Antimicrobial Resistance in Isolates from Cattle with Bovine Respiratory Disease in Bavaria, Germany

Melchner, Alexander; Berg, Sarah van de; Scuda, Nelly; Feuerstein, Andrea; Hanczaruk, Matthias; Schumacher, Magdalena; Straubinger, Reinhard K.; Marosevic, D; Riehm, Julia M.

doi:10.3390/antibiotics10121538

Cited by 7 publications

(3 citation statements)

References 48 publications

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“…Economic losses due to treatment costs and reduced weight gain in beef production [55,56], as well as decreased milk yield and other negative effects on dairy cow health [57][58][59], highlight the importance of treatment options for infections caused by these two pathogens; especially, since multidrug-resistant (MDR) isolates are increasingly isolated from cattle with bovine respiratory disease (BRD). In Germany, the rates of MDR isolates have increased more than six-fold in P. multocida within a five-year period (2015-2020) [60]. That study reported MDR rates of 13.9% and 5.1%, while rates of pan-susceptibility were only 15.1% and 12.1% in isolates of P. multocida and M. haemolytica, respectively [60].…”

Section: Discussionmentioning

confidence: 98%

“…In Germany, the rates of MDR isolates have increased more than six-fold in P. multocida within a five-year period (2015-2020) [60]. That study reported MDR rates of 13.9% and 5.1%, while rates of pan-susceptibility were only 15.1% and 12.1% in isolates of P. multocida and M. haemolytica, respectively [60]. Another recent study identified a novel high-level macrolide resistance determinant in M. haemolytica, further limiting treatment options for BRD [61].…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Antibacterial Activity of Microbial Natural Products against Bacterial Pathogens of Veterinary and Zoonotic Relevance

Barth,

Preussger,

Pietschmann

et al. 2024

Antibiotics

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Antimicrobial resistance (AMR) is considered one of the greatest threats to both human and animal health. Efforts to address AMR include implementing antimicrobial stewardship programs and introducing alternative treatment options. Nevertheless, effective treatment of infectious diseases caused by bacteria will still require the identification and development of new antimicrobial agents. Eight different natural products were tested for antimicrobial activity against seven pathogenic bacterial species (Brachyspira sp., Chlamydia sp., Clostridioides sp., Mannheimia sp., Mycobacterium sp., Mycoplasma sp., Pasteurella sp.). In a first pre-screening, most compounds (five out of eight) inhibited bacterial growth only at high concentrations, but three natural products (celastramycin A [CA], closthioamide [CT], maduranic acid [MA]) displayed activity at concentrations <2 µg/mL against Pasteurella sp. and two of them (CA and CT) also against Mannheimia sp. Those results were confirmed by testing a larger collection of isolates encompassing 64 Pasteurella and 56 Mannheimia field isolates originating from pigs or cattle, which yielded MIC90 values of 0.5, 0.5, and 2 µg/mL against Pasteurella and 0.5, 4, and >16 µg/mL against Mannheimia for CA, CT, and MA, respectively. CA, CT, and MA exhibited higher MIC50 and MIC90 values against Pasteurella isolates with a known AMR phenotype against commonly used therapeutic antimicrobial agents than against isolates with unknown AMR profiles. This study demonstrates the importance of whole-cell antibacterial screening of natural products to identify promising scaffolds with broad- or narrow-spectrum antimicrobial activity against important Gram-negative veterinary pathogens with zoonotic potential.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

In Vitro Antibacterial Activity of Microbial Natural Products against Bacterial Pathogens of Veterinary and Zoonotic Relevance

Barth,

Preussger,

Pietschmann

et al. 2024

Antibiotics

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“…Bovine respiratory disease (BRD), the leading cause of morbidity and mortality in U.S. beef cattle ( 1 ), poses a threat to cattle operations. The prevalence of antimicrobial resistance (AMR) appears to be increasing in Mannheimia haemolytica ( M. haemolytica ), a leading contributor to BRD ( 2 – 4 ); research is underway to determine the causes and impact of AMR in M. haemolytica . Characterization of the AMR phenotype by culture and antimicrobial susceptibility testing is complicated by uncertainty regarding the number of M. haemolytica colonies that must be selected to adequately characterize antimicrobial susceptibility phenotypes (antibiograms) and genotypes in a sample.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Diversity of Antimicrobial Resistance Phenotypes and Genotypes of Mannheimia haemolytica Isolates From Bovine Nasopharyngeal Swabs

