Antimicrobial resistance, integrons and plasmid replicon typing in multiresistant clinical <i>Escherichia coli</i> strains from Enugu State, Nigeria

Chah, Kennedy F.; Agbo, Ifeoma C.; Eze, Didacus Chukwuemeka; Somalo, Sergio; Estepa, Vanesa; Torres, Carmén

doi:10.1002/jobm.200900325

Cited by 25 publications

(24 citation statements)

References 38 publications

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“…However, there is a paucity of data on prevalence and antimicrobial resistant E. coli from food-producing animals that may also have a role in the dissemination of antimicrobial resistance among humans in Nigeria. 4,6,15,21,26 This information is essential to determine the possibility of spread of antimicrobial resistance to the human population from healthy foodproducing animals at slaughter or for sale to the public. In this study, the prevalence of E. coli from healthy cattle, chickens, and swine and antimicrobial resistance profiles, including MDR patterns among the isolates, was determined.…”

Section: Discussionmentioning

confidence: 99%

“…11,13 Although there are a number of studies on antimicrobial resistance in E. coli from food animals in the western world, there is a paucity of data on prevalence of multi-drug resistant (MDR; resistance to ‡ 2 antimicrobials) E. coli from food animals in developing countries such as Nigeria. 4,6,21,24 This information is useful to compare trends in resistance in countries where antimicrobial use in both humans and animals is poorly regulated. In Nigeria, the major sources of meat consumed are beef and poultry and food-producing animals serve as major sources of meat to the population of Lagos State, Nigeria, which currently comprises more than 21 million people (www.lagosstate.nig).…”

Section: Introductionmentioning

confidence: 99%

“…11 Furthermore, MDR E. coli from food animals are prevalent in Nigeria as reported in one study where 71 different MDR types were identified in E. coli from cattle. 4 Although prevalence of commensal E. coli and their antimicrobial resistance patterns were determined in previous studies, 4,6,21,24 few of those studies included E. coli from all major meat animals consumed in Nigeria and none tested the isolates against a wide range of antimicrobials primarily used for treating human infections. The purpose of this study was to determine prevalence and antimicrobial resistance of E. coli isolated from chicken, cattle, and swine in Lagos, Nigeria.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies on prevalence and antimicrobial resistance of commensal E. coli from food animals from different parts of Nigeria have reported high levels of resistance. 4,6,21,24 Of particular concern is resistance to antimicrobials such as aminoglycosides, cephalosporins, fluoroquinolones, and sulfonamides that are commonly used to treat human infections caused by Gram-negative bacteria. 11 Furthermore, MDR E. coli from food animals are prevalent in Nigeria as reported in one study where 71 different MDR types were identified in E. coli from cattle.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and Antimicrobial Resistance inEscherichia colifrom Food Animals in Lagos, Nigeria

Adenipekun

Jackson

Oluwadun

et al. 2015

Microbial Drug Resistance

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Foodborne bacteria are often associated with human infections; these infections can become more complicated to treat if the bacteria are also resistant to antimicrobials. In this study, prevalence, antimicrobial resistance, and genetic relatedness of Escherichia coli among food producing animals from Lagos, Nigeria, was investigated. From December 2012 to June 2013, E. coli were isolated from fecal samples of healthy cattle, chicken, and swine. Antimicrobial susceptibility testing against 22 antimicrobials was performed using broth microdilution with the Sensititre™ system. Clonal types were determined by pulsed-field gel electrophoresis (PFGE). From the analysis, 211/238 (88.7%), 170/210 (81%), and 136/152 (89.5%) samples from cattle, chicken, and swine, respectively, were positive for E. coli. A subset of those isolates (n=211) selected based on β-lactamase production was chosen for further study. Overall, E. coli exhibited the highest resistance to tetracycline (124/211; 58.8%), trimethoprim/sulfamethoxazole (84/211; 39.8%), and ampicillin (72/211; 34.1%). Approximately 40% of the isolates were pan-susceptible, and none of the isolates were resistant to amikacin, cefepime, ceftazidime, ertapenem, meropenem, or tigecycline. Among the resistant isolates, 28 different resistance patterns were observed; 26 of those were characterized as multi-drug resistant (MDR; resistance to ≥2 antimicrobials). One isolate was resistant to 13 different antimicrobials representing five different antimicrobial classes. Using PFGE, MDR E. coli were genetically diverse and overall did not group based on source; identical PFGE patterns were detected among isolates from different sources. These results suggest that isolates cannot be attributed to specific sources, and some may be present across all of the sources. Results from this study indicate that food-producing animals in Nigeria are a reservoir of MDR E. coli that may be transferred to humans via the food chain.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence and Antimicrobial Resistance inEscherichia colifrom Food Animals in Lagos, Nigeria

Adenipekun

Jackson

Oluwadun

et al. 2015

Microbial Drug Resistance

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“…This finding could suggest that resistance among E. coli cultured from broiler chickens in Australia, regardless of their pathogenicity, was not due to clonal spread but most likely due to the transmission of ARGs (Chah et al, 2010). However, not all of the isolates were pathogenic, 187 were cultured from the faeces of healthy birds.…”

Section: Discussionmentioning

confidence: 97%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Benzalkonium Chloride

Kampf

2024

Antiseptic Stewardship

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Antimicrobial resistance, integrons and plasmid replicon typing in multiresistant clinical Escherichia coli strains from Enugu State, Nigeria

Cited by 25 publications

References 38 publications

Prevalence and Antimicrobial Resistance inEscherichia colifrom Food Animals in Lagos, Nigeria

Prevalence and Antimicrobial Resistance inEscherichia colifrom Food Animals in Lagos, Nigeria

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Benzalkonium Chloride

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