Antimicrobial resistance and virulence genes profiles of
            <i>Arcobacter butzleri</i>
            strains isolated from back yard chickens and retail poultry meat in Chile

Vidal-Veuthey, Boris; Jara, Ronald; Santander, K.; Mella, A.; Ruíz, Susana; Collado, Luís

doi:10.1111/lam.13404

Cited by 8 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result follows those obtained by Vidal‐Veuthey et al. (2021), who reported that all Arcobacter strains were susceptible to four antibiotics evaluated in his study, including erythromycin and ciprofloxacin, tetracycline and gentamicin. Differences in the susceptibility patterns could be explained by the frequency of drugs in animals for treatment and/or prophylaxis, the lack of standardisation for Arcobacter antimicrobial susceptibility tests and the absence of established breakpoints (Rahimi, 2014).…”

Section: Discussionsupporting

confidence: 91%

Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

Lameei

Rahimi

Shakerian

et al. 2022

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Background Arcobacter spp. has been considered an emerging foodborne pathogen and a hazard to human health. The dairy chain has been isolated from different sources; nevertheless, data on Arcobacter occurrence in raw milk and dairy products in Iran are still scant. Objective The present study investigates the prevalence, antimicrobial susceptibility and the presence of virulence genes of Arcobacters species isolated from milk and dairy products. Methods Then, a total of 350 raw milk samples and 400 dairy product samples were collected from dairy supply centers in Isfahan, Iran. Presumptive Arcobacter strains were obtained by enriching samples in Oxoid Arcobacter enrichment broth (AEB) followed by the filtration of enrichment product through 0.45‐μm pore size membrane filters laid onto non‐selective blood at 30°C under microaerophilic conditions. Molecular identification of Arcobacter cryaerophilus and A. butzleri was performed by Polymerase chain reaction (PCR) amplification of the 16S rRNA gene, followed by sequencing. The disc diffusion method was used to determine the antimicrobial susceptibility of isolates. Targeted resistance and virulence genes were detected using multiplex PCR. Results The results show a low recovery rate of Arcobacter spp. in milk. Arcobacters were found in all types of milk, except raw camel milk, but were absent from all dairy products. Arcobacter butzleri was the predominant species in raw milk. Detection of virulence genes shows that all virulence genes targeted were found among A. butzleri, and six (cadF, cj1349, irgA, mviN, pldA, tlyA) were found among A. cryaerophilus. All A. butzleri strains and some A. cryaerophilus strains isolated from milk were resistant to amoxicillin‐clavulanic acid and tetracycline. All A. cryaerophilus isolates from milk were susceptible to gentamycin, streptomycin, erythromycin and ciprofloxacin. The distribution of resistance genes in Arcobacter strains in milk shows that all isolates carried tet(O) and blaOXA‐61 genes. Conclusions In conclusion, the results indicate a low recovery rate of Arcobacter spp. in milk and milk products. However, a significant number of Arcobacter strains with putative virulence genes may be potential pathogens for humans and an overall increase in Arcobacter resistance to first‐line antibiotics. These results highlight the need for regular surveillance of Arcobacter strains in milk and milk products in Iran.

show abstract

Section: Discussionsupporting

confidence: 91%

Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

Lameei

Rahimi

Shakerian

et al. 2022

Veterinary Medicine & Sci

View full text Add to dashboard Cite

show abstract

“…A. butzleri is known to contain numerous virulence genes and to cause intestinal and extra-intestinal infections, that are often self-limited [27,28]. A. butzleri infection can be treated with antibiotics, i.e., β-lactams, fluoroquinolones, macrolides [28].…”

Section: Introductionmentioning

confidence: 99%

“…However, Arcobacter species frequently display a multidrug-resistant profile, hampering the antibiotic treatment of A. butzleri infections. Recent studies have indicated an increase of resistance against fluoroquinolones as well as tetracycline of A. butzleri and A. cryaerophilus isolates from food and aquatic sources [27,29,30].…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments

