Antimicrobial resistance of Escherichia coli O157 from cattle in Korea

You, Ju-Yeon; Moon, Bo-Mi; Oh, In-Gyeong; Baek, Byeong Kirl; Li, Liguang; Kim, Byeong Su; Stein, Barry D.; Lee, John Hwa

doi:10.1016/j.ijfoodmicro.2005.05.013

Cited by 16 publications

(21 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to the data from the present study, they did not observe resistance of enrofloxacin in studied coli strains. In Korea, You et al (44) determined that cattle EHEC 0157 were most frequently resistant to streptomycin, tetracycline, sulfamethoxazole, ampicillin, kanamycin, cefalotin, ticarcillin and sulfamethoxazole.…”

Section: Discussionmentioning

confidence: 99%

Investigations on Prevalence and Antimicrobial Resistance of Enterohaemorrhagic Escherichia Coli (Ehec) Among Dairy Farms in the North Part of the Republic of Bulgaria

Urumova

Lyutzkanov

Petrov

2015

Macedonian Veterinary Review

View full text Add to dashboard Cite

, a total of 1094 faecal swab samples were collected from cattle at different age at 4 farms in North Bulgaria: Okorsh, Slavyanovo (Popovo municipality), Dobri dol and Trem. Out of them, 36 coli strains (3.3%) positive in the E. coli O:157 antiserum agglutination test and identified by the BBL CRYSTAL identification system as belonging to the E. coli O:157 serotype were isolated. The distribution of isolates was as followed: 5 (0.5%) E. coli O:157 strains at the Okorsh dairy cattle farm, 7 (0.6%) E. coli isolates at the Slavyanovo dairy farm, 16 (1.5%) isolates at the Dobri dol farm and 8 (0.7%) isolates at the Trem farm. Colibacteria exhibited 100% sensitivity to oxyimino-cephalosporins, gentamicin and enrofloxacin, and were resistant to ampicillin (19.4%) and tetracycline (41.6%). From the 15 strains resistant to tetracycline, 11 were isolated from the cows at Dobri dol, while the other 4 originated from the other three farms. The 7 ampicillin-resistant E. coli isolates were detected only at the Dobri dol cattle farm.

show abstract

Section: Discussionmentioning

confidence: 99%

Investigations on Prevalence and Antimicrobial Resistance of Enterohaemorrhagic Escherichia Coli (Ehec) Among Dairy Farms in the North Part of the Republic of Bulgaria

Urumova

Lyutzkanov

Petrov

2015

Macedonian Veterinary Review

View full text Add to dashboard Cite

show abstract

“…Later, non-sorbital fermenting (NSF) colonies were selected from MacConkey agar with sorbitol, cefixime, and tellurite (CT-SMAC) plates and streaked onto eosin methylene blue (EMB) agar plates, which were further incubated at 37 C for 24 hr. On EMB, typical E. coli with a metallic shine were further characterized by biochemical tests (indole, methyl red, vogesproskauer, citrate, and lysine decarboxylase tests) as reported previously (Lee & Choi, 2006;You et al, 2006). For further confirmation that the isolated colonies were E. coli, the Remel RapID ONE System (Thermo Fisher Scientific, U.K.) was also used, which identifies medically important Enterobacteriaceae and other oxidase-negative Gram-negative bacilli based on enzyme technology.…”

Section: Culture Conditions and Biochemical Identificationmentioning

confidence: 99%

Multiplex polymerase chain reaction detection of Shiga toxin genes and antibiotic sensitivity of Escherichia coli O157:H7 isolated from beef meat in Quetta, Pakistan

Samad

Abbas

Ahmad

et al. 2018

Journal of Food Safety

View full text Add to dashboard Cite

The objective of this study was to conduct a qualitative analysis of raw beef meat sold in the city of Quetta, Pakistan for presence and drug sensitivity of the potentially pathogenic Escherichia coli strain O157:H7. The study used 200 raw beef meat samples collected from retail butcher shops. Conventional and rapid biochemical tests, latex agglutination and multiplex polymerase chain reaction (PCR) using primers designed for the rfb O157 and flic H7 genes were used to detect E. coli O157:H7. All O157:H7 isolates were also tested for Shiga toxin genes stx 1 and stx 2. The prevalence of E. coli O157:H7 in collected beef meat samples was 10%. Detection through PCR was found more sensitive than detection of O and H antigens. The quantity of E. coli O157:H7 isolates positive for Shiga toxins was 50% (20% for stx 1, 45% for stx 2 and 10% for both stx 1 and stx 2). Season wise variation showed highest E. coli O157:H7 prevalence during summer months. A further concern is that E. coli O157:H7 isolates were resistant to a range of common antibiotics. The results indicate an urgent need for applying proper food hygiene practices in the Quetta region to reduce incidence of foodborne diseases and they also emphasize the global problem of antimicrobial resistance. Practical applications E. coli O157:H7 is as a potentially threatening foodborne pathogen. A significant prevalence of E. coli O157:H7 detected in raw beef meat from retail outlets in the city of Quetta indicates an urgent need for applying proper food hygiene practices in the Quetta region to reduce the incidence of foodborne diseases. Furthermore, resistance of the E. coli O157:H7 isolates to a range of commonly used antibiotics emphasizes the global problem of antimicrobial resistance. The multiplex PCR method used here is a reliable, sensitive, and relatively rapid technique for detecting E. coli O157:H7 in food and environmental samples and important for ongoing surveillance to minimize contamination of raw meat products and associated cross contamination by E. coli O157:H7.

