Antimicrobial resistance and virulence signatures of Listeria and Aeromonas species recovered from treated wastewater effluent and receiving surface water in Durban, South Africa

Olaniran, Ademola O.; Nzimande, Sphephile B T; Mkize, Ndumiso G.

doi:10.1186/s12866-015-0570-x

Cited by 44 publications

(29 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A 100% prevalence of the actA gene, which stimulates actin polymerization and causes L. monocytogenes to spread in the host cells was observed in this study. This rate is quite higher than that observed in the study of [3] which is 95% and much more higher than that obtained in the studies of [55]. The prevalence of the hylA and plcB genes in the present study was 100%.…”

Section: Discussioncontrasting

confidence: 78%

“…In this study, plcA and iap which code for phospholipase and invasion-associated protein respectively, occurred in 100% of the isolates. This is also higher than the prevalence noticed in other studies [53,[55][56][57]. The variations in the prevalence of virulence markers detected in this study and that observed in other studies may be due to the differences in the origin or source of the isolates.…”

Section: Discussioncontrasting

confidence: 69%

See 1 more Smart Citation

Characterization of antibiogram fingerprints in Listeria monocytogenes recovered from irrigation water and agricultural soil samples

Iwu

Okoh

2020

PLoS ONE

View full text Add to dashboard Cite

Listeria monocytogenes (L. monocytogenes) is a foodborne pathogen and the etiologic agent of listeriosis, which can be disseminated within the agricultural environment particularly soil and irrigation water, contaminate farm produce and cause high mortality and morbidity among vulnerable individuals. This study assessed the incidence and antibiogram of L. monocytogenes recovered from irrigation water and agricultural soil samples collected from Chris Hani and Amathole District Municipalities (DMs) in Eastern Cape Province, South Africa. The distribution of presumptive L. monocytogenes in irrigation water and agricultural soil samples was done using the standard plate count method, while polymerase chain reaction (PCR) was used to identify the isolates. The confirmed isolates were screened for 9 key virulence markers using PCR after which they were subjected to antibiotic susceptibility testing against 18 antibiotics used for the alleviation of listeriosis using the disk diffusion method. Relevant putative antibiotic resistance genes in the resistant variants were screened for using PCR. The distribution of L. monocytogenes in irrigation water samples was statistically significant (P � 0.05) and ranged from log 10

show abstract

Section: Discussioncontrasting

confidence: 78%

Section: Discussioncontrasting

confidence: 69%

Characterization of antibiogram fingerprints in Listeria monocytogenes recovered from irrigation water and agricultural soil samples

Iwu

Okoh

2020

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…These genera have often been reported as carriers of antibiotic resistance plasmids in either environmental or clinical settings, such as plasmid mediated carbapenem- and colistin-resistance in Proteus and Providencia isolates. Recent studies have demonstrated the high potential of Aeromonas in carrying multidrug-resistance plasmids [44–46], and in transferring plasmids in various environmental communities [23–25]. Given the ubiquitous presence of Aeromonas in aquatic environments [47,48], Aeromonas might play a significant role in facilitating transfer and maintenance of plasmid-mediated ARGs in environmental communities.…”

Section: Discussionmentioning

confidence: 99%

Plasmids persist in a microbial community by providing fitness benefit to multiple phylotypes

