin-encoding genes for understanding the possible pathogenic capacity of S. aureus to cause disease.MethodsFive leukotoxin genes and thirteen PTSAg determinants were detected for 177 S. aureus isolates from blood (n = 88) and wound (n = 89) infections by Polymerase Chain Reaction (PCR). The expression of leukotoxin ED (lukED) was determined by quantitative real-time PCR (qRT-PCR). The genetic backgrounds of isolates were analyzed by Staphylococcal Cassette Chromosome mec (SCCmec) typing (for methicillin-resistant S. aureus isolates), Pulsed-Field Gel Electrophoresis (PFGE), accessory gene regulator (agr) typing and Multilocus Sequence Typing (MLST, for representative isolates based on PFGE type) methods.Results99.4% (176/177) isolates contained at least one of leukotoxin genes. Among them, 94.9% (168/177), 81.4% (144/177) and 67.8% (120/177) isolates harbored hlgBC, lukED and lukAB, respectively. Compared to leukotoxin genes, there was a relatively lower overall prevalence of PTSAg genes [99.4% versus 72.9% (129/177), P < 0.001], and they were organized in 59 patterns, with the most common combination of the egc cluster with or without other PTSAg genes. Genetic analysis showed the distributions of certain toxin genes were associated with the genetic backgrounds of isolates. The egc cluster was a common feature of CC5 isolates, among which ST5 and ST764 isolates harbored more PTSAg genes. The lukED was not present in ST398 isolates, and its expression was quite different among isolates. No significant correlations were observed between the lukED expression levels of strains and the ST or agr types.ConclusionsThe present study elucidated the distribution of leukotoxin and PTSAg genes and the expression of lukED in blood and wound isolates, and analyzed the relationship between them with genetic characteristics of isolates. These data improve the current understanding of the possible pathogenicity of S. aureus.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3297-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Leukotoxin and pyrogenic toxin Superantigen gene backgrounds in bloodstream and wound Staphylococcus aureus isolates from eastern region of China

He¹,

Xu²,

Zhao³

et al. 2018

BMC Infect Dis

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“…According to a previous report, an MRSA CC1-t114-SCCmecIV clone was isolated from SFP outbreaks in China [63]; however, there are few or no reports about this genotype in other countries up to the present. The dominant MRSA lineage was CC59-t437-SCCmecIV/V in cluster III, which was reported as the major clone among CA-MRSA in hospitals and the predominant genotype in bacteremia in China, and also identified in food poisoning outbreaks [46,47]. In addition, the CC59-t437-SCCmecIV/V clone was found in various food types, suggesting a possible diverse dissemination routes of these isolates.…”

Section: Discussionmentioning

confidence: 88%

“…ST59 MRSA were also recovered from humans and pigs in a backyard farm in rural China, indicating a possible bidirectional mode of transmission [45]. ST59 is the most common cause of hospital-acquired CA-MRSA infections, especially in pediatric patients [46,47]. In general, ST59 and ST338 (a single locus variant of ST59) have been shown to be the most common STs associated with community-acquired pneumonia in children in China [48].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance, Virulence, and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Recovered from Ready-to-Eat (RTE) Food in China: A New Challenge for Food Safety

Wang,

Li,

Wang

et al. 2023

Zoonoses

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Objective: The objective of the present study was to determine the prevalence, antimicrobial resistance, virulence profiles, and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) obtained from ready-to-eat (RTE) foods in China. Methods: Two hundred seventy-six RTE food-associated S. aureus isolates were collected from 25 provinces across China in 2018, then characterized by antimicrobial susceptibility testing, virulence factors detecting, multilocus sequence typing (MLST), spa typing, SCCmec typing and pulsed-field gel electrophoresis (PFGE). Results: Two hundred fifty isolates (90.6%) were resistant to at least one antimicrobial agent; 73 (26.4%) isolates were multi-drug resistant (MDR). Thirty MRSA isolates were identified, among which nine toxin genes (sea, seb, sec, sed, seh, selk, sell, selq, and tsst-1) were detected. Sixty percent (18/30) of the MRSA isolates harbored multiple toxin genes. Four virulence gene patterns were identified, with seb-selk-selq (30/30) being the most common pattern. Thirteen sequence types, as well as 13 spa and 4 SCCmec types were found among 30 MRSA isolates. The most prevalent MRSA lineages were CC59-t437-SCCmecIV/V (23.3% [7/30]), CC398-t011-SCCmecV (23.3% [7/30]), and CC1-t114-SCCmecIV (16.7% [5/30]). Conclusions: Our findings highlight the importance for the identification of prevalent clones, assessment of drug-resistance and virulence, and formulation of food safety measures for public health.

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“…In Shenzhen, the main MRSA clone was ST59-SCCmecIV-t437. 27 In Northwest China, the most common clone was ST239-SCCmecIII-t030. 28 Based on published reports, the ST398 typing is a zoonotic clonotype.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Hospitalized Patients in Eastern Heilongjiang Province, China

Yang¹,

Zhao²,

Wang³

et al. 2021

IDR

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Background Recently, owing to antibiotic resistance, the incidence of methicillin-resistant Staphylococcus aureus (MRSA) colonization among intensive care unit (ICU) patients has increased rapidly. So far, there are few studies on active screening of MRSA. The purpose of the current study was to verify the effectiveness of active screening and analyze the molecular epidemiological characteristics of MRSA in the region. Methods We collected 30 samples of the MRSA strains from a tertiary hospital in the Eastern Heilongjiang Province. Among them, 7 were retrieved through nasal vestibular swabs at the emergency ICU and 23 were obtained from clinical specimens. Additionally, relevant patient medical information was examined retrospectively and molecular epidemiology and risk factor analysis for MRSA were performed. Results Molecular epidemiology studies revealed that all strains of bacteria carried the mecA resistance gene. The Panton Valentine leukocidin (PVL), for instance, was detected at a rate of 13.33% (4/30). The Staphylococcus aureus protein A (spa) types, found amongst our samples, were mainly t324, t437, t034, etc., and we discovered a new spa type t19702. We also revealed 3 types of SCCmec, namely, SCCmec type II, SCCmec type IVa, and SCCmec type V, with the most prevalent clonotypes being ST72 and ST59. In addition, we also found 7 new ST types, namely, ST6567, ST6568, ST6569, ST6570, ST6571, ST6572, and ST6573. Using risk factor analysis, we also demonstrated that long, invasive procedures used in the ICU, such as tracheal intubation and ventilator usage, along with patients with cerebral infarction and other embolism are more susceptible to developing MRSA colonization and further infections. Conclusion We recommend the infection control department within hospitals to actively screen for MRSA and perform risk factor analysis in order to establish accurate preventive measures for controlling MRSA spread.

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Antimicrobial Resistance and Molecular Characteristics of Methicillin-Resistant <i>Staphylococcus aureus</i> Isolates from Child Patients of High-Risk Wards in Shenzhen, China

Cited by 9 publications

References 40 publications

Leukotoxin and pyrogenic toxin Superantigen gene backgrounds in bloodstream and wound Staphylococcus aureus isolates from eastern region of China

Leukotoxin and pyrogenic toxin Superantigen gene backgrounds in bloodstream and wound Staphylococcus aureus isolates from eastern region of China

Antimicrobial Resistance, Virulence, and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Recovered from Ready-to-Eat (RTE) Food in China: A New Challenge for Food Safety

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Hospitalized Patients in Eastern Heilongjiang Province, China

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