et al. 2022

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The threat of bovine respiratory disease (BRD) for cattle operations is exacerbated by increasing prevalence of antimicrobial resistance (AMR) in Mannheimia haemolytica, a leading cause of BRD. Characterization of AMR in M. haemolytica by culture and susceptibility testing is complicated by uncertainty regarding the number of colonies that must be selected to accurately characterize AMR phenotypes (antibiograms) and genotypes in a culture. The study objective was to assess phenotypic and genotypic diversity of M. haemolytica isolates on nasopharyngeal swabs (NPS) from 28 cattle at risk for BRD or with BRD. NPS were swabbed onto five consecutive blood agar plates; after incubation up to 20 M. haemolytica colonies were selected per plate (up to 100 colonies per NPS). Phenotype was determined by measuring minimum inhibitory concentrations (MIC) for 11 antimicrobials and classifying isolates as resistant or not. Genotype was indirectly determined by matrix-assisted laser desorption/ionization time of flight mass spectroscopy (MALDI-TOF MS). NPS from 11 of 28 cattle yielded at least one M. haemolytica isolate; median (range) of isolates per NPS was 48 (1–94). NPS from seven cattle yielded one phenotype, 3 NPS yielded two, and 1 NPS yielded three; however, within a sample all phenotypic differences were due to only one MIC dilution. On each NPS all M. haemolytica isolated were the same genotype; genotype 1 was isolated from three NPS and genotype two was isolated from eight. Diversity of M. haemolytica on bovine NPS was limited, suggesting that selection of few colonies might adequately identify relevant phenotypes and genotypes.

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Pasteurella multocida from deep nasal swabs and tracheobronchial lavage in bovine calves from Sweden

Myrenås,

Pringle,

Harbom

et al. 2024

Acta Vet Scand

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Background Bovine respiratory disease (BRD) is common in intensively raised cattle and is often treated with antibiotics. For practitioners, knowledge of the bacteria involved in an outbreak and their antibiotic susceptibility is warranted. To this end, samples from the upper or lower respiratory tract of calves can be submitted for bacteriological culture and susceptibility testing of relevant isolates. However, it is debated whether isolates from the upper respiratory tract are representative of bacteria causing infections in the lower respiratory tract. In this study, we used MALDI-TOF MS, multilocus sequence typing (MLST) and core-genome multilocus sequence typing (cgMLST) to compare culture results of 219 paired samples (sample pairs) of deep nasal swabs (DNS) and tracheobronchial lavage (TBL). The sample pairs came from 171 calves in 30 calf groups across 25 farms with 48 calves sampled twice. Results The predominant bacterial pathogen was Pasteurella multocida, which was isolated from 37.4% of DNS and 22.4% of TBL. There was no statistically significant difference in isolation frequency of P. multocida between calves considered healthy and those suspected for BRD for DNS (P = 0.778) or TBL (P = 0.410). Among the 49 sample pairs where P. multocida was isolated from TBL, the same species was isolated from DNS in 29 sample pairs (59.2%). Isolates from 28 of these sample pairs were evaluated by MLST, and in 24 pairs (86.0%) P. multocida from DNS and TBL were of the same sequence type (ST). Moreover, cgMLST showed that the genetic distance between isolates within 21 of the 28 sample pairs (75.0%), was less than two alleles, and DNS and TBL isolates were considered identical. In seven sample pairs (25%), the genetic distance was greater, and DNS and TBL isolates were considered nonidentical. Conclusions Pasteurella multocida was readily isolated from DNS and in calves where this species was isolated also from TBL, DNS and TBL isolates were identical in 75% of the sample pairs. This suggests that during an outbreak of BRD, submission of DNS samples from 4 to 6 calves could be a convenient approach for practitioners seeking guidance on P. multocida present in the lower respiratory tract and their antibiotic susceptibility.

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Antimicrobial Resistance in Isolates from Cattle with Bovine Respiratory Disease in Bavaria, Germany

Cited by 7 publications

References 48 publications

In Vitro Antibacterial Activity of Microbial Natural Products against Bacterial Pathogens of Veterinary and Zoonotic Relevance

In Vitro Antibacterial Activity of Microbial Natural Products against Bacterial Pathogens of Veterinary and Zoonotic Relevance

Assessment of Diversity of Antimicrobial Resistance Phenotypes and Genotypes of Mannheimia haemolytica Isolates From Bovine Nasopharyngeal Swabs

Pasteurella multocida from deep nasal swabs and tracheobronchial lavage in bovine calves from Sweden

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