show abstract

“…Strain LFH34, the bovine E. coli O157:H7 isolate that was PCR-negative for all three virulence genes, was the only isolate that was sensitive to all antimicrobials tested. STEC isolates displaying resistance to multiple antimicrobials have been also previously reported from different parts of the world (Giammanco et al 2002;You et al 2006;Walsh et al 2006;Solomakos et al 2009). Although the antibiotic treatment of STEC-infected patients is considered controversial (Wong et al 2000;Serna and Boedeker 2008), the monitoring of antimicrobial resistance in STEC isolates may still be useful for both epidemiological purposes and for the monitoring of the spread of antimicrobial resistance among different microbial species.…”

Section: Isolation Of E Coli O157 and Detection Of Virulence Genesmentioning

confidence: 80%

“…STEC isolates displaying resistance to multiple antimicrobials have been also previously reported from different parts of the world (Giammanco et al . 2002; You et al . 2006; Walsh et al .…”

Section: Resultsmentioning

confidence: 99%

OCCURRENCE, VIRULENCE GENES AND ANTIMICROBIAL RESISTANCE OF ESCHERICHIA COLI O157 IN BOVINE, CAPRINE, OVINE AND PORCINE CARCASSES IN GREECE

Govaris

Angelidis

Κatsoulis

et al. 2011

Journal of Food Safety

View full text Add to dashboard Cite

One thousand and two hundred carcasses (620 bovine, 130 caprine, 230 ovine and 220 porcine) from several slaughterhouses throughout Greece were examined for the presence of Escherichia coli O157 after evisceration and before chilling. Twelve E. coli O157 strains (1.0%) were isolated of which eight were from bovine (1.3%), one from caprine (0.8%) and three from ovine (1.3%) meat. None was isolated from pork meat. Six out of the 12 E. coli O157 isolates (50.0%) could be classified as Shiga‐toxigenic based on polymerase chain reaction (PCR), belonged to the E. coli O157:H7 serotype and were PCR‐positive for the stx1, stx2 and eae genes except one isolate that was stx1‐negative. The remaining six E. coli O157 isolates tested negative for Shiga‐toxin production, both by immunoassay and by PCR, were PCR‐negative for the eae gene, and all except one (strain LFH34, a bovine carcass isolate) were also PCR‐negative for the H7 flagellar gene. Among the 15 antimicrobials tested, the E. coli O157 isolates were the most frequently resistant to ticarcillin (9 out of the 12 isolates, 75%). PRACTICAL APPLICATIONS The Shiga toxin‐producing Escherichia coli (STEC) strains are severe human pathogens. Although the prevalence of STEC in bovine meat has been investigated in several countries worldwide, no such study has been conducted previously in Greece. In addition, very few studies in the literature have reported on the prevalence and characterization of STEC isolated from animal carcasses other than bovine. The findings of the present study are also useful for epidemiological purposes (monitoring the evolution and spread of STEC worldwide).

show abstract

Antimicrobial resistance of Escherichia coli O157 from cattle in Korea

Cited by 16 publications

References 22 publications

Investigations on Prevalence and Antimicrobial Resistance of Enterohaemorrhagic Escherichia Coli (Ehec) Among Dairy Farms in the North Part of the Republic of Bulgaria

Investigations on Prevalence and Antimicrobial Resistance of Enterohaemorrhagic Escherichia Coli (Ehec) Among Dairy Farms in the North Part of the Republic of Bulgaria

Multiplex polymerase chain reaction detection of Shiga toxin genes and antibiotic sensitivity of Escherichia coli O157:H7 isolated from beef meat in Quetta, Pakistan

OCCURRENCE, VIRULENCE GENES AND ANTIMICROBIAL RESISTANCE OF ESCHERICHIA COLI O157 IN BOVINE, CAPRINE, OVINE AND PORCINE CARCASSES IN GREECE

Contact Info

Product

Resources

About