et al. 2021

View full text Add to dashboard Cite

Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence and the antimicrobial resistance profile of Arcobacter isolates recovered from different aquatic sources. Besides, we searched for Arcobacter spp. in seaweeds and the corresponding seawater samples. Bacteriological and molecular methods applied to 100 samples led to the isolation of 28 Arcobacter isolates from 27 samples. The highest prevalence was detected in rivers followed by artificial ponds, streams, well waters, and spring waters. Seaweeds contained a higher percentage of Arcobacter than the corresponding seawater samples. The isolates were identified as Arcobacter butzleri (96.4%) and Arcobacter cryaerophilus (3.6%). All the isolates showed a multi-drug resistance profile, being resistant to at least three different classes of antibiotics. Molecular analysis of genetic determinants responsible for tetracycline resistance in nine randomly chosen isolates revealed the presence of tetO and/or tetW. This work confirms the occurrence and the continuous emergence of antibiotic-resistant Arcobacter strains in environmental samples; also, the presence of quinolone-resistant Arcobacter spp. in aquatic sources used for water supply and irrigation represents a potential risk for human health.

show abstract

“…Also, 32.6% and 19.7% of the Arcobacter isolates tested against ciprofloxacin and streptomycin, respectively, had inhibition zone diameters below the ECOFF values indicating resistance for both antimicrobials. A recent study on backyard chickens and retail poultry meat in Chile found lower rates of ciprofloxacin, tetracycline, and erythromycin resistance [ 31 ]. The increased resistance rate observed in this study could be due to differences in geographic location and misuse of antibiotics in commercial and smallholder farms in the current study areas [ 8 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-Resistant Arcobacter spp. in commercial and smallholder farm animals in Asante Akim North Municipality, Ghana and Korogwe Town Council, Tanzania: a cross-sectional study

Paintsil,

Ofori,

Akenten

et al. 2023

Gut Pathog

View full text Add to dashboard Cite

Background Arcobacter species are considered emerging foodborne pathogens that can potentially cause serious infections in animals and humans. This cross-sectional study determined the frequency of potentially pathogenic Arcobacter spp. in both commercial and smallholder farm animals in Ghana and Tanzania. A total of 1585 and 1047 (poultry and livestock) samples were collected in Ghana and Tanzania, respectively. Selective enrichment media, along with oxidase and Gram testing, were employed for isolation of suspected Arcobacter spp. and confirmation was done using MALDI-TOF MS. Antibiotic susceptibility was assessed through disk diffusion method and ECOFFs were generated, for interpretation, based on resulting inhibition zone diameters. Results The overall Arcobacter frequency was higher in Ghana (7.0%, n = 111) than in Tanzania (2.0%, n = 21). The frequency of Arcobacter in commercial farms in Ghana was 10.3% (n/N = 83/805), while in Tanzania, it was 2.8% (n/N = 12/430). Arcobacter was detected in only 3.6% (n/N = 28/780) of the samples from smallholder farms in Ghana and 1.5% (n/N = 9/617) of the samples from Tanzania. For commercial farms, in Ghana, the presence of Arcobacter was more abundant in pigs (45.1%, n/N = 37/82), followed by ducks (38.5%, n/N = 10/26) and quails (35.7%, n/N = 10/28). According to MALDI-TOF-based species identification, Arcobacter butzleri (91.6%, n/N = 121/132), Arcobacter lanthieri (6.1%, n/N = 8/132), and Arcobacter cryaerophilus (2.3%, n/N = 3/132) were the only three Arcobacter species detected at both study sites. Almost all of the Arcobacter from Ghana (98.2%, n/N = 109/111) were isolated during the rainy season. The inhibition zone diameters recorded for penicillin, ampicillin, and chloramphenicol allowed no determination of an epidemiological cut-off value. However, the results indicated a general resistance to these three antimicrobials. Multidrug resistance was noted in 57.1% (n/N = 12/21) of the Arcobacter isolates from Tanzania and 45.0% (n/N = 50/111) of those from Ghana. The type of farm (commercial or smallholder) and source of the sample (poultry or livestock) were found to be associated with multi-drug resistance. Conclusions The high levels of MDR Arcobacter detected from farms in both countries call for urgent attention and comprehensive strategies to mitigate the spread of antimicrobial resistance in these pathogens.

show abstract

Antimicrobial resistance and virulence genes profiles of Arcobacter butzleri strains isolated from back yard chickens and retail poultry meat in Chile

Cited by 8 publications

References 31 publications

Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

Occurrence and Antimicrobial Resistance of Arcobacter spp. Recovered from Aquatic Environments

Antibiotic-Resistant Arcobacter spp. in commercial and smallholder farm animals in Asante Akim North Municipality, Ghana and Korogwe Town Council, Tanzania: a cross-sectional study

Contact Info

Product

Resources

About