Dechesne

Madsen

et al. 2019

Preprint

View full text Add to dashboard Cite

21The current epidemic of antibiotic resistance has been facilitated by the wide and rapid 22 horizontal dissemination of antibiotic resistance genes (ARGs) in microbial communities. 23Indeed, ARGs are often located on plasmids, which can efficiently shuttle genes across diverse 24 taxa. While the existence conditions of plasmids have been extensively studied in a few model 25 bacterial populations, their fate in complex bacterial communities is poorly understood. Here, 26we coupled plasmid transfer assays with serial growth experiments to investigate the 27 persistence of the broad-host-range IncP-1 plasmid pKJK5 in microbial communities derived 28 from a sewage treatment plant. The cultivation conditions combined different nutrient and 29 oxygen levels, and were non-selective and non-conducive for liquid-phase conjugal transfer. 30Following initial transfer, the plasmid persisted in almost all conditions during a 10-day serial 31 growth experiment (equivalent to 60 generations), with a transient transconjugant incidence up 32 to 30%. By combining cell enumeration and sorting with amplicon sequencing, we mapped 33 plasmid fitness effects across taxa of the microbial community. Unexpected plasmid fitness 34 benefits were observed in multiple phylotypes of Aeromonas, Pseudomonas and 35Enterobacteriaceae, which resulted in community-level plasmid persistence. We demonstrate, 36 for the first time, that plasmid fitness effects across community members can be estimated in a 37 high-throughput way without prior isolation. By gaining a fitness benefit when carrying 38 plasmids, members within complex microbial communities might have a hitherto unrecognized 39 potential to maintain plasmids for long-term community-wide access. 40 41 Introduction 42Plasmids -extrachromosomal replicons -can support rapid bacterial adaptation by moving 43 genes between phylogenetically diverse bacteria, a process known as horizontal gene transfer 44[1]. This is particularly important in the case of antibiotic resistance, where acquisition of 45 plasmid-borne antibiotic resistance genes (ARGs) can instantly render a strain impervious to 46 antibiotic treatment [2]. The current global antibiotic resistance crisis has been largely 47 attributed to plasmid mediated ARG dissemination [3]. 48 49Strategies to combat the antibiotic resistance epidemic therefore require an understanding of 50 the mechanisms that underlie plasmid fate in microbial communities. Upon entering a 51 microbial community, a plasmid will only persist if its original or secondary hosts 52 (transconjugants) survive. If it persists, it will constitute a long-term reservoir of auxiliary 53 genes for that community. In fact, persistence of IncP-1 plasmids or their derivatives has 54 recently been observed in ex situ communities like anaerobic sludge microcosms [4] and 55 artificial multi-species system [5] over periods longer than 10 days, as well as in murine gut 56 microbiota experiments [6]. However, by simply interpreting plasmid persistence as the 57 frequency of plasmid ...

show abstract

“…Some studies suggest that Listeria species readily survive conventional wastewater treatment processes even after tertiary treatment [10,15]. With reports of inadequate removal of Listeria pathogens from wastewater from the developed world [10], it can be presumed that wastewater treatment plants in developing countries may be inefficient for the removal of these pathogens from wastewater effluents prior to discharge into the receiving water bodies.…”

Section: Introductionmentioning

confidence: 99%

Listeria Species Isolated from the Wastewater Samples of a Private and Tertiary Hospital in Ibadan and their Antibiotics Resistance Patterns

Falodun

Amusan

2018

JAMB

View full text Add to dashboard Cite

Introduction: In hospitals, a significant amount of water is consumed and equally, significant amounts of wastewater is disposed with high levels of contaminants, including disease-causing bacteria such as Listeria spp. have been found in wastewater effluent and surrounding freshwater bodies. Recent studies suggest that Listeria species readily survive conventional wastewater treatment processes even after tertiary treatment. This study was carried out to determine the antibiotic resistance pattern of Listeria spp. isolated from hospital wastewater (treated and untreated) from private and tertiary hospital samples in Ibadan and comment on the public health significance. Materials and Methods: Hospital wastewater samples were collected between April and July, 2016. Listeria Selective Agar Base with Listeria Selective Supplement (Oxoid, UK) was used for the isolation of the Listeria species and the isolates were identified using standard conventional methods. Antimicrobial susceptibility testing was done against ampicillin (10 µg), cloxacillin (5 µg), amoxicillin (5 µg), streptomycin (10 µg), ceftriaxone (30 µg), chloramphenicol (30 µg), ciprofloxacin (5 µg), ofloxacin (5 µg), sulfamethoxazole-trimethoprim (25 µg) and tetracycline (30 µg) by the

show abstract

Antimicrobial resistance and virulence signatures of Listeria and Aeromonas species recovered from treated wastewater effluent and receiving surface water in Durban, South Africa

Cited by 44 publications

References 61 publications

Characterization of antibiogram fingerprints in Listeria monocytogenes recovered from irrigation water and agricultural soil samples

Characterization of antibiogram fingerprints in Listeria monocytogenes recovered from irrigation water and agricultural soil samples

Plasmids persist in a microbial community by providing fitness benefit to multiple phylotypes

Listeria Species Isolated from the Wastewater Samples of a Private and Tertiary Hospital in Ibadan and their Antibiotics Resistance Patterns

Contact Info

Product

Resources

About