Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from raw meat in Greater Accra region, Ghana

Dsani, Esther; Afari, Edwin; Danso‐Appiah, Anthony; Kenu, Ernest; Kaburi, Basil Benduri; Egyir, Beverly

doi:10.1186/s12866-020-01935-z

Cited by 35 publications

(32 citation statements)

References 27 publications

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“…Dsani et al [ 111 ] investigated the spread of E. coli isolates from raw meat in Greater Accra region in Ghana, to antibiotics resistance, respectively. Usually, raw meat can be contaminated with antibiotic resistant pathogens and consumption of meat contaminated with antibiotic resistant E. coli is associated with grave health care consequences.…”

Section: Usage Of Antibiotics In Different Countries Of Eu Region mentioning

confidence: 99%

“…Multidrug resistance (MDR) was identified in 22% of the isolates. The bla TEM gene was detected in 4% of E. coli isolates [ 111 ]. Dsani et al [ 111 ] concluded that levels of microbial contamination of raw meat in their research were unacceptable and highlighted that meat handlers and consumers are at risk of foodborne infections from E. coli including ESBL producing E. coli , which is resistant to nearly all antibiotics in use.…”

Section: Usage Of Antibiotics In Different Countries Of Eu Region mentioning

confidence: 99%

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Antimicrobial Resistance in Escherichia coli Strains Isolated from Humans and Pet Animals

Puvača

Frutos

2021

Antibiotics

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Throughout scientific literature, we can find evidence that antimicrobial resistance has become a big problem in the recent years on a global scale. Public healthcare systems all over the world are faced with a great challenge in this respect. Obviously, there are many bacteria that can cause infections in humans and animals alike, but somehow it seems that the greatest threat nowadays comes from the Enterobacteriaceae members, especially Escherichia coli. Namely, we are witnesses to the fact that the systems that these bacteria developed to fight off antibiotics are the strongest and most diverse in Enterobacteriaceae. Our great advantage is in understanding the systems that bacteria developed to fight off antibiotics, so these can help us understand the connection between these microorganisms and the occurrence of antibiotic-resistance both in humans and their pets. Furthermore, unfavorable conditions related to the ease of E. coli transmission via the fecal–oral route among humans, environmental sources, and animals only add to the problem. For all the above stated reasons, it is evident that the epidemiology of E. coli strains and resistance mechanisms they have developed over time are extremely significant topics and all scientific findings in this area will be of vital importance in the fight against infections caused by these bacteria.

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Section: Usage Of Antibiotics In Different Countries Of Eu Region mentioning

confidence: 99%

Section: Usage Of Antibiotics In Different Countries Of Eu Region mentioning

confidence: 99%

Antimicrobial Resistance in Escherichia coli Strains Isolated from Humans and Pet Animals

Puvača

Frutos

2021

Antibiotics

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“…These practices pose the risk of bacteria developing resistance to antimicrobials. Furthermore, studies have shown the presence of antimicrobial resistance E. coli in meats and meats products in Ghana [23][24][25]. Dsani et al [23] reported that E. coli isolated from meat sources were resistant to cefuroxime (17%), sulfamethoxazole-trimethoprim (21%), tetracycline (45%) and ampicillin (57%).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, studies have shown the presence of antimicrobial resistance E. coli in meats and meats products in Ghana [23][24][25]. Dsani et al [23] reported that E. coli isolated from meat sources were resistant to cefuroxime (17%), sulfamethoxazole-trimethoprim (21%), tetracycline (45%) and ampicillin (57%). Eibach et al [24] found that E. coli isolates from poultry meat were resistant to ciprofloxacin (21%) and sulfamethoxazole-trimethoprim (32%), and harbored resistance genes such as Bla SHV , bla CTX-M and bla TEM .…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Antimicrobial Susceptibility of Escherichia coli from Raw Meats, Ready-to-Eat Meats, and Their Related Samples in One Health Context

2021

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Meat is an important food source that can provide a significant amount of protein for human development. The occurrence of bacteria that are resistant to antimicrobials in meat poses a public health risk. This study evaluated the occurrence and antimicrobial resistance of E. coli (Escherichia coli) isolated from raw meats, ready-to-eat (RTE) meats and their related samples in Ghana. E. coli was isolated using the USA-FDA Bacteriological Analytical Manual and phenotypic antimicrobial susceptibility test was performed by the disk diffusion method. Of the 200 examined meats and their related samples, 38% were positive for E. coli. Notably, E. coli was highest in raw beef (80%) and lowest in RTE pork (0%). The 45 E. coli isolates were resistant ≥ 50% to amoxicillin, trimethoprim and tetracycline. They were susceptible to azithromycin (87.1%), chloramphenicol (81.3%), imipenem (74.8%), gentamicin (72.0%) and ciprofloxacin (69.5%). A relatively high intermediate resistance of 33.0% was observed for ceftriaxone. E. coli from raw meats, RTE meats, hands of meat sellers and working tools showed some differences and similarities in their phenotypic antimicrobial resistance patterns. Half (51.1%) of the E. coli isolates exhibited multidrug resistance. The E. coli isolates showed twenty-two different resistant patterns, with a multiple antibiotic resistance index of 0.0 to 0.7. The resistant pattern amoxicillin (A, n = 6 isolates) and amoxicillin-trimethoprim (A-TM, n = 6 isolates) were the most common. This study documents that raw meats, RTE meats and their related samples in Ghana are potential sources of antimicrobial-resistant E. coli and pose a risk for the transfer of resistant bacteria to the food chain, environment and humans.

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“…Globally, poultry is considered as the largest sector of the food-producing industry [6]. In addition, poultry is considered to be fed the largest amount of antibiotics and to be an important producer of animal waste, thus significantly affecting the environment and contributing to the emergence of AR and MDR bacteria [7,8].…”

Section: Introductionmentioning

confidence: 99%

Next-Generation Sequencing Based Gut Resistome Profiling of Broiler Chickens Infected with Multidrug-Resistant Escherichia coli

Afridi

Ali

Chang

2020

Animals

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The study was designed to investigate the fecal microbiome and resistome of broiler chickens infected with multidrug-resistant (MDR) Escherichia coli (E. coli). Fecal samples (n = 410) from broiler chickens were collected from thirteen randomly selected sites of Khyber Pakhtunkhwa and screened for the presence of MDR E. coli. Upon initial screening, thirteen (13) MDR E. coli isolates were then subjected to shotgun metagenome next-generation sequencing (NGS). NGS based resistome analysis identified the multidrug efflux pump system-related genes at the highest prevalence (36%) followed by aminoglycoside (26.1%), tetracycline (15.9%), macrolide-lincosamide-streptogramin (9.6%), beta-lactam (6.6%), rifampin (2%), sulphonamide (1.3%), phenicol (0.91%), vancomycin (0.62%), trimethoprim (0.34%), colistin (0.30%), and quinolone (0.33%). The most abundant virulence-associated genes (VAGs) identified were iroN, iutA, iss, and iucA. NGS based taxonomic profiling at the phylum level revealed the predominance of Proteobacteria (38.9%) followed by Firmicutes (36.4%), Bacteroidetes (15.8%), and Tenericutes (8.9%). Furthermore, pathobionts such as E. coli, Salmonella enterica, Klebsiella pneumoniae, and Shigella flexneri belonging to the family Enterobacteriaceae were predominantly found. This study revealed the widespread presence of MDR genes, diverse VAGs, and a dysbiotic gut in the broiler chickens infected with MDR E. coli of Khyber Pakhtunkhwa for the first time using NGS.

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Antimicrobial resistance and molecular detection of extended spectrum β-lactamase producing Escherichia coli isolates from raw meat in Greater Accra region, Ghana

Cited by 35 publications

References 27 publications

Antimicrobial Resistance in Escherichia coli Strains Isolated from Humans and Pet Animals

Antimicrobial Resistance in Escherichia coli Strains Isolated from Humans and Pet Animals

Phenotypic Antimicrobial Susceptibility of Escherichia coli from Raw Meats, Ready-to-Eat Meats, and Their Related Samples in One Health Context

Next-Generation Sequencing Based Gut Resistome Profiling of Broiler Chickens Infected with Multidrug-Resistant Escherichia